The defensive functions of plant inhibitors are not restricted to insect enzyme inhibition
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2010
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Resumo |
Three plant proteinase inhibitors BbKI (kallikrein inhibitor) and BbCI (cruzipain inhibitor) from Bauhinia bouhinioides, and a BrTI (trypsin inhibitor) from B. rufa, were examined for other effects in Callosobruchus maculatus development; of these only BrTI affected bruchid emergence. BrTI and BbKI share 81% identities in their primary sequences and the major differences between them are the regions comprising the RGD and RGE motifs in BrTI. These sequences were shown to be essential for BrTI insecticidal activity, since a modified BbKI [that is a recombinant form (BbKIm) with some amino acid residues replaced by those found in BrTI sequence] also strongly inhibited insect development. By using synthetic peptides related to the BrTI sequence, YLEAPVARGDGGLA-NH(2) (RGE) and IVYYPDRGETGL-NH(2) (RGE), it was found that the peptide with an RGE sequence was able to block normal development of C. maculatus larvae (ED(50) 0.16% and LD(50) 0.09%), this being even more effective than the native protein. (C) 2009 Elsevier Ltd. All rights reserved. CAPES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CNPq Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP FAP/FADA (UNIFESP) FAP/FADA (UNIFESP) |
Identificador |
PHYTOCHEMISTRY, v.71, n.2/Mar, p.214-220, 2010 0031-9422 http://producao.usp.br/handle/BDPI/30149 10.1016/j.phytochem.2009.10.009 |
Idioma(s) |
eng |
Publicador |
PERGAMON-ELSEVIER SCIENCE LTD |
Relação |
Phytochemistry |
Direitos |
restrictedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
Palavras-Chave | #Bauhinia sp. #Fabaceae #Caesalpinodae #Callosobruchus maculatus #Vigna unguiculata #Leguminosae #Cowpea #Defense protein #Insect attack #Kunitz inhibitors #Pesticide #Plant peptidase inhibitors #RGD #Trypsin inhibitor #CALLOSOBRUCHUS-MACULATUS COLEOPTERA #PROTEINASE-INHIBITORS #BACILLUS-THURINGIENSIS #PLASMA KALLIKREIN #MIDGUT #RESISTANCE #BRUCHIDAE #TOXIN #SEEDS #Biochemistry & Molecular Biology #Plant Sciences |
Tipo |
article original article publishedVersion |