Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources


Autoria(s): otero, Walter Guimarães; Marino, Carolina Tobias; Barreto, Cristine Chaves; Pellizari, Vivian Helena; Millen, Danilo Domingues; Pacheco, Rodrigo Dias Lauritano; Ferreira, Fernanda Altieri; Dicostanzo, Alfredo; Arrigoni, Mario de Beni; Rodrigues, Paulo Henrique Mazza
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

01/04/2012

Resumo

Nine ruminally cannulated cows fed different energy sources were used to evaluate an avianderived polyclonal antibody preparation against specific ruminal bacteria and monensin on microbial community diversity. The experimental design was three Latin squares 3 x 3 distinguished by the main energy source in the diet [dry-ground corn grain, high moisture corn silage or citrus pulp]. Inside each Latin square, animals received one of the feed additives per period [control, monensin or polyclonal antibody preparation]. Each period lasted 21 days where 20 were used for treatments adaptation and the last one for sampling collection. Microbial diversity was evaluated by protozoa counts and denaturing gradient gel electrophoresis. Polyclonal antibodies plus citrus pulp (CiPu) addition in the diet resulted in an increase of relative counting of Isotricha protozoa that indicates a possible effect on this ruminal ciliate population. In general lines, in the present experiment, it was not possible to assign that there was a pattern in the structures of amplification of Bacteria and Archaea communities of the ruminal content. Oral passive immunization is a technology that arises as an effective alternative for feed additive production. Further research is still necessary to better understand its mechanisms of action.

Formato

491-502

Identificador

http://revistas.ufba.br/index.php/rbspa/article/viewArticle/2185

Revista Brasileira de Saude e Producao Animal, v. 13, n. 2, p. 491-502, 2012.

1519-9940

http://hdl.handle.net/11449/73266

2-s2.0-84865713833

2-s2.0-84865713833.pdf

Idioma(s)

eng

Relação

Revista Brasileira de Saúde e Produção Animal

Direitos

openAccess

Palavras-Chave #Denaturing gradient gel by electrophoresis #Feed additive #Ionophores #Passive immunity #Protozoa #Animalia #Bacteria (microorganisms) #Ciliophora #Citrus #Isotricha #Zea mays
Tipo

info:eu-repo/semantics/article