Saponins, IL12 and BCG adjuvant in the FML-vaccine formulation against murine visceral leishmaniasis

The FML antigen of Leishmania donovani, in combination with either Riedel de Haen (R), QuilA, QS21 saponins, IL12 or BCG, was used in vaccination of an outbred murine model against visceral leishmaniasis (VL). Significant and specific increases in anti-FML IgG and IgM responses were detected for all adjuvants, and in anti-FML IgG1, IgG2a and IgG2b and delayed type of hypersensitivity to L. donovani lysate (DTH), only for all saponins and IL12. The QS21-FML and QuilA-FML groups achieved the highest IgG2a response. QuilA-FML developed the strongest DTH and QS21-FML animals showed the highest serum IFN-gamma concentrations. The reduction of parasitic load in the liver in response to each FML-vaccine formulation was: 52% (P < 0.025) for BCG-FML, 73% (P < 0.005) for R-FML, 93% (P < 0.005) for QuilA-FML and 79.2% (P < 0.025) for QS21-FML treated animals, respectively. Protection was specific for R-FML and QS21-FML while the QuilA saponin treatment itself induced 69% of LDU reduction. The FML-saponin vaccines promote significant, specific and strong protective effects against murine visceral leishmaniasis. BCG-FML induced minor and non-specific protection while IL 12-FML, although enhancing the specific antibody and IDR response, failed to reduce the parasitic load of infected animals. (C) 2002 Elsevier B.V. Ltd. All rights reserved.

Efeito da somatotropina bovina recombinante no desempenho e nas características químicas da carne de bovinos

The objective of this study was to evaluate the effects of the use of recombinant bovine somatotropin (rbST) in weight gain, feeding efficiency and chemical characteristics of meat of young bulls. Fifty nine young bulls were used: 30 crossbred Simmental x Nelore and 29 Nelore aging between seven to eight months, weighing 236 and 116 kg, respectively. A randomized experiment in a 2 × 2 factorial arrangement was used. There were two levels of rbST (0 and 250 mg/animal/14 days) and two breed groups subdivided in two experimental phases. Diet was based on corn silage and concentrate aiming at a weight gain of 0.8 a day for Phase I (70 days) and 1.3 kg for Phase II (98 days). Animals were slaughtered at 13 months of age and the measurements of carcass dressing and meat quality were taken. Results showed that cross-breeding animals overcame (p<.01) the Nelore breed in weight gain and final weight at slaughtering. Use of rbST has not influenced weight gain, final weight, carcass weight and chemical characteristics of meat (p>.05). The use of rbST improved feeding efficiency 11.91 % for cross-bred and 24.32% for Nelore in Phase II.

Papel da eritropoetina recombinante humana sobre o metabolismo dos carboidratos, paratormônio, cálcio iônico, zinco, prolactina e níveis pressóricos em renais crônicos tratados com hemodiálise

Objective: To evaluate the influence of recombinant human erythropoietin (Epho) on carbohydrate metabolism, parathyroid hormone, calcium ionic, zinc, prolactin and blood pressure (BP) in chronic renal failure treated by hemodialysis. Methods: Ten patients in hemodialysis were followed during 24 weeks in two phases: 12 weeks pre-Epho (BP was measured pre and post hemodialysis sessions) and 12 weeks post-Epho (BP was measured as above and also the blood levels of glucose, insulin, parathyroid hormone, calcium ionic, prolactin, and zinc). Results: Patients were 39.8±8.5 y, 50% males. Hematocrit and hemoglobin presented a significant increase four weeks after Epho (22.3±2.3 to 28.1±2.6% and 7.4±0.8 to 9.4±0.9 g/dL, p<0.05). BP (mmHg) and weight pre-Epho: 158±99 and 59±13 (before hemodialysis), 147±96 and 55±13 (after hemo) and post-Epho: 161±100 and 59±13 (before hemo) 155±101 and 56±12 (after hemo) were all not statistically different in any moment. There are also no difference pre and post-Epho in fast glucose (91.8±6.5 and 90.8±6.1 mg/dL, p>0.05), parathyroid hormone (341.4±249.3 and 515.7±310 pg/ mL), calcium ionic (3,66±0.63 and 3.76±0.45 mmol/L), prolactin (males: 327±144.1 and 298.1 ±145.2 μg/mL; females: 666.2±426.6 and 659±395.3 μg/mL) and zinc (median of 0.73 and 0.71 μg\L). Basal insulin was lower after Epho (median of 9.1 to 3.8 μg/mL, p<0.05). Conclusion: These data suggest that recombinant human erythropoietin was effective to improve the anemia and the carbohydrate metabolism in patients with chronic renal failure treated by hemodialysis. © Copyright Moreira Jr. Editors. Todos os direitos reservados.

Uso da suplementação de LH recombinante na estimulação ovariana com protocolos de FSH recombinante e agonistas do GnRH: Uma revisão da literatura

The purpose of this review was to assess the efficacy of recombinant LH (r-LH) supplementation for controlled ovarian stimulation in recombinant FSH (r-FSH) and GnRH-agonist (GnRH-a) protocol for IVF/ICSI cycles. Search strategies included on-line surveys of databases from 1990 to 2006. Four trials fulfilled the inclusion criteria (Lisi et al. 2002, Humaidan et al. 2004, Marrs et al. 2004, Tarlatzis et al. 2006). When the review was carried out advantages were observed for the r-LH supplementation protocol with respect to a fewer days of stimulation, a fewer total amount of r-FSH administered and a higher serum estradiol levels on the day of hCG administration. However, these differences were not observed in number of oocyte retrieved, number of mature oocytes, clinical pregnancy per oocyte retrieval, implantation and miscarriage rates. Nevertheless, more randomized controlled trials are necessary before evidence-based recommendations regarding exogenous r-LH supplementation in ovarian stimulation protocols with r-FSH and GnRH-a for assisted reproduction treatment can be provided.

O emprego de hormônio luteinizante recombinante associado ao hormônio folículo-estimulante recombinante nos protocolos de estimulação ovariana com antagonista do GnRH

The purpose of this investigation was to verify the efficacy of recombinant LH supplementation for controlled ovarian stimulation in GnRH-antagonist protocol for assisted reproductive technologies cycles. Search strategies included on-line surveys of databases from 1990 to 2006. In this review and meta-analysis, the observed advantages for the LH supplementation protocol were a higher serum estradiol levels on the day of hCG administration and a higher number of mature oocytes. However, there were no differences observed in the total amount of r-FSH administered, days of stimulation, number of oocyte retrieved, the clinical pregnancy rate per oocyte retrieval, the implantation rate and miscarriage rate. This result demonstrates that the association of r-LH with r-FSH may prevent any decrease in estradiol after antagonist administration and a significant higher number of mature oocytes was obtained. Nevertheless, additional randomized controlled trials are needed confirm these observations.

Somatotropina bovina recombinante (rbST) e grupos raciais sobre a precocidade reprodutiva de cabras

The objective of this work was to evaluate the effect of rbST and breed group on the reproductive precocity of goat females. Eighteen goat females of Alpine breed and breed groups 1/2 Boer + 1/2 Alpine (1/2 BA) and 3/4 Boer + 1/4 Alpine (3/4 BA) were used, distributed in two homogeneous groups of 9 animals, received treatments: Group 1-0,3 mg of recombinant bovine somatotropin by kg of body weight each 14 days and Group 2 - control and aplication was subcutaneous. Animals from control group received saline solution in same dose and interval. The administration of hormone begins after weaning of animals (approximate 45 days of age). The train of the development of females was made by weighed weekly until reaching the previously definitive weight of 30 kg of live weight (LW). For evaluation of the reproductive performance it was made observations of the signals related to the oestrus. The characteristics evaluated was: Weight gain at born until weaning, weaning at 120 days, 150 and 180 days of age, weight to the oestrus, age to the rutting and the 30 age to kg of LW. It was not observed influence of growth hormone and the breed group for weight to rutting, age to rutting and age to reach 30 kg LW. The use of rbST in growth females did not modify the parameters of sexual precocity.

Biotecnología e industria farmacéutica: desarrollo y producción de interferon natural y recombinante en un laboratorio argentino

Incluye Bibliografía

Estudo da equivalência entre a lectina artin M obtida a partir da semente da jaca e a sua forma recombinante na afinidade por glicanas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito do fator estimulante de colônia de granulócitos recombinante humano (rhG-CSF) sobre o número de leucócitos, plaquetas e sobre a mobilização de células-tronco hematopoéticas CD34+ para o sangue periférico de cães sadios

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de nanopartículas de quitosana para potencial administração nasal da DISBa-01, uma desintegrina recombinante do veneno da serpente Rhinocerophis alternatus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da desnutrição experimental na resposta imune induzida por BCG e DNAhsp65

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dificuldades na obtenção e caracterização de anticorpos monoclonais murinos anti-proteína F recombinante do vírus (RSV) para diagnóstico laboratorial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito do flunixin meglumine, da somatotropina recombinante bovina e/ou da gonadotrofina coriônica humana na redução da mortalidade embrionária em vacas nelore (Bos taurus indicus)

Pós-graduação em Ciência Animal - FMVA

Expressão da proteína S1 recombinante do vírus da bronquite infecciosa em Saccharomyces cerevisiae para aplicação no imunodiagnóstico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão em Escherichia coli de uma proteína recombinante do gene A2 de Leishmania chagasi e utilização desta proteína no diagnóstico da leishmaniose visceral canina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)