SLC2A2 gene expression in kidney of diabetic rats is regulated by HNF-1 alpha and HNF-3 beta
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
20/10/2012
20/10/2012
2009
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| Resumo |
We hypothesize that, in kidney of diabetic rats, hepatocyte nuclear factors (HNF-1 alpha. and HNF-3 beta) play a critical role in the overexpression of solute carrier 2A2 (SLC2A2) gene. Diabetic rats submitted or not to rapid (up to 12 h) and short-term (1, 4 and 6 days) insulin treatment were investigated. Twofold increase in GLUT2 mRNA was observed in diabetic, accompanied by significant increases in HNF-1 alpha and HNF-3 beta expression and binding activity. Additional 2-fold increase in GLUT2 mRNA and HNF-3 beta expression/activity was observed in 12-h insulin-treated rats. Six-day insulin treatment decreased GLUT2 mRNA and HNF-1 alpha expression and activity to levels of non-diabetic rats, whereas HNF-3 beta decreased to levels of non-insulin-treated diabetic rats. Our results provide evidence for a link between the overexpression of SLC2A2 gene and the transcriptional activity of HNF-1 alpha and HNF-3 beta in kidney of diabetic rats. Furthermore, recovery of SLC2A2 gene after 6-day insulin treatment also involves HNF-1 alpha and HNF-3 beta activity. (C) 2009 Elsevier Ireland Ltd. All rights reserved. Paulo State Foundation[FAPESP02/07384-4] Paulo State Foundation |
| Identificador |
MOLECULAR AND CELLULAR ENDOCRINOLOGY, v.305, n.1/Fev, p.63-70, 2009 0303-7207 http://producao.usp.br/handle/BDPI/28725 10.1016/j.mce.2009.02.014 |
| Idioma(s) |
eng |
| Publicador |
ELSEVIER IRELAND LTD |
| Relação |
Molecular and Cellular Endocrinology |
| Direitos |
restrictedAccess Copyright ELSEVIER IRELAND LTD |
| Palavras-Chave | #Diabetic nephropathy #GLUT2 #Foxa 2 #EMSA #RENAL PROXIMAL TUBULES #GLUCOSE TRANSPORTERS #GLUT2 GENE #MOLECULAR ADAPTATIONS #INSULIN #CELLS #TRANSCRIPTION #DYSFUNCTION #INHIBITOR #MUTATIONS #Cell Biology #Endocrinology & Metabolism |
| Tipo |
article original article publishedVersion |
