30 resultados para BRUCHIDAE


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Three bruchid pest species, Callosobruchus maculatus, Callosobruchus chinensis and Callosobruchus rhodesianus, were studied for their response to insecticide toxicity taking into account the separate and interactive effects of temperature and pre-adult food. The food types used were cowpea (Vigna unguiculata) and mungbean (Vigna radiata). Callosobruchus maculatus was the most tolerant to malathion and the least affected by temperature change while C. rhodesianus was the least tolerant. Over a 4 C range (23, 25, 27 C), there was generally a significant impact of temperature on the tolerance of the three species to the insecticide. The food type on which the insects developed influenced considerably the degree of insecticide tolerance. Callosobruchus maculatus and C. chinensis populations reared onmungbean had higher tolerance to malathion than their counterparts reared on cowpea, but the opposite was observed in C. rhodesianus populations. The food influence in this study suggested an ancestral cause or fitness cost depending on the species. The interaction of food-by-temperature had no significant effect on malathion toxicity to this genus. Correlation analysis showed C. chinensis to be relatively less sensitive to insecticide concentration over the range studied compared with the other two species.

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The impact of cowpea variety on the response of cowpea bruchid, Callosobruchus maculatus, to malathion was investigated. The interaction of six cowpea varieties (Adamawa Brown, Ife BPC, Ife Brown, Lilongwe, Ntcheu and NCRI-L25) with the geographical strains of C. maculatus (Brazil and Cameroon), temperature (23, 25, 27 C) and insecticide concentration were considered. Cowpea variety (V) had an unpredictable effect on C. maculatus response to malathion. Bruchid populations produced by Ife BPC were the most susceptible to malathion while those yielded by NCRI-L25 were the most tolerant. Regardless of the cowpea variety, the Brazil strain showed higher tolerance than the Cameroon strain. There was significant effect of temperature (T) and insecticide concentration (C) on malathion tolerance in both strains (S). Likewise, there was significant impact of all two-way interactions on cowpea bruchid tolerance except V x C. Significant three-way interactions on C. maculatus tolerance to malathion was only observed in S T V and S T C. The predictability of changing one of the factors on the susceptibility of C. maculatus to insecticide was very low. This study suggests a need to take the insecticide tolerance of insect populations produced by novel varieties into account during plant breeding in addition to factors such as yield and resistance to insect and disease attack.

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BACKGROUND: Bruchid beetles, Callosobruchus species, are serious pests of economically important grain legumes; their activity in stores is often controlled by use of synthetic insecticides. Esterases are known to be involved in insecticide resistance in insects. However, there is dearth of information on esterase activity in the genus Callosobruchus. In this study we investigated the effect of species, geographical strain and food type on the variation of acetylcholinesterase (AChE) activity and its inhibition by malaoxon (malathion metabolite) using an in vitro spectrophotometric method. RESULT: AChE activity varied significantly among species and strains and also among legume type used for rearing them. Generally irrespective of species, strain or food type, the higher the AChE activity of a population, the higher its inhibition by malaoxon. C. chinensis had the highest AChE activity of the species studied and in the presence of malaoxon it had the lowest remaining AChE activity, while C. rhodesianus retained the highest activity. CONCLUSION: A firsthand knowledge of AChE activity in regional Callosobruchus in line with the prevailing food types should be of utmost importance to grain legume breeders, researchers on plant materials for bruchid control and pesticide manufacturer/applicators for a robust integrated management of these bruchids.

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Chitin is an important structural component of the cellular wall of fungi and exoskeleton of many invertebrate plagues, such as insects and nematodes. In digestory systems of insects it forms a named matrix of peritrophic membrane. One of the most studied interaction models protein-carbohydrate is the model that involves chitin-binding proteins. Among the involved characterized domains already in this interaction if they detach the hevein domain (HD), from of Hevea brasiliensis (Rubber tree), the R&R consensus domain (R&R), found in cuticular proteins of insects, and the motif called in this study as conglicinin motif (CD), found in the cristallography structure of the β-conglicinin bounded with GlcNac. These three chitin-binding domains had been used to determine which of them could be involved in silico in the interaction of Canavalia ensiformis and Vigna unguiculata vicilins with chitin, as well as associate these results with the WD50 of these vicilins for Callosobruchus maculatus larvae. The technique of comparative modeling was used for construction of the model 3D of the vicilin of V. unguiculata, that was not found in the data bases. Using the ClustalW program it was gotten localization of these domains in the vicilins primary structure. The domains R&R and CD had been found with bigger homology in the vicilins primary sequences and had been target of interaction studies. Through program GRAMM models of interaction ( dockings ) of the vicilins with GlcNac had been gotten. The results had shown that, through analysis in silico, HD is not part of the vicilins structures, proving the result gotten with the alignment of the primary sequences; the R&R domain, although not to have structural similarity in the vicilins, probably it has a participation in the activity of interaction of these with GlcNac; whereas the CD domain participates directly in the interaction of the vicilins with GlcNac. These results in silico show that the amino acid number, the types and the amount of binding made for the CD motif with GlcNac seem to be directly associates to the deleterious power that these vicilins show for C. maculatus larvae. This can give an initial step in the briefing of as the vicilins interact with alive chitin in and exert its toxic power for insects that possess peritrophic membrane

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Visando avaliar os efeitos de diferentes temperaturas de armazenamento e de genótipos de feijoeiro, Phaseolus vulgaris L., sobre a expressão da resistência ao caruncho, Acanthoscelides obtectus (Say), realizaram-se ensaios com os genótipos Arc.1S, Arc.1, Arc.2, Arc.4 e Carioca Pitoco. Os grãos de Arc.1S e Arc.2 expressaram resistência a A. obtectus nas temperaturas de 20ºC, 25ºC e 30°C; a resistência do genótipo Arc.1 diminuiu à medida que a temperatura de armazenamento foi elevada de 25ºC para 30ºC. A interação genótipos x temperaturas foi significativa, indicando que a elevação da temperatura afeta a expressão da resistência. A temperatura de 20ºC foi a mais adequada para discriminar os genótipos de feijoeiro, quanto a resistência.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Durante o armazenamento de grãos de feijão (Phaseolus vulgaris L.), o rendimento pode ser reduzido devido às infestações de carunchos como os da espécie Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera: Bruchidae). O ataque desse inseto afeta diretamente a qualidade dos grãos, além de facilitar a entrada de patógenos, tornando-os inviáveis para o consumo e/o comércio. Com a finalidade de buscar uma estratégia alternativa para o controle deste caruncho, avaliou-se a possível resistência de linhagens quase isogênicas contendo arcelina, linhagens selvagens contendo arcelina e cultivares comerciais de feijoeiro, em laboratório (T= 25±2° C, U.R.= 70±10% e fotoperíodo= 12h). Foram utilizados frascos contendo 10 g de grãos dos genótipos, os quais foram infestados por uma semana com sete casais do caruncho. Vinte e um dias após a infestação, os grãos foram avaliados contando-se o número de ovos viáveis. A partir de 25 dias da infestação, os grãos foram observados diariamente avaliando-se o número e o peso dos insetos emergidos, a viabilidade larval, o ciclo biológico (ovo-adulto) e o peso de grãos consumidos. Empregou-se um delineamento inteiramente casualizado, com oito repetições. Os genótipos Arc.2, Arc.3, Arc.4, Arc.3S e Ipa 6 expressaram baixos níveis de não-preferência para oviposição e foram classificados como deterrentes. Os genótipos Arc.1S e Arc.1 expressaram elevados níveis de antibiose; Arc.2, Arc.3 e Arc.4 apresentam o mesmo mecanismo, porém, em níveis inferiores.

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During bean seed storage, yield can be lost due to infestations of Acanthoscelides obtectus Say, the bean weevil. The use of resistant varieties has shown promising results in fighting these insects, reducing infestation levels and eliminating chemical residues from the beans. The expression of resistance to A. obtectus in bean varieties is frequently attributed to the presence of phytohemagglutinins, protease inhibitors and alpha-amylase, and especially to variants of the protein arcelin, which reduce the larval viability of these insects. To evaluate the effect of bean seed storage time on the resistance expression of bean varieties to A. obtectus, tests with seeds of three ages (freshly-harvested, 4-month-old, and 8-month-old) were conducted in the laboratory, using four commercial varieties: Carioca Pitoco, Ipa 6, Porrillo 70, Onix; four improved varieties containing arcelin protein: Are. 1, Arc.2, Arc. 3, Arc.4; and three wild varieties also containing arcelin protein: Arc. IS, Arc.3S, and Arc. 5S. The Arc.5S, Arc. IS, and Arc.2 varieties expressed high antibiosis levels against the weevil; Arc. I and Arc3S expressed the same mechanism, but at lower levels. The occurrence of oviposition non-preference was also observed in Arc.5S and Arc. IS. The Arc.3 and Arc. 4 varieties expressed low feeding non-preference levels against A. obtectus. The expression of resistance in arcelin-bearing, wild or improved varieties was affected during the storage of seeds, and was high under some parameters but low in others. The results showed that addition of chemical resistance factors such as protein arcelin via genetic breeding may be beneficial in improving the performance of bean crops.

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This is the first record of Acanthoscelides schrankiae Horn, feeding in seeds of Mimosa bimucronata (DC.) Kuntze. We investigated the pattern of oviposition and seed exploitation by A. schrankiae, and the distribution of mature fruits and seed predation in the inflorescences. We also compared the percentage of predated seeds, the total dry weight of fruits and non-predated seeds, the percentage of aborted seeds, and the percentage of non-emergent insects, among different quadrants of the M. bimucronata canopy. To determine the occurring species, the emergence of bruchids and parasitoids was observed in the laboratory, resulting altogether, only in individuals of A. schrankiae and Horismenus sp. (Hymenoptera: Eulophidae) species, respectively. Mean number of fruits produced in the median region of inflorescence was significantly higher than in the inferior and superior regions, and the frequencies (observed and expected) of predated and non-predated seeds differed among the different regions of inflorescence. Females of A. schrankiae laid their eggs on fruits, and larvae, after emergence, perforated the exocarp to reach the seeds. Most fruits presented one to three eggs and only one bruchid larva was observed in each seed. The highest value of the rate number of eggs/fruit and the highest percentage of predated seeds were recorded in April. Dry weight of fruits (total) and seeds (non-predated), proportions of predated seeds, seed abortions, and non-emergent seed predators, were evenly distributed in the canopy.

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Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The aim of this work was to evaluate the genetics divergence of bean genotypes in relation to the oviposition, feeding and development of Zabrotes subfasciatus, determining the degrees of resistance to the weevil. The genotypes used were: IAC Carioca-Tybata, IAC Fortaleza, IAPAR 81, IAC Carioca-Ete, IAC Galante, IAC Harmonia, IAC Una, IAC Diplomata, BRS Supremo and RAZ 49. Tests were performed in laboratory under controlled humidity, temperature and photophase conditions. In free choice test, 10 g of bean genotypes seeds were distributed in circular openings placed equidistant from each other in aluminum trays, where 70 couples were released. The attractiveness was evaluated 24 hours and seven days after the experiment started, and then the number of eggs was evaluated. In non choice test, 10 g of seeds were used where seven couples of Z. subfasciatus, 24 hours-old, were released, remaining seven days, and after the adults retreat, the total number of eggs, viable and unviable eggs, the number and percentage of emerged adults, weight, longevity and period from egg to adult of males and females, sex ratio, dry mass and dry mass consumed by insect were evaluated. In the genotype IAC Harmonia was observed the lower oviposition; RAZ 49 was the most non preference-type resistant for feeding and/or antibiosis-type resistant; BRS Supremo, IAC Carioca-Ete and IAPAR 81 are no preference for feeding and/or antibiosis-type moderate resistant; IAC Galante is susceptible and the other genotypes are highly susceptible to Z. subfasciatus.