9 resultados para tlh
Resumo:
Vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Nineteen isolates were confirmed by PCR as V. parahaemolyticus. The tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. Each isolate was resistant to at least one of the nine antimicrobials tested. Additionally, all isolates possessed the blaTEM-116 gene. The presence of this gene in V. parahaemolyticus indicates the possibility of spreading this gene in the environment. Atypical strains of V. parahaemolyticus were also detected in this study.
Resumo:
Vibrio parahaemolyticus is a potentially pathogenic bacterium that occurs naturally in estuarine environments worldwide, and is often associated with gastroenteritis in humans following consumption of raw bivalve mollusks, especially raw oysters. The occurrence of total and pathogenic V. parahaemolyticus in 74 samples of raw oysters collected in restaurants, supermarkets, groceries and beach huts in Sao Paulo State, was monitored between February 2006 and January 2007. Enumeration of V. parahaemolyticus was performed according to the most probable number (MPN) procedure. Five to ten typical colonies were selected from thiosulfate-citrate-bile salts-sucrose (TCBS) agar plates for confirmation by the presence of the species-specific gene tlh and the virulence genes tdh and trh by multiplex PCR. V. parahaemolyticus was detected in 100% of samples. The densities of total V. parahaemolyticus varied from 1.78 to 6.04 logio (MPN/g), with higher densities being detected in fall and summer, and lower densities in winter (P < 0.05). There was no statistical difference among densities of V parahaemolyticus regarding the site of collection. None of the 1943 V parahaemolyticus isolates contained tdh and/or trh. These data provide information for the assessment of exposure to V. parahaemolyticus in oysters consumed in Sao Paulo, State, Brazil. (C) 2010 Elsevier Ltd. All rights reserved.
Resumo:
Vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) encoded by tdh and trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters and mussels were analyzed by PCR using specific primers for the 16S rRNA and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and PFGE. Nineteen isolates were confirmed by PCR as V. parahaemolyticus. The tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. Each isolate was resistant to at least one of the nine antimicrobials tested. Additionally, all isolates possessed the blaTEM-116 gene. The presence of this gene in V. parahaemolyticus indicates the possibility of spreading this gene in the environment. Atypical strains of V. parahaemolyticus were also detected in this study.
Resumo:
Total laparoscopic hysterectomy: impact of body mass index on outcomes
Resumo:
El objetivo general de este proyecto se centra en el estudio, desarrollo y experimentación de diferentes técnicas y sistemas basados en Tecnologías del Lenguaje Humano (TLH) para el desarrollo de la próxima generación de sistemas de procesamiento inteligente de la información digital (modelado, recuperación, tratamiento, comprensión y descubrimiento) afrontando los actuales retos de la comunicación digital. En este nuevo escenario, los sistemas deben incorporar capacidades de razonamiento que descubrirán la subjetividad de la información en todos sus contextos (espacial, temporal y emocional) analizando las diferentes dimensiones de uso (multilingualidad, multimodalidad y registro).
Resumo:
El objetivo de este proyecto se basa en la necesidad de replantearse la filosofía clásica del TLH para adecuarse tanto a las fuentes disponibles actualmente (datos no estructurados con multi-modalidad, multi-lingualidad y diferentes grados de formalidad) como a las necesidades reales de los usuarios finales. Para conseguir este objetivo es necesario integrar tanto la comprensión como la generación del lenguaje humano en un modelo único (modelo LEGOLANG) basado en técnicas de deconstrucción de la lengua, independiente de su aplicación final y de la variante de lenguaje humano elegida para expresar el conocimiento.
Resumo:
El proyecto ATTOS centra su actividad en el estudio y desarrollo de técnicas de análisis de opiniones, enfocado a proporcionar toda la información necesaria para que una empresa o una institución pueda tomar decisiones estratégicas en función a la imagen que la sociedad tiene sobre esa empresa, producto o servicio. El objetivo último del proyecto es la interpretación automática de estas opiniones, posibilitando así su posterior explotación. Para ello se estudian parámetros tales como la intensidad de la opinión, ubicación geográfica y perfil de usuario, entre otros factores, para facilitar la toma de decisiones. El objetivo general del proyecto se centra en el estudio, desarrollo y experimentación de técnicas, recursos y sistemas basados en Tecnologías del Lenguaje Humano (TLH), para conformar una plataforma de monitorización de la Web 2.0 que genere información sobre tendencias de opinión relacionadas con un tema.
Resumo:
La gran cantidad de información disponible en Internet está dificultando cada vez más que los usuarios puedan digerir toda esa información, siendo actualmente casi impensable sin la ayuda de herramientas basadas en las Tecnologías del Lenguaje Humano (TLH), como pueden ser los recuperadores de información o resumidores automáticos. El interés de este proyecto emergente (y por tanto, su objetivo principal) viene motivado precisamente por la necesidad de definir y crear un marco tecnológico basado en TLH, capaz de procesar y anotar semánticamente la información, así como permitir la generación de información de forma automática, flexibilizando el tipo de información a presentar y adaptándola a las necesidades de los usuarios. En este artículo se proporciona una visión general de este proyecto, centrándonos en la arquitectura propuesta y el estado actual del mismo.
Resumo:
Background: There is limited information available on the requirement for postoperative analgesic drugs in patients submitted to total laparoscopic hysterectomy (TLH) compared with patients undergoing vaginal hysterectomy (VH). Aim: To compare the postoperative analgesic requirements in patients who underwent a TLH with patients who had a VH. Methods: Chart review of 53 patients who had TLH and 47 who had VH and were seen postoperatively by an acute pain management service in order to assess postoperative analgesic requirements. Patient controlled analgesia (PCA) was part of the standard protocol for postoperative pain management. Analgesic requirement was recorded as the mean doses of morphine and number of days that patients used non-steroidal anti-inflammatory drugs (NSAIDs), oxycodone and tramadol. Results: The requirement for total morphine was approximately half the dose in patients who had a TLH (10.8 +/- 12.6 mg) compared with patients who had a VH (19.4 +/- 21.9 mg) (P 0.017). The length of use of NSAIDs was significantly reduced in patients who had undergone a TLH (2.0 +/- 0.95 days) as compared with patients who had a VH (2.85 +/- 1.1 days) (P < 0.0001). Conclusions: Patients submitted to TLH require less postoperative analgesic drugs when compared with patients who had VH. Prospective randomised trials are warranted to compare analgesic requirements between patients submitted to TLH and VH.
