Evaluation of Three Formulations of Culture Media for Isolation of Brucella spp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
03/12/2014
03/12/2014
01/01/2014
|
Resumo |
Three culture media (Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating Brucella spp. One hundred lymph nodes from pigs (n = 50) and wild boars (n = 50) with lymphadenitis were collected in slaughterhouses in the State of Sao Paulo and were assessed on these three selective media for Brucella spp. All of the samples were negative for Brucella spp. on the three culture media. On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media. The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than the Brucella agar. |
Formato |
3 |
Identificador |
http://dx.doi.org/10.1155/2014/702072 Biomed Research International. New York: Hindawi Publishing Corporation, 3 p., 2014. 2314-6133 http://hdl.handle.net/11449/112348 10.1155/2014/702072 WOS:000335837200001 WOS000335837200001.pdf |
Idioma(s) |
eng |
Publicador |
Hindawi Publishing Corporation |
Relação |
BioMed Research International |
Direitos |
openAccess |
Tipo |
info:eu-repo/semantics/article |