Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy
Data(s) |
2007
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Resumo |
The hybridization of immobilized oligonucleotides probe strands with solution phase targets is the underlying principle of microarraybased techniques for the analysis of DNA variation. To study the kinetics of DNA/DNA hybridization, target DNA is often prior labeled with markers. A label-free method of electrochemical impedance spectra (EIS) for study the hybridization in process was reported. The Langmuir model was used to determine the association rate constant (K-on), the dissociation rate constant (K-off) and the affinity rate constant (K-A), for perfect matched DNA hybridization. The results show that, EIS is a successful technique possessing high effectivity and sensitivity to study DNA/DNA hybridization kinetics. This work can provide another view on EIS for the studying of DNA/DNA hybridization. |
Identificador | |
Idioma(s) |
英语 |
Fonte |
Li DL;Zou XQ;Shen Q;Dong SJ.Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy,ELECTROCHEMISTRY COMMUNICATIONS,2007 ,9(2):191-196 |
Palavras-Chave | #PLASMON FLUORESCENCE SPECTROSCOPY #DNA HYBRIDIZATION #LABEL-FREE #MONOLAYERS #RESONANCE #SURFACES #DESIGN #SENSOR #GOLD |
Tipo |
期刊论文 |