Genomics based biomarker discovery in oral squamous cell carcinoma

 The present thesis showed signaling mechanisms and pathways essential for oral cancer progression through genomics approach. It has identified markers that are of diagnostic, prognostic and therapeutic importance. It has also shown that aspirin is a potential drug in oral cancer treatment.

Mass spectrometry-based proteomic data for cancer diagnosis using interval type-2 fuzzy system

An interval type-2 fuzzy logic system is introduced for cancer diagnosis using mass spectrometry-based proteomic data. The fuzzy system is incorporated with a feature extraction procedure that combines wavelet transform and Wilcoxon ranking test. The proposed feature extraction generates feature sets that serve as inputs to the type-2 fuzzy classifier. Uncertainty, noise and outliers that are common in the proteomic data motivate the use of type-2 fuzzy system. Tabu search is applied for structure learning of the fuzzy classifier. Experiments are performed using two benchmark proteomic datasets for the prediction of ovarian and pancreatic cancer. The dominance of the suggested feature extraction as well as type-2 fuzzy classifier against their competing methods is showcased through experimental results. The proposed approach therefore is helpful to clinicians and practitioners as it can be implemented as a medical decision support system in practice.

Immune biomarkers in the spectrum of childhood noncommunicable diseases

A biomarker is an accurately and reproducibly quantifiable biological characteristic that provides an objective measure of health status or disease. Benefits of biomarkers include identification of therapeutic targets, monitoring of clinical interventions, and development of personalized (or precision) medicine. Challenges to the use of biomarkers include optimizing sample collection, processing and storage, validation, and often the need for sophisticated laboratory and bioinformatics approaches. Biomarkers offer better understanding of disease processes and should benefit the early detection, treatment, and management of multiple noncommunicable diseases (NCDs). This review will consider the utility of biomarkers in patients with allergic and other immune-mediated diseases in childhood. Typically, biomarkers are used currently to provide mechanistic insight or an objective measure of disease severity, with their future role in risk stratification/disease prediction speculative at best. There are many lessons to be learned from the biomarker strategies used for cancer in which biomarkers are in routine clinical use and industry-wide standardized approaches have been developed. Biomarker discovery and validation in children with disease lag behind those in adults; given the early onset and therefore potential lifelong effect of many NCDs, there should be more studies incorporating cohorts of children. Many pediatric biomarkers are at the discovery stage, with a long path to evaluation and clinical implementation. The ultimate challenge will be optimization of prevention strategies that can be implemented in children identified as being at risk of an NCD through the use of biomarkers.

New approaches to gene discovery with animal models of obesity and diabetes

DNA-based approaches to the discovery of genes contributing to the development of type 2 diabetes have not been very successful despite substantial investments of time and money. The multiple gene-gene and gene-environment interactions that influence the development of type 2 diabetes mean that DNA approaches are not the ideal tool for defining the etiology of this complex disease. Gene expression-based technologies may prove to be a more rewarding strategy to identify diabetes candidate genes. There are a number of RNA-based technologies available to identify genes that are differentially expressed in various tissues in type 2 diabetes. These include differential display polymerase chain reaction (ddPCR), suppression subtractive hybridization (SSH), and cDNA microarrays. The power of new technologies to detect differential gene expression is ideally suited to studies utilizing appropriate animal models of human disease. We have shown that the gene expression approach, in combination with an excellent animal model such as the Israeli sand rat (Psammomys obesus), can provide novel genes and pathways that may be important in the disease process and provide novel therapeutic approaches. This paper will describe a new gene discovery, beacon, a novel gene linked with energy intake. As the functional characterization of novel genes discovered in our laboratory using this approach continues, it is anticipated that we will soon be able to compile a definitive list of genes that are important in the development of obesity and type 2 diabetes.

Museum and performing arts marketing: the age of discovery

Trends in museum and performing arts marketing from 1975 to 1994 were analyzed and suggested that a third period was emerging; the data in this article confirm that claim. Among the latest arts marketing articles, there is a significantly greater focus on marketing strategy than on the other two categories--marketing as culture and marketing as tactics.

Obesity and diabetes gene discovery approaches

New treatments are currently required for the common metabolic diseases obesity and type 2 diabetes. The identification of physiological and  biochemical factors that underlie the metabolic disturbances observed in obesity and type 2 diabetes is a key step in developing better therapeutic outcomes. The discovery of new genes and pathways involved in the  pathogenesis of these diseases is critical to this process, however  identification of genes that contribute to the risk of developing these diseases represents a significant challenge as obesity and type 2 diabetes are complex diseases with many genetic and environmental causes. A number of diverse approaches have been used to discover and validate potential new targets for obesity and diabetes. To date, DNA-based approaches using candidate gene and genome-wide linkage analysis have had limited success in identifying genomic regions or genes involved in the development of these diseases. Recent advances in the ability to evaluate linkage analysis data from large family pedigrees using variance components based linkage analysis show great promise in robustly identifying genomic regions associated with the development of obesity and diabetes. RNA-based technologies such as cDNA microarrays have identified many genes differentially expressed in tissues of healthy and diseased subjects. Using a combined approach, we are endeavouring to focus attention on differentially expressed genes located in chromosomal regions previously linked with obesity and / or diabetes. Using this strategy, we have identified Beacon as a potential new target for obesity and diabetes.

Accelerating linear causal model discovery using hoeffding bounds

Efficiently inducing precise causal models accurately reflecting given data sets is the ultimate goal of causal discovery. The algorithms proposed by Dai et al. has demonstrated the ability of the Minimum Message Length (MML) principle in discovering Linear Causal Models from training data. In order to further explore ways to improve efficiency, this paper incorporates the Hoeffding Bounds into the learning process. At each step of causal discovery, if a small number of data items is enough to distinguish the better model from the rest, the computation cost will be reduced by ignoring the other data items. Experiments with data set from related benchmark models indicate that the new algorithm achieves speedup over previous work in terms of learning efficiency while preserving the discovery accuracy.

Ensembling MML causal discovery

This paper presents an ensemble MML approach for the discovery of causal models. The component learners are formed based on the MML causal induction methods. Six different ensemble causal induction algorithms are proposed. Our experiential results reveal that (1) the ensemble MML causal induction approach has achieved an improved result compared with any single learner in terms of learning accuracy and correctness; (2) Among all the ensemble causal induction algorithms examined, the weighted voting without seeding algorithm outperforms all the rest; (3) It seems that the ensembled CI algorithms could alleviate the local minimum problem. The only drawback of this method is that the time complexity is increased by δ times, where δ is the ensemble size.

Enhancing UDDI for grid service discovery by using dynamic parameters

A major problem for a grid user is the discovery of currently available services. With large number of services, it is beneficial for a user to be able to discover the services that most closely match their requirements. This report shows how to extend some concepts of UDDI such that they are suitable for dynamic parameter based discovery of grid services.

Toward self discovery for an autonomic cluster

Nondedicated clusters are currently at the forefront of the development of high performance computing systems. These clusters are relatively intolerant of hardware failures and cannot manage dynamic cluster membership efficiently. This report presents the logical design of an innovative self discovery service that provides for automated cluster management and resource discovery. The proposed service has an ability to share or recover unused computing resources, and to adapt to transient conditions autonomically, as well as the capability of providing dynamically scalable virtual computers on demand.

Further pruning for efficient association rule discovery

The Apriori algorithm’s frequent itemset approach has become the standard approach to discovering association rules. However, the computation requirements of the frequent itemset approach are infeasible for dense data and the approach is unable to discover infrequent associations. OPUS AR is an efficient algorithm for association rule discovery that does not utilize frequent itemsets and hence avoids these problems. It can reduce search time by using additional constraints on the search space as well as constraints on itemset frequency. However, the effectiveness of the pruning rules used during search will determine the efficiency of its search. This paper presents and analyses pruning rules for use with OPUS AR. We demonstrate that application of OPUS AR is feasible for a number of datasets for which application of the frequent itemset approach is infeasible and that the new pruning rules can reduce compute time by more than 40%.

Pattern discovery in probabilistic databases

Modeling probabilistic data is one of important issues in databases due to the fact that data is often uncertainty in real-world applications. So, it is necessary to identify potentially useful patterns in probabilistic databases. Because probabilistic data in 1NF relations is redundant, previous mining techniques don’t work well on probabilistic databases. For this reason, this paper proposes a new model for mining probabilistic databases. A partition is thus developed for preprocessing probabilistic data in a probabilistic databases. We evaluated the proposed technique, and the experimental results demonstrate that our approach is effective and efficient.