Staphylococcus spp. Na cavidade bucal e na bolsa periodontal de indivíduos com periodontite crônica: presença e sensibilidade aos antibióticos

Staphylococcus are not usually studied in the oral cavity, when this happens, they are considered to belong to transitory microflora. Individuals that present periodontal disease represent possibles reservoirs of these opportunist bacteria in the oral cavity. The use of antibiotics whether for treatment of periodontal disease or due to hospital infections, may predispose the increase of the Staphylococcus spp. in the oral cavity because they easily become resistant to antibiotics, resulting in superinfection. The study was made with 88 patients, minimum age- 25 years old, presenting chronical periodontitis, with, at least, two sites having a probing pocket bigger or equal to 5mm. After anamnese and clinical periodontal examination samples were taken from the periodontal pocket using paper cones and from the oral cavity using mouth rinse. Of the total patients 37,50% presented Staphylococcus spp. in the periodontal pocket and 61,36% in lhe oral cavity; 27,27% presented bacteria in the two sites, not necessarily of the same specie. S. epidermidis was the most prevailing specie in periodontal pocket (15,9%) and oral cavity (27,27%). Positive for S. aureus in the periodontal pocket were 4,5% and for the oral cavity 25%, and 3,4% were positive for the two sites. There was not found significative statistical difference referring to the presence of the microorganisms as to age, smoking habit and increase of the probing depth. The majority of the isolated Staphylococcus samples showed resistance to the tested antibiotics, indicating that the drugs as an adjunct to periodontal therapy, must be seen with caution

Desempenho operacional e energético de colhedoras de cana-de-açúcar ( Saccaharum spp.) para uma e duas linhas da cultura

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Manejo hídrico na produção de brotos e mudas de quatro clones de Eucalyptus spp

Pós-graduação em Ciência Florestal - FCA

Diagnóstico morfológico e molecular de Haemonchus spp. em bovinos e ovinos

Pós-graduação em Biologia Geral e Aplicada - IBB

Immune response of broiler chickens immunized orally with the recombinant proteins flagellin and the subunit B of cholera toxin associated with Lactobacillus spp.

Processo FAPESP: 09/53570-3

Continuous vs. segmented second-dimension system gradients for comprehensive two-dimensional liquid chromatography of sugarcane (Saccharum spp.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Screening of Extracts of Leaves and Stems of Psychotria spp. (Rubiaceae) against Sitophilus zeamais (Coleoptera: Curculionidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae) for Maize Protection

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Serology, isolation, and molecular detection of Leptospira spp. from the tissues and blood of rats captured in a wild animal preservation centre in Brazil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Leptospira spp. infection in sheep herds in southeast Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms

Three culture media (Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating Brucella spp. One hundred lymph nodes from pigs (n = 50) and wild boars (n = 50) with lymphadenitis were collected in slaughterhouses in the State of Sao Paulo and were assessed on these three selective media for Brucella spp. All of the samples were negative for Brucella spp. on the three culture media. On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media. The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than the Brucella agar.

A comparison of microbiological and molecular detection of vaginal Lactobacillus spp. between mares and women

A possible explanation for endometritis in mares is ascendant contamination from the vagina. The presence of Lactobacillus spp. is considered to be important in women for a healthy vaginal environment; however, there are few studies in mares related to the presence of Lactobacillus in the vaginal flora of healthy mares. The present work aims to determine the occurrence of Lactobacillus spp. in the vaginal micro-environment of mares. A total of 35 crossbred multiparous mares, aged between 4 and 12 years, with no history of reproductive problems and with healthy reproductive tracts, were used. Two vaginal swabs were obtained from the mares during estrus for Lactobacillus isolation and PCR evaluation. Ten human female volunteers, aged between 24 and 35 years, sexually active, with no history of gynecological diseases and treatments in the past two years were used. Lactobacillus spp. were isolated from 5.7% of the mares' vaginal samples and from 90% of the women's vaginal samples. Lactobacillus DNA was detected by PCR in 22.9% of the mares' vaginal samples and in all of the vaginal samples from the healthy women. The primers used here were demonstrated to have in silico specificity for the detection of L. equi (AB425924.1), L. pantheris (DQ471798.1) and L. mucosae (DQ471799.1), but they did not anneal on Enterococcus faecalis (EU887827.1) or E. faecium (EU887814.1). In conclusion, this study showed a low occurrence of Lactobacillus spp. in mares, suggesting that this bacterium may not play a fundamental role in the equilibrium of the vaginal micro- environment of normal mares.

The influence of photodynamic therapy parameters on the inactivation of Candida spp: in vitro and in vivo studies

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ability of Salmonella spp. to Produce Biofilm Is Dependent on Temperature and Surface Material

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atratividade para adultos e preferência para oviposição de Bemisia tabaci (Genn.) biótipo B (Hemiptera: Aleyrodidae) em genótipos de Cucurbita spp

The objective was to evaluate the oviposition preference and attractiveness of squash genotypes (Cucurbita spp.) to Bemisia tabaci biotype B. For bioassays, B. tabaci rearing biotype B was kept under greenhouse conditions. Twenty genotypes of Cucurbita spp. ('Alicia AF 9354', 'Aline AF 9353', 'Golden Delight', 'Nova Caravela', 'Menina Morena', 'Novita', 'AF-6741', 'Atlas', 'Barbara 305', 'Menina Brasileira', 'Caserta', 'Itapua 301', 'Tamara', 'Samira', 'Canhao Seca Gigante', 'Exposicao', 'Novita Plus', 'Daiane', 'Formosa', 'Sandy') were evaluated in trials (attractiveness and oviposition choice), under laboratory and greenhouse. The attractiveness (adults/cm(2)) was assessed et 12, 24, 48 and 72 hours after release. After 72 hours leaves were collected for counting the number of eggs (free choice). The area of the sheets were measured to obtain the number of eggs/cm(2) number of adults and / cm(2). The attractiveness index s (AI) and oviposition preference index (OPI) were calculated, and the genotype 'Novita' was adopted as the standard susceptible. The genotype 'Golden Delight' was the most attractive to adults of B. tabaci biotype B. 'Sandy' 'Daiane' and 'Formosa' showed low attractiveness and 'Golden Delight' was the most atractive to B. tabaci biotype B adults. 'Sandy', 'Exposicao', 'Daiane', 'Atlas', 'Tamara' and 'Formosa' expressed no preference for free choise assay. According to the attractiveness index (AI) the genotype 'Golden Delight' was the most attractive to adults of B. tabaci biotype B, and between repellents stood out 'Sandy', 'Daiane', 'Formosa', 'Novita Plus', 'Atlas', 'Barbara 305', 'Canhao Seca Gigante' and 'Menina Brasileira'. The genotypes 'Nova Caravela', 'Golden Delight', 'Aline AF 9353' and 'Samira' are considered by stimulating oviposition preference index (OPI), while the genotypes 'Sandy', 'Exposicao', 'Daiane', 'Atlas', 'Tamara', 'Formosa', 'Menina Morena' and 'Itapua 301' were considered deterrents.

Molecular characterization of fire ants, Solenopsis spp., from Brazil based on analysis of mtDNA gene cytochrome oxidase I

Species from the Solenopsis saevissima (Smith) (Hymenoptera: Formicidae) species group are native to South America and have a cosmopolitan distribution because they have been accidentally introduced in many countries around the world. In Brazil, they have a wide distribution, including urban areas. The present study was conducted to investigate the characterization of Solenopsis genus populations associated with urban/human interference sites in Brazil by analyzing the mitochondrial gene cytochrome oxidase I and estimating the degree of relatedness of these populations to make inferences about their phylogeny and also observe the patterns of mitochondrial haplotype (mitotype) distribution across their range. The results revealed complete geographical coherence and polyphyly for the Solenopsis invicta Buren and Solenopsis saevissima species groups, which confirms the diversity of the genera. It also suggests the possibility that reproductively-isolated populations occur, resulting in the evolutionary process of speciation. No predominant haplotype was found in the populations analyzed, but some were more prevalent.