Uveítis pediática no infecciosa, una experiencia de una cohorte colombiana

Resumen: •Antecedentes: La uveítis pediátrica no infecciosa tiene el potencial de desencadenar severas complicaciones visuales y su manejo farmacológico convencional está asociado a importantes efectos secundarios. 1 Infliximab (INF) y Adalimumab (ADA), son dos medicamentos biológicos disponibles para el manejo de la uveítis pediátrica refractaria. Se unen específicamente al TNFα y previenen la unión del mismo con los receptores celulares; interacción directamente implicada en el proceso inflamatorio y el subsecuente daño tisular. 2,3 •Métodos: Se realizó estudio de tipo cohorte retrospectiva mediante revisión de historias clínicas de 35 pacientes pediátricos diagnosticados con uveítis durante los años 2009-2015. Se comparó control de la inflamación ocular, tiempo de respuesta y eventos adversos en pacientes tratados con ADA o INF con dosis bajas de Metotrexate vs. Metotrexate (MTX) como única terapia. •Resultados: El 45.7% de la población estudiada correspondía al sexo femenino, cuya edad promedio de inicio de síntomas y de diagnósticos fue de 9 años. El 80% de los casos fueron uveítis idiopáticas, seguido por Vogt-Koyanagi-Harada (8,5%) y AIJ (5,7%). El 91,4% presentó compromiso ocular bilateral y se documentaron 2 casos de ambliopía. El 12,9% de los pacientes que recibieron MTX como tratamiento de primera línea requirieron escalonamiento terapéutico por presentar eventos adversos (Elevación de enzimas hepáticas e intolerancia gastrointestinal (GI)). El tiempo promedio para alcanzar control de la inflamación con MTX fue 9 semanas, y para Adalimumab fue de 8,75 semanas (P: 0,90). Se comparó la capacidad de controlar la inflamación del MTX vs Anti-TNF, y no se observaron diferencias significativas (P: 0.88).

Psychopathology during childhood and adolescence predicts delusional-like experiences in adults : a 21-year birth cohort study

Objective: Community surveys have shown that many otherwise well individuals report delusional-like experiences. The authors examined psychopathology during childhood and adolescence as a predictor of delusional-like experiences in young adulthood. ---------- Method: The authors analyzed prospective data from the Mater-University of Queensland Study of Pregnancy, a birth cohort of 3,617 young adults born between 1981 and 1983. Psychopathology was measured at ages 5 and 14 using the Child Behavior Checklist (CBCL) and at age 14 using the Youth Self-Report (YSR). Delusional-like experiences were measured at age 21 using the Peters Delusional Inventory. The association between childhood and adolescent symptoms and later delusional-like experiences was examined using logistic regression. ---------- Results: High CBCL scores at ages 5 and 14 predicted high levels of delusional-like experiences at age 21 (odds ratios for the highest versus the other quartiles combined were 1.25 and 1.85, respectively). Those with YSR scores in the highest quartile at age 14 were nearly four times as likely to have high levels of delusional-like experiences at age 21 (odds ratio=3.71). Adolescent-onset psychopathology and continuous psychopathology through both childhood and adolescence strongly predicted delusional-like experiences at age 21. Hallucinations at age 14 were significantly associated with delusional-like experiences at age 21. The general pattern of associations persisted when adjusted for previous drug use or the presence of nonaffective psychoses at age 21. ---------- Conclusion: Psychopathology during childhood and adolescence predicts adult delusional-like experiences. Understanding the biological and psychosocial factors that influence this developmental trajectory may provide clues to the pathogenesis of psychotic-like experiences.

Characterisation of enterovirus 71 replication kinetics in human colorectal cell line, HT29

Hand, Foot and Mouth Disease (HFMD), a contagious viral disease that commonly affects infants and children with blisters and flu like symptoms, is caused by a group of enteroviruses such as Enterovirus 71 (EV71) and coxsackievirus A16 (CA16). However some HFMD caused by EV71 may further develop into severe neurological complications such as encephalitis and meningitis. The route of transmission was postulated that the virus transmit from one person to another through direct contact of vesicular fluid or droplet from the infected or via faecal-oral route. To this end, this study utilised a human colorectal adenocarcinoma cell line (HT29) with epithelioid morphology as an in vitro model for the investigation of EV71 replication kinetics. Using qPCR, viral RNA was first detected in HT29 cells as early as 12 h post infection (hpi) while viral protein was first detected at 48 hpi. A significant change in HT29 cells’ morphology was also observed after 48 hpi. Furthermore HT29 cell viability also significantly decreased at 72 hpi. Together, data from this study demonstrated that co-culture of HT29 with EV71 is a useful in vitro model to study the pathogenesis of EV71

Elucidating the host–pathogen interaction between human colorectal cells and invading Enterovirus 71 using transcriptomics profiling

Enterovirus 71 (EV71) is one of the main etiological agents for Hand, Foot and Mouth Disease (HFMD) and has been shown to be associated with severe clinical manifestation. Currently, there is no antiviral therapeutic for the treatment of HFMD patients owing to a lack of understanding of EV71 pathogenesis. This study seeks to elucidate the transcriptomic changes that result from EV71 infection. Human whole genome microarray was employed to monitor changes in genomic profiles between infected and uninfected cells. The results reveal altered expression of human genes involved in critical pathways including the immune response and the stress response. Together, data from this study provide valuable insights into the host–pathogen interaction between human colorectal cells and EV71.

Elucidating the host-pathogen interactions between in vitro human cells and enterovirus 71 in Hand, Foot and Mouth Disease (HFMD)

This study has provided further understanding of the pathogenesis of EV71, one of the major etiological agents associated with significant mortality in Hand, Foot and Mouth disease. Elucidating the host-pathogen interaction and the mechanism that the virus uses to bypass host defence systems to establish infection will aid in the development of potential antiviral therapeutics against EV71.

Droplet digital PCR as a useful tool for the quantitative detection of Enterovirus 71

Hand, foot and mouth disease (HFMD) is a contagious viral disease that frequently affects infants and children and present with blisters and flu-like symptoms. This disease is caused by a group of enteroviruses such as enterovirus 71 (EV71) and coxsackievirus A16 (CA16). However, unlike other HFMD causing enteroviruses, EV71 have also been shown to be associated with more severe clinical manifestation such as aseptic meningitis, brainstem and cerebellar encephalitis which may lead to cardiopulmonary failure and death. Clinically, HFMD caused by EV71 is indistinguishable from other HFMD causing enteroviruses such as CA16. Molecular diagnosis methods such as the use of real-time PCR has been used commonly for the identification of EV71. In this study, two platforms namely the real-time PCR and the droplet digital PCR were compared for the detection quantitation of known EV71 viral copy number. The results reveal accurate and consistent results between the two platforms. In summary, the droplet digital PCR was demonstrated to be a promising technology for the identification and quantitation of EV71 viral copy number.

Bile acids in asymmetric synthesis and molecular recognition

This account summarizes the progress made in our laboratory towards the development of new uses of naturally occurring bile acids. Applications in Asymmetric Synthesis (intramolecular coupling, and intermolecular reactions) and Molecular Recognition are described with suitable examples.

Letras, Nº 71, 2015

Contenido: El azaroso viaje de la hispanidad en nuestra Argentina de hoy / Javier Roberto González -- Viajes en palabras : entre historias de vidas y de culturas / Sofía Carrizo Rueda -- "Fuesse paral Papa et contól la sua fazienda" : la escritura ejemplar del viaje en el Conde Lucanor / Juan Paredes -- La poética del libro de viaje entre la Edad Media y el siglo XXI / Julio Peñate Rivero -- Relatos de viaje y paradigmas culturales / Luis Alburquerque García -- Espacio y desplazamiento en el viaje de Santa Oria / Javier Roberto González -- El inédito Ms. BNM 17.806 y los itinerarios de la Sagrada Escritura : edición y estudio de un texto ignorado / María Mercedes Rodríguez Temperley -- Dilemas del paraíso : viaje, utopía y mundanidad en la literatura árabe / Daniel Del Percio -- El viaje dantesco y su funcionalidad metapoética en El sueño y el Infierno de los enamorados del Marqués de Santillana / Cinthia Hamlin -- La aventura no cumplida : el camino del caballero andante hacia el ocaso / Mónica Nasif -- Benjamín de Tudela y los motivos del viaje : la educación del judío y el encuentro con la propia identidad a través del otro / María Gabriela Pauer -- El motivo del viaje en la literatura de sentencias / Alicia Esther Ramadori -- La travesía del caballero andante : mapas e itinerarios en el Amadis de Gaula / María del Rosario Valenzuela Munguía -- El viaje del héroe en el Libro del caballero Zifar, el caso de Zifar y Roboán / Simón Andrés Villegas -- La dinámica del viaje en el contexto del Ms. Esc. K-III-4 : Libro de Apolonio, Vida de Santa María Egipciaca, Libro de los tres reyes de Oriente -- Reseñas

Sapientia, 1964, Vol. XIX, nº 71 (número completo)

Contenido: Concepto y ser / Octavio N. Derisi – La memoria metafísica según el libro X de las “Confesiones” / Mariano N. Castex – Las cosas bellas / Omar Argerami – El fundamento divino de la esencia y existencia del hombre / Octavio N. Derisi – Notas y comentarios -- Bibliografía

Diário da Constituinte [gravação de vídeo] : [programa n. 617]

A emenda da anistia aos microempresários ganha um novo texto que poderá facilitar o acordo para a votação. O primeiro turno deve acabar na próxima semana. Na sessão de ontem foram aprovados três temas importantes. Uma das emendas aprovadas, com 319 votos, é que todos os incentivos fiscais concedidos pela União, estados e municípios serão revistos e poderão ser extintos caso a lei venha considerar que esses incentivos não sejam mais válidos. Foi aprovada a emenda que retira a obrigatoriedade da contribuição sindical. A contribuição só poderá ser definida entre os sindicatos e os empregados. Foi aprovada uma fusão de emendas estabelecendo uma lei agrícola para o país. Os autores da emenda da anistia aos microempresários fizeram um novo texto para facilitar o acordo e a votação da próxima semana. Será anistiada a correção monetária aos micro e pequenos empresários urbanos que fizeram empréstimo entre 28 de fevereiro de 1986 a 31 de dezembro de 1987 e aos pequenos e médios que conseguiram o crédito nesse mesmo período. São classificados como pequenos empresários aqueles que tiveram como receita anual máxima cinco mil OTNS. Os médios empresários serão classificados se tiveram a receita anual máxima de quinze mil OTNS. O novo texto dez que a anistia será concedida se a liquidação dos débitos for feita no prazo de 180 dias a partir da promulgação da nova constituição. E não será excluído o pagamento do débito inicial, acrescido dos juros e taxas. O presidente da constituinte, Ulysses Guimarães, acredita que na próxima semana aconteça o fim do primeiro turno de votação.

Cruise Report 71-S-1: Bottomfish

(PDF contains 4 pages.)

Cruise Report 71-S-2: Prawns

(PDF contains 4 pages.)