The importance of relative humidity for telenomus remus (Hymenoptera: Platygastridae) parasitism and development on Corcyra cephalonica (Lepidoptera: Pyralidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae) eggs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Taxonomy of Acrobasis larvae and pupae in Eastern North America (Lepidoptera : Pyralidae) /

Mode of access: Internet.

Systematics of immature phycitines (Lepidoptera: Pyralidae) associated with leguminous plants in the southern United States /

Includes index.

Avaliação da ação bioinseticida de SBTI e vicilina de Erythrina velutina em enzimas digestivas e membrana peritrófica de larvas de Plodia interpunctella (Lepidoptera: Pyralidae)

Plodia interpunctella (Indian meal moth) is a cosmopolitan pest that attacks not only a wide range of stored grain as well other food products. Due to its economic importance several researches have focused in a method with ability to control this pest with few or no damage to the environment. The study of digestive enzymes inhibitors, lectins and chitin-binding proteins, has often been proposed as an alternative to reduce insect damage. In this study we report the major classes of digestive enzymes during larval growth in P. Interpunctella, being those proteinases actives at pH 9.5 and optimum temperature of 50 oC to both larvae of the 3rd instar and pre-pupal stage of development. In vitro and zymogram assays presented the effects of several inhibitors, such as SBTI, TLCK and PMSF to intestinal homogenate of 3rd instar larvae of 62%, 92% and 87% of inhibition and In pre-pupal stage of 87%, 62 % and 55% of inhibition, respectively. Zymograms showed inhibition of two low molecular masses protein bands by TLCK and that in presence of SBTI were retarded. These results are indicative of predominance of digestive serine proteinases in gut homogenate from Plodia interpunctella larvae. This serine proteinase was then used as a target to evaluate the effect of SBTI on larvae in in vivo assay. Effect of SBTI on mortality and larval mass was not observed at until 4% of concentration (w/w) in diets. Chitin, another target to insecticidal proteins, was observed by chemical method. Moreover, optic microscopy confirmed the presence of a peritrophic membrane. Established this target, in vivo effect of EvV, a chitin binding vicilin, evaluated during the larval development of P. interpunctella and was obtained a LD50 of 0,23% and WD50 of 0,27% to this protein. Mechanism of action was proposed through of the in vivo digestibility of EvV methodology. During the passage through the larval digestive tract was observed that EvV was susceptible to digestive enzymes and a reactive fragment, visualized by Western blotting, produced by digestion was recovered after dissociation of the peritrophic membrane. The bound of EvV to peritrophic membrane was confirmed by immunohystochemical assays that showed strong immunofluorescent signal of EvV-FITC binding and peritrophic membrane. These results are a indicative that vicilins could be utilized as potential insecticide to Plodia interpunctella and a control methods using EvV as bioinsecticide should be studied to reduce lost caused by storage insect pests

Resistance of Bt transgenic maize to lesser cornstalk borer (Lepidoptera: Pyralidae).

To determine if Bt maize seedlings are protected against lesser cornstalk borer damage, Bt hybrids at the 3 and 4 leaf stages were tested under temperatures between 20-32oC and artificial infestation. A high level of resistance was reported in all Bt maize as expressed by larval survival, larval weight, damage score, and number of surviving plants. All Bt maize protected plants against lesser cornstalk borer damage. Also, the resistance present in the Bt maize was not affected by daytime temperature.

Development and reproduction of Olla v-nigrum (Coleoptera: Coccinellidae) fed Anagasta kuehniella (Lepidoptera: Pyralidae) eggs supplemented with an artificial diet.

2013

Ocorrência de Hypsipyla ferrealis Hampson (LEPIDOPTERA: PYRALIDAE) em andiroba no Estado do Acre.

Relata-se a ocorrência de Hypsipyla ferrealis Hampson no Estado do Acre, infestando sementes de andirobeiras, oriundas do município de Senador Guiomard - AC. No Laboratório de Entomologia da Embrapa Acre, as sementes foram depositadas em bandejas plásticas contendo areia umedecida e acondicionadas no interior de uma gaiola telada. Diariamente, a gaiola foi inspecionada à procura de insetos emergidos, os quais foram identificados como Hypsipyla ferrealis, lepidóptero comumente associado infestando sementes de andirobeira. Este é o primeiro registro desta espécie ocorrendo em sementes de andirobeira no estado do Acre.

Effects of artificial shade on attack by the mahogany shoot borer, Hypsipyla robusta (Moore)

Swietenia macrophylla King (Meliaceae: Swietenioideae) provides one of the premier timbers of the world. The mahogany shoot borer Hypsipyla robusta Moore (Lepidoptera: Pyralidae) is an economically important pest of S. macrophylla throughout Asia, Africa and the Pacific. No viable method of controlling this pest is known. Previous observations have suggested that the presence of overhead shade may reduce attack by H. robusta, but this has not been investigated experimentally. This research was therefore designed to assess the influence of light availability on shoot-borer attack on S. macrophylla, by establishing seedlings under three different artificial shade regimes, then using these seedlings to test oviposition preference of adult moths, neonate larval survival and growth and development of shoot borer larvae. Oviposition preference of shoot borer moths was tested on leaves from seedlings grown under artificial shade for 63 weeks. A significant difference in choice was recorded between treatments, with 27.4 ± 1.5 eggs laid under high shade and 87.1 ± 1.8 under low shade. Neonate larval survival on early flushing leaflets of S. macrophylla did not differ significantly between shade treatments. Larval growth rate, estimated by measuring daily frass width, was significantly higher for those larvae fed on seedlings from the high and medium shade treatments (0.1 mm/day), than the low shade treatment (0.06 mm/day). In laboratory-reared larvae, the total mass of frass produced was significantly higher in the high shade treatment (0.4 g) than under the low shade treatment (0.2 g). Longer tunnel lengths were bored by larvae in plants grown under high shade (12.0 ± 2.4 cm) than under low shade (7.07 ± 1.9 cm). However, pupal mass under low shade was 48% higher than that under the high shade treatment, suggesting that plants grown under high shade were of lower nutritional quality for shoot borer larvae. These results indicate that shading of mahogany seedlings may reduce the incidence of shoot borer attack, by influencing both oviposition and larval development. The establishment of mahogany under suitable shade regimes may therefore provide a basis for controlling shoot borer attack using silvicultural approaches.

Biological control of prickly acacia: Current research and future prospects

Prickly acacia, Vachellia nilotica subsp. indica (syn. Acacia nilotica subsp. indica) (Fabaceae), a major weed in the natural grasslands of western Queensland, has been a target of biological control since the 1980s with limited success to date. Surveys in India, based on genetic and climate matching, identified five insects and two rust pathogens as potential agents. Host-specificity tests were conducted for the insects in India and under quarantine conditions in Australia, and for the rust pathogens under quarantine conditions at CABI in the UK. In no-choice tests, the brown leaf-webber, Phycita sp. A, (Lepidoptera: Pyralidae) completed development on 17 non-target plant species. Though the moth showed a clear preference for prickly acacia in oviposition choice trials screening of additional test-plant species was terminated in view of the potential non-target risk. The scale insect Anomalococcus indicus (Hemiptera: Lecanodiaspididae) developed into mature gravid females on 13 out of 58 non-target plant species tested. In the majority of cases very few female scales matured but development was comparable to that on prickly acacia on four of the non-target species. In multiple choice tests, the scale insect showed a significant preference for the target weed over non-target species tested. In a paired-choice trial under field conditions in India, crawler establishment occurred only on prickly acacia and not on the non-target species tested. Further choice trials are to be conducted under natural field conditions in India. A colony of the green leaf-webber Phycita sp. B has been established in quarantine facilities in Australia and host-specificity testing has commenced. The gall-rust Ravenelia acaciae-arabicae and the leaf-rust Ravenelia evansii (Puccineales: Raveneliaceae) both infected and produced viable urediniospores on Vachellia sutherlandii (Fabaceae), a non-target Australian native plant species. Hence, no further testing with the two rust species was pursued. Inoculation trials using the gall mite Aceria liopeltus (Acari: Eriophyidae) from V. nilotica subsp. kraussiana in South Africa resulted in no gall induction on V. nilotica subsp. indica. Future research will focus on the leaf-weevil Dereodus denticollis (Coleoptera: Curculionidae) and the leaf-beetle Pachnephorus sp. (Coleoptera: Chrysomelidae) under quarantine conditions in Australia. Native range surveys for additional potential biological control agents will also be pursued in northern and western Africa.

Life cycle and host range of Phycitasp. rejected for biological control of prickly acacia in Australia

Prickly acacia (Vachellia nilotica subsp. indica), a native of the Indian subcontinent, is a serious weed of the grazing areas of northern Australia and is a target for classical biological control. Native range surveys in India identified a leaf webber, Phycita sp. (Lepidoptera: Pyralidae) as a prospective biological control agent for prickly acacia. In this study, we report the life cycle and host-specificity test results Phycita sp. and highlight the contradictory results between the no-choice tests in India and Australia and the field host range in India. In no-choice tests in India and Australia, Phycita sp. completed development on two of 11 and 16 of 27 non-target test plant species, respectively. Although Phycita sp. fed and completed development on two non-target test plant species (Vachellia planifrons and V. leucophloea) in no-choice tests in India, there was no evidence of the insect on the two non-target test plant species in the field. Our contention is that oviposition behaviour could be the key mechanism in host selection of Phycita sp., resulting in its incidence only on prickly acacia in India. This is supported by paired oviposition choice tests involving three test plant species (Acacia baileyana, A. mearnsii and A. deanei) in quarantine in Australia, where eggs were laid only on prickly acacia. However, in paired oviposition choice trials, only few eggs were laid, making the results unreliable. Although oviposition choice tests suggest that prickly acacia is the most preferred and natural host, difficulties in conducting choice oviposition tests with fully grown trees under quarantine conditions in Australia and the logistic difficulties of conducting open-field tests with fully grown native Australian plants in India have led to rejection of Phycita sp. as a potential biological control agent for prickly acacia in Australia.

Diversidad de artrópodos asociada a dos sistemas de manejo de plantación de Marango (Moringa oleifera Lam.) en la Finca Santa Rosa, Managua

Debido a pocos estudios sobre los artrópodos asociados a Moringa oleifera Lam., se llevó a cabo una caracterización de la diversidad dinámica poblacional y el rol potencial a nivel de orden familia y especie en dos sistemas de manejo de plantación para producción de semillas. La evaluación se llevó a cabo por un periodo de 12 semanas. La colecta se realizó a través del uso de trampas Pitfall y trampas aéreas en lotes de plantación de Moringa oleifera manejadas como monocultivo convencional; y manejo con enfoque agroecológico. Los parámetros registrados en cada sistema de manejo fueron: abundancia riqueza y, diversidad a partir del índice de Shannon Wiener. Los resultados reportan 1775 especímenes colectados, registrándose menor abundancia en manejo convencional (800 individuos) en comparación con manejo agroecológico (975 individuos). La fluctuación poblacional fue diferente en cada sistema de manejo, dándose un mayor incremento entre Noviembre y Enero en manejo agroecológico. La composición de artrópodos estuvo compuesta por 10 ordenes 55 familias y 73 especies. Los órdenes más representativos fueron Hymenoptera, Diptera, Orthoptera y Coleópeta. Las familias más representativas fueron Formicidae, Muscidae, Acrididae, Chrisopidae, Pyralidae y Blattellidae; reportando mayor índice de diversidad (3.2) en manejo agroecológico. La riqueza de especies fue más alta en manejo agroecológico (69) en comparación con manejo convencional (52). En cuanto al rol potencial de las especies en manejo agroecológico fue identificado 49.2% de defoliadores, 42.02% son benéficos mientras en manejo convencional 51.9% don defoliadores y 40.3 son especies benéficas.

The Foraging Ecology of the Mountain Long-Eared Bat Plecotus macrobullaris Revealed with DNA Mini-Barcodes

10 p.

Expurgo da semente de soja com fosfina e seu efeito na qualidade fisiológica - Série Sementes.

Lasioderma serricorne (Coleoptera: Anobiidae); Oryzaephillus surinamensis (Coleoptera: Silvanidae);Cryptolestes ferrugineus (Coleoptera: Cucujidae); Ephestia kuehniella (Lepidoptera: Pyralidae). Expurgo de sementes: tempo de liberação da fosfina (horas); tempo de exposição das sementes a fosfina (horas);

Listeria monocytogenes em condições de simulação do sistema gastrointestinal e a infecção no modelo de Galleria mellonella

Dissertação de mest., Biologia Molecular e Microbiana, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2011

Interação de genótipos de abobrinha italiana com inseticida no controle da broca das cucurbitáceas Diaphania nitidalis Cr.

A resistência de genótipos de abobrinha italiana Cucurbita pepo L. à broca das cucurbitáceas Diaphania nitidalis Cr. foi avaliada a campo. O genótipo Duda apresentou o maior número de frutos produzidos (33 frutos/parcela), enquanto Lita-186 foi o mais preferido, com a maior percentagem de frutos broqueados (3,6%). Os genótipos AG-202, Lita-186 e Duda, mais o inseticida deltametrina 25 CE foram avaliados. Duda comportou-se novamente como mais produtivo (35 frutos/parcela) e menos danificado (0,35%), confirmando os resultados obtidos no primeiro ensaio; já os genótipos AG-202 e Lita-186 revelaram-se os mais preferidos por D. nitidalis, com maiores índices de frutos danificados (2,7 e 6,3%, respectivamente).