Preclinical Studies with Umbilical Cord Mesenchymal Stromal Cells in Different Animal Models for Muscular Dystrophy


Autoria(s): ZUCCONI, Eder; VIEIRA, Natassia Moreira; BUENO JR., Carlos Roberto; SECCO, Mariane; JAZEDJE, Tatiana; VALADARES, Marcos Costa; SUZUKI, Miriam Fussae; BARTOLINI, Paolo; VAINZOF, Mariz; ZATZ, Mayana
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/04/2012

18/04/2012

2011

Resumo

Umbilical cord mesenchymal stromal cells (MSC) have been widely investigated for cell-based therapy studies as an alternative source to bone marrow transplantation. Umbilical cord tissue is a rich source of MSCs with potential to derivate at least muscle, cartilage, fat, and bone cells in vitro. The possibility to replace the defective muscle cells using cell therapy is a promising approach for the treatment of progressive muscular dystrophies (PMDs), independently of the specific gene mutation. Therefore, preclinical studies in different models of muscular dystrophies are of utmost importance. The main objective of the present study is to evaluate if umbilical cord MSCs have the potential to reach and differentiate into muscle cells in vivo in two animal models of PMDs. In order to address this question we injected (1) human umbilical cord tissue (hUCT) MSCs into the caudal vein of SJL mice; (2) hUCT and canine umbilical cord vein (cUCV) MSCs intra-arterially in GRMD dogs. Our results here reported support the safety of the procedure and indicate that the injected cells could engraft in the host muscle in both animal models but could not differentiate into muscle cells. These observations may provide important information aiming future therapy for muscular dystrophies.

Identificador

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2011

1110-7243

http://producao.usp.br/handle/BDPI/15780

10.1155/2011/715251

http://dx.doi.org/10.1155/2011/715251

Idioma(s)

eng

Publicador

HINDAWI PUBLISHING CORPORATION

Relação

Journal of Biomedicine and Biotechnology

Direitos

openAccess

Copyright HINDAWI PUBLISHING CORPORATION

Palavras-Chave #TRANSGENIC MDX MICE #STEM-CELLS #SATELLITE CELLS #NEUROMUSCULAR DISEASES #MIYOSHI MYOPATHY #GENE-THERAPY #FULL-LENGTH #IN-VIVO #MUSCLE #TRANSPLANTATION #Biotechnology & Applied Microbiology #Medicine, Research & Experimental
Tipo

article

original article

publishedVersion