Alcohol dehydrogenase activities and ethanol tolerance in Anastrepha (Diptera, Tephritidae) fruit-fly species and their hybrids


Autoria(s): CARVALHO, Eneas; SOLFERINI, Vera Nisaka; MATIOLI, Sergio Russo
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

26/03/2012

26/03/2012

2009

Resumo

The ADH (alcohol dehydrogenase) system is one of the earliest known models of molecular evolution, and is still the most studied in Drosophila. Herein, we studied this model in the genus Anastrepha (Diptera, Tephritidae). Due to the remarkable advantages it presents, it is possible to cross species with different Adh genotypes and with different phenotype traits related to ethanol tolerance. The two species studied here each have a different number of Adh gene copies, whereby crosses generate polymorphisms in gene number and in composition of the genetic background. We measured certain traits related to ethanol metabolism and tolerance. ADH specific enzyme activity presented gene by environment interactions, and the larval protein content showed an additive pattern of inheritance, whilst ADH enzyme activity per larva presented a complex behavior that may be explained by epistatic effects. Regression models suggest that there are heritable factors acting on ethanol tolerance, which may be related to enzymatic activity of the ADHs and to larval mass, although a pronounced environmental effect on ethanol tolerance was also observed. By using these data, we speculated on the mechanisms of ethanol tolerance and its inheritance as well as of associated traits.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificador

Genetics and Molecular Biology, v.32, n.1, p.177-185, 2009

1415-4757

http://producao.usp.br/handle/BDPI/11598

10.1590/S1415-47572009005000012

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100027

http://www.scielo.br/pdf/gmb/v32n1/2007-276.pdf

Idioma(s)

eng

Publicador

Sociedade Brasileira de Genética

Relação

Genetics and Molecular Biology

Direitos

openAccess

Copyright Sociedade Brasileira de Genética

Palavras-Chave #Alcohol dehydrogenase #Anastrepha #Hybrids #Ethanol tolerance #Tephritidae
Tipo

article

original article

publishedVersion