Evaluation of susceptibility of pear and plum varieties and rootstocks to Ca. P. pyri and Ca. P. prunorum using Real-Time PCR


Autoria(s): Torres, E.; Laviña Gomila, Amparo; Sabaté, J.; Bech, J.; Batlle i Durany, Assumpció
Contribuinte(s)

IRTA. Recerca i Tecnologia Agroalimentàries

Data(s)

2010

Resumo

Real-time PCR was used to quantify phytoplasma concentration in fifty inoculated trees from five Prunus rootstocks and in forty-eight symptomatic pear and Japanese plum trees from orchards. Seasonal fluctuation of Ca. P. prunorum in different Prunus rootstocks, over three years, showed that the highest percentage detected by nested-PCR was in the ‘Garnem’ rootstock on nearly all sampling dates. Intra-varietal differences were also observed. Phytoplasma titer could be estimated by real time PCR in some trees of the rootstocks ‘Garnem’, ‘Barrier’, ‘GF-677’ and ‘Marianna’, and ranged from 4.7x105 to 3.18x109 phytoplasmas per gram of tissue. Quantification by real-time PCR was not possible in the ‘Cadaman’ trees analyzed, probably due to a lower phytoplasma titer in this variety. Samples from infected trees from commercial plots had different phytoplasma concentration and detection percentage depending on the variety, both being lower in ‘Fortune’ and ‘606’ Japanese plum and in ‘Blanquilla’ pear trees.

Formato

4 p.

Identificador

http://hdl.handle.net/2072/179101

Idioma(s)

eng

Publicador

Julius-Kühn-Archiv

Direitos

info:eu-repo/semantics/openAccess

L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/

Fonte

RECERCAT (Dipòsit de la Recerca de Catalunya)

Palavras-Chave #Fitoplasma #631 - Agricultura. Agronomia. Maquinària agrícola. Sòls. Edafologia agrícola
Tipo

info:eu-repo/semantics/article