Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn(1) mutation of the NORK gene in Medicago sativa MN1008
Data(s) |
01/01/2006
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Resumo |
Strategies to introduce genes into non-embryogenic plants for complementation of a mutation are described and tested on tetraploid alfalfa (Medicago sativa). Genes conditioning embryogenic potential, a mutant phenotype, and a gene to complement the mutation can be combined using several different crossing and selection steps. In the successful strategy used here, the M. sativa genotype MnNC-1008(NN) carrying the recessive non-nodulating mutant allele nn(1) was crossed with the highly embryogenic alfalfa line Regen S and embryogenic hybrid individuals were identified from the F1 progeny. After transformation of these hybrids with the wild-type gene (NORK), an F2 generation segregating for the mutation and transgene were produced. Plants homozygous for the mutant allele and carrying the wild-type NORK transgene could form root nodules after inoculation with Sinorhizobium meliloti demonstrating successful complementation of the nn(1) mutation. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Springer |
Palavras-Chave | #Medicago #Stable Transformation #Embryogenicity #Nodulation #Mutant #Complementation #Plant Sciences #Somatic Embryogenesis #Non-nodulation #Ineffective Nodulation #Rhizobium-meliloti #Nodule Development #Root Hairs #Alfalfa #Construction #Registration #Inheritance #C1 #270803 Transgenesis #620108 Grain legumes |
Tipo |
Journal Article |