The plasmodium receptor for activated C kinase protein inhibits Ca(2+) signaling in mammalian cells
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Plasmodium falciparum, the most lethal malarial parasite, expresses an ortholog for the protein kinase C (PKC) activator RACK1. However, PKC has not been identified in this parasite, and the mammalian RACK1 can interact with the inositol 1,4,5-trisphosphate receptor (InsP3R). Therefore we investigated whether the Plasmodium ortholog PfRACK also can affect InsP3R-mediated Ca(2+) signaling in mammalian cells. GFP-tagged PfRACK and endogenous RACK1 were expressed in a similar distribution within cells. PfRACK inhibited agonist-induced Ca(2+) signals in cells expressing each isoform of the InsP3R, and this effect persisted when expression of endogenous RACK1 was reduced by siRNA. PfRACK also inhibited Ca(2+) signals induced by photorelease of caged InsP3. These findings provide evidence that PfRACK directly inhibits InsP3-mediated Ca(2+) signaling in mammalian cells. Interference with host cell signaling pathways to subvert the host intracellular milieu may be an important mechanism for parasite survival. (C) 2009 Elsevier Inc. All rights reserved. NIH[DK45710] U.S. National Institutes of Health (NIH) U.S. National Institutes of Health (NIH) NIH[DK57751] U.S. National Institutes of Health (NIH) NIH[DK34989] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP |
Identificador |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.389, n.4, p.586-592, 2009 0006-291X http://producao.usp.br/handle/BDPI/27440 10.1016/j.bbrc.2009.09.025 |
Idioma(s) |
eng |
Publicador |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
Relação |
Biochemical and Biophysical Research Communications |
Direitos |
restrictedAccess Copyright ACADEMIC PRESS INC ELSEVIER SCIENCE |
Palavras-Chave | #Plasmodium falciparum #Receptor for activated C kinase #InsP3 receptors #Calcium signaling #Primary hepatocytes #INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR #HUMAN MALARIA PARASITES #PERFUSED-RAT-LIVER #HOST-CELL #TRYPANOSOMA-CRUZI #MAURERS CLEFTS #BILE SECRETION #CALCIUM #FALCIPARUM #RACK1 #Biochemistry & Molecular Biology #Biophysics |
Tipo |
article original article publishedVersion |