Pharmacological modulation of allergic inflammation in the rat airways and association with mast cell heterogeneity


Autoria(s): Damazo, A. S.; de Lima, W. T.; Perretti, M.; Oliani, S. M.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

24/08/2001

Resumo

Administration of ovalbumin by aerosol to sensitised rats produced a rapid (15 min) protein exudation in different airway tissues, as determined by Evans blue staining. This was associated with marked mast cell degranulation determined by histological examination, with there being no difference between mucosal and connective tissue mast cells. A 5-day administration regimen with compound 48/80 selectively depleted connective tissue mast cell (Positive to berberine staining) without modifying ovalbumin-induced plasma protein extravasation. Treatment of rats with dexamethasone (1 mg/kg, - 12 h) or nor-dihydroguaiaretic acid (30 mg/kg i.p., - 30 min) significantly reduced ovalbumin-induced protein extravasation and preserved mucosal mast cell morphology. Indomethacin (4 mg/kg i.v., - 30 min) exerted no effect on either parameter. In conclusion, we propose the mucosal mast cell as a target cell responsible at least partly for the inhibitory actions of known anti-inflammatory drugs. We suggest an involvement of endogenous leukotriene(s), but not prostanoid(s), in mucosal mast cell activation/degranulation. (C) 2001 Published by Elsevier B.V. B.V.

Formato

123-130

Identificador

http://dx.doi.org/10.1016/S0014-2999(01)01093-7

European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 426, n. 1-2, p. 123-130, 2001.

0014-2999

http://hdl.handle.net/11449/32539

10.1016/S0014-2999(01)01093-7

WOS:000170879000017

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

European Journal of Pharmacology

Direitos

closedAccess

Palavras-Chave #dexamethasone #indomethacin #nor-dihydroguaiaretic acid #plasma protein extravasation #compound 48/80 #mast cell
Tipo

info:eu-repo/semantics/article