Molecular and morphological markers for rapid distinction between 2 Colletotrichum species


Autoria(s): PILEGGI, Sonia Alvim Velga; OLIVEIRA, Sarah Franco Vieira de; ANDRADE, Caroline Waculicz; VICENTE, Vania Aparecida; DALZOTO, Patricia do Rocio; CRUZ, Gerson Kniphoff da; GABARDO, Juarez; MASSOLA JR., Nelson Sidnei; TOZZE JR., Hugo Jose; PILEGGI, Marcos; KAVA-CORDEIRO, Vanessa; GALLI-TERASAWA, Lygia Vitoria; PIMENTEL, Ida Chapaval; STRINGARI, Danyelle; GLIENKE, Chirlei
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2009

Resumo

Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, however, has been a problem in this type of study. Several authors have reported the presence of endophytic fungi from the medicinal plant Maytenus ilicifolia (espinheira-santa) in Brazil that has antimicrobial activity against various pathogens. Therefore, Colletotrichum strains were isolated from M. ilicifolia and identified based on morphology, RAPD markers, sequence data of the internal transcribed spacer regions (ITS-1 and ITS-2), the 5.8S gene, and species-specific PCR. The analyses suggested the presence of 2 species, Colletotrichum gloeosporioides and Colletotrichum boninense. Two morphological markers were characterized to allow C. gloeosporioides and C. boninense to be distinguished quickly and accurately. The molecular diagnosis of C. boninense was confirmed by using Coll and ITS4 primers. This species of Colletotrichum is reported for the first time in M. ilicifolia.

Universidade Estadual de Ponta Grossa (UEPG)

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

Identificador

CANADIAN JOURNAL OF MICROBIOLOGY, v.55, n.9, p.1076-1088, 2009

0008-4166

http://producao.usp.br/handle/BDPI/19121

10.1139/W09-059

http://dx.doi.org/10.1139/W09-059

Idioma(s)

eng

Publicador

NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Relação

Canadian Journal of Microbiology

Direitos

restrictedAccess

Copyright NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Palavras-Chave #Colletotrichum gloeosporioides #Colletotrichum boninense #endophytic fungi #RAPD #ITS #MAYTENUS-ILICIFOLIA #ENDOPHYTIC FUNGUS #DIVERSITY #GLOEOSPORIOIDES #METABOLITES #AQUIFOLIUM #IDENTIFICATION #ANTIBACTERIAL #PRODUCTS #CITRUS #Biochemistry & Molecular Biology #Biotechnology & Applied Microbiology #Immunology #Microbiology
Tipo

article

original article

publishedVersion