Seasonal dynamics of a drosophilid (Diptera) assemblage and its potencial as bioindicator in open environments

Drosophila Fallen, 1823 (Diptera, Drosophilidae) is for long a well-established model organism for genetics and evolutionary research. The ecology of these flies, however, has only recently been better studied. Recent papers show that Drosophila assemblies can be used as bioindicators of forested environment degradation. In this work the bioindicator potential of drosophilids was evaluated in a naturally opened environment, a coastal strand-forest (restinga). Data from nine consecutive seasonal collections revealed strong temporal fluctuation pattern of the majority of Drosophila species groups. Drosophila willistoni group was more abundant at autumns, whereas D. cardini and D. tripunctata groups were, respectively, expressive at winters and springs, and D. repleta group at both seasons. The exotic species D. simulans Sturtevant, 1919 (from D. melanogaster group) and Zaprionus indianus Gupta, 1970 were most abundant at summers. Overall, the assemblage structure did not show the same characteristics of forested or urban environments, but was similar to the forests at winters and to cities at summers. This raises the question that this locality may already been under urbanization impact. Also, this can be interpreted as an easily invaded site for exotic species, what might lead to biotic homogenization and therefore can put in check the usage of drosophilid assemblages as bioindicators at open environments.

Seasonal dynamics of a drosophilid (Diptera) assemblage and its potencial as bioindicator in open environments

Drosophila Fallen, 1823 (Diptera, Drosophilidae) is for long a well-established model organism for genetics and evolutionary research. The ecology of these flies, however, has only recently been better studied. Recent papers show that Drosophila assemblies can be used as bioindicators of forested environment degradation. In this work the bioindicator potential of drosophilids was evaluated in a naturally opened environment, a coastal strand-forest (restinga). Data from nine consecutive seasonal collections revealed strong temporal fluctuation pattern of the majority of Drosophila species groups. Drosophila willistoni group was more abundant at autumns, whereas D. cardini and D. tripunctata groups were, respectively, expressive at winters and springs, and D. repleta group at both seasons. The exotic species D. simulans Sturtevant, 1919 (from D. melanogaster group) and Zaprionus indianus Gupta, 1970 were most abundant at summers. Overall, the assemblage structure did not show the same characteristics of forested or urban environments, but was similar to the forests at winters and to cities at summers. This raises the question that this locality may already been under urbanization impact. Also, this can be interpreted as an easily invaded site for exotic species, what might lead to biotic homogenization and therefore can put in check the usage of drosophilid assemblages as bioindicators at open environments.

Ochlerotatus albifasciatus in rain pools of Buenos Aires: seasonal dynamics and relation to environmental variables

The immature stages of Ochlerotatus albifasciatus develop in temporary pools. The present study aims at evaluating the seasonal dynamics of the aquatic stages of this mosquito, also analyzing the relationship among their presence and breeding success to some relevant climatic and environmental variables in the ephemeral rain pools of an urban park. Nineteen cohorts of O. albifasciatus that developed synchronously after rain events were recorded in all seasons. The proportions of mosquito-positive pools were significantly higher during the fall-winter period than in the spring-summer months (p < 0.001). The presence of this mosquito species was positively related to the amount of rain (p < 0.001), whereas negatively correlated to air temperature (p < 0.05) within a 5.2 to 29.7ºC range. The distribution of the number of cohorts per pool throughout the year was grouped (variance/mean: 3.96), indicating that these habitats were not equally suitable as breeding sites. The immature stages of O. albifasciatus were detected in pools belonging to all of the categories of surface area, depth, duration, vegetation cover, and insolation. However, the proportion of pools where immature mosquitoes were detected was positively and significantly related to surface, depth, duration, and vegetation cover. On the other hand, the proportion of mosquito-positive pools was higher at an intermediate insolation degree. Our results suggest that although preimaginal stages were present in all seasons, high temperatures may be unfavorable to larval development, and substrate vegetation may regulate water temperature. The positive relationship between the proportion of mosquito-positive pools and pool size and duration might reflect a strategy of O. albifasciatus to accomplish immature development.

Seasonal dynamics of Aedes aegypti (Diptera: Culicidae) in the northernmost state of Brazil: a likely port-of-entry for dengue virus 4

Roraima is the northernmost state of Brazil, bordering both Venezuela and Guyana. Appropriate climate and vector conditions for dengue transmission together with its proximity to countries where all four dengue serotypes circulate make this state, particularly the capital Boa Vista, strategically important for dengue surveillance in Brazil. Nonetheless, few studies have addressed the population dynamics of Aedes aegypti in Boa Vista. In this study, we report temporal and spatial variations in Ae. aegypti population density using ovitraps in two highly populated neighbourhoods; Centro and Tancredo Neves. In three out of six surveys, Ae. aegypti was present in more than 80% of the sites visited. High presence levels of this mosquito suggest ubiquitous human exposure to the vector, at least during part of the year. The highest infestation rates occurred during the peak of the rainy seasons, but a large presence was also observed during the early dry season (although with more variation among years). Spatial distribution of positive houses changed from a sparse and local pattern to a very dense pattern during the dry-wet season transition. These results suggest that the risk of dengue transmission and the potential for the new serotype invasions are high for most of the year.

Seasonal dynamics and net production of disolved organic carbon in an oligotrophic coastal environment

To understand dissolved organic carbon (DOC) seasonal dynamics in a coastal oligotrophic site in the north-western Mediterranean Sea, we monitored DOC concentrations monthly over 3 yr, together with the meteorological data and the food-web-related biological processes involved in DOC dynamics. Additional DOC samples were taken in several inshore−offshore transects along the Catalan coast. We found DOC concentrations of ~60 µmol C l−1 in winter, with increasing values through the summer and autumn and reaching 100 to 120 µmol C l−1 in November. There was high inter-annual variability in this summer DOC accumulation, with values of 36, 69 and 13 µmol C l−1 for 2006, 2007 and 2008, respectively. The analysis of the microbial food-web processes involved in the DOC balance did not reveal the causes of this accumulation, since the only occasion on which we observed net DOC production (0.3 ± 1 µmol C l−1 d−1 on average) was in 2007, and the negative DOC balance of 2006 and 2008 did not prevent DOC accumulating. The DOC accumulation episodes coincided with low rates of water renewal (average 0.037 ± 0.021 d−1 from May to October) compared with those of winter to early spring (average 0.11 ± 0.048 d−1 from November to April). Indeed, the amount of DOC accumulated each year was inversely correlated with the average summer rainfall. We hypothesize that decreased DOC turn-over due to photochemical or biological processes mostly active during the summer and low water renewal rate combine to determine seasonal DOC accumulation and influence its inter-annual variability.

Effect of Seasonal Dynamics on Queen Densities of the Argentine Ant (Linepithema Humile) in an Invaded Natural Area of the NE Iberian Peninsula

The annual elimination of large numbers of Argentine ant queens near the advance front of an invasion could be a useful tool for weakening the species’ dispersion and, therefore, limiting its establishment in non-invaded areas. However, before carrying out trials to test the effectiveness of this method it would be essential to have sufficient knowledge of the effect of seasonal dynamics acting on the queens’ densities of the species in order to determine the most favourable period of the year to act. We analyzed the seasonal densities and nest dynamics of Argentine ant queens in an invaded Mediterranean natural ecosystem. We observed that the queens’ density varied depending on the season of the year and that this variation was mainly due to the seasonal dynamics of nest aggregations in winter and ant dispersions in summer. The greatest densities per litre of nest soil were observed in winter (December to March, approximately) and the lowest densities were observed in summer ( June to July). This information is essential for improving current knowledge of the Argentine ant’s biology and developing control methods based on the elimination of queens in invaded natural areas

Evaluation of seasonal dynamics and bioindication potential of macroalgal communities in a polluted tropical stream

Macroalgal communities and environmental variables were analyzed seasonally along a polluted tropical stream in São Paulo State, Brazil, We aimed to improve the knowledge of tropical macroalgal communities, to study the longitudinal and seasonal dynamics of these communities in influenced environments, to evaluate the relationship of algal frequency and abundance with selected environmental variables, and to look for potential macroalgal species indicators. The seasonal abundance pattern was similar to natural or low influenced systems and was mainly determined by rainfall regime. However, community composition was closely related to some chemical water pollution indicators, such as phosphorus and nitrogen compounds. Some species are suggested as potential indicators of organic pollution (e.g. Oscillatoria ornata, Stigeoclonium helveticum and Schizomeris leibleinii), but more detailed studies have to be developed to determine the tolerance limits of these species. on the basis of our results and literature data, the use of occurrence of Oscillatoriales in relation to other orders of Cyanoprokaryota is suggested to be a good indicator of organic pollution in tropical lotic ecosystems.

DISTRIBUTION AND SEASONAL DYNAMICS OF RHODOPHYTA IN THE PRETO RIVER BASIN, SOUTHEASTERN BRAZIL

Distribution and seasonal dynamics of freshwater Rhodophyta were investigated in the Preto River basin, located in northwestern São Paulo State, southeastern Brazil. Twenty-two sites were sampled, six monthly during one year, four bimonthly during two years, and 12 twice (hot-rainy and cool-dry seasons) during the study period (May 1989 through March 1991). Red algal representatives were found in 19 sites (86.4%). Four species occurred in the basin with varying frequencies: Batrachospermum delicatulum (54.5%), Compsopogon coeruleus (36.4%), B. bicudoi (13.6%) and B. virgatum (4.5%). In addition, 'Chantransia' stage of the batrachospermalean species was found in 17 sites (77.3%). B. bicudoi, B. virgatum and C. coeruleus occurred only in large stream segments (greater-than-or-equal-to 3-order), generally in the main river, whereas B. delicatulum was more frequent in small streams (less-than-or-equal-to 3-order). The stream variables most closely related to the species distribution in the basin were specific conductance, pH and oxygen. B. bicudoi and B. delicatulum showed a marked seasonality: gametophytes were observed from late fall to early spring, while the 'Chantransia' stage generally occurred throughout the year. C. coeruleus was observed throughout the year in most sites, but the populations were generally more abundant from late fall to early spring. The combination of lower temperature and reduced turbidity resulting in increased illumination to the benthic algae during the dry winter months promotes the gametophytic growth of the batrachospermalean species, whereas current velocity was found to be the most influential stream variable for C. coeruleus. The persistance of the 'Chantransia' stage throughout the year as well as its tolerance to wider environmental conditions are key factors in the efficiency of the batrachospermalean life history strategy in lotic ecosystems.

SEASONAL DYNAMICS OF MACROALGAL COMMUNITIES IN THE PRETO RIVER BASIN, SAO-PAULO, SOUTHEASTERN BRAZIL

Macroalgal seasonality was analyzed in nine stream segments of the Preto River basin: five of these were sampled monthly for one year and four bimonthly during two years. Seasonal variation of macroalgae was correlated with stream variables (temperature, current velocity, specific conductance, turbidity, surface light, pH and oxygen). Multiple linear regression was applied to evaluate the macroalgal seasonal variability determined by a combination of temperature, current velocity and turbidity, the most influential environmental parameters on macroalgal seasonality. Most species revealed a clear seasonal trend, such that late fall through early spring was the period with highest macroalgal abundance in most sites. Temperature, current velocity and turbidity account for 24 to 83 % of macroalgal species number variation and 24 to 79 % of macroalgal abundance in the basin. The results suggest that the seasonal flow determined by the precipitation regime associated with turbidity and temperature were the major factors influencing the seasonal dynamics of macroalgal communities. The highest values of macroalgal species number and abundance were found under cooler temperatures, lower current velocities and lower values of turbidity.

Community Structure and Seasonal Dynamics of Dactylogyrus spp. (Monogenea) on the Fathead Minnow (Pimephales promelas) from the Salt Valley Watershed, Lancaster County, Nebraska

The gill monogene communities of Pimephales promelas (fathead minnow) in three distinct sites on converging streams were investigated from 2004 to 2006 in three different seasons. Thirty collections of P. promelas were made in southeastern Nebraska along three converging tributaries: Elk Creek (40.88534°N, 96.83366°W), West Oak Creek (40.9082°N, 96.81432°W), and Oak Creek (40.91402°N, 96.770583°W), Lancaster County, Nebraska. In all, 103 P. promelas were collected from Elk Creek, 115 from West Oak Creek, and 78 from Oak Creek and examined for gill monogenes. Among the P. promelas collected, 93.5% were infected with up to three species of Dactylogyrus, including Dactylogyrus simplex Mizelle, 1937, Dactylogyrus bychowskyi Mizelle, 1937, and Dactylogyrus pectenatus Mayes, 1977. Mean intensities at Elk Creek, West Oak Creek, and Oak Creek were 17.6, 22.8, and 25.1, and prevalences 88, 95, and 97%, respectively. At these three sites: (1) P. promelas does not share Dactylogyrus species with Semotilus atromaculatus (creek chub) or Notropis stramineus (sand shiner); (2) fish size and sex are not predictive of Dactylogyrus infection; (3) Dactylogyrus spp. vary (not always predictably) in their seasonal occurrence; (4) populations of Dactylogyrus spp. respond to environmental differences among sites; and (5) the community structure of Dactylogyrus spp. (order of abundance) is independent of environment.

Seasonal dynamics of cyanobacteria in a eutrophic reservoir (Arcoverde) in a semi-arid region of Brazil

Environmental conditions favor the predominance of dense populations of cyanobacteria in reservoirs in northeastern Brazil. The aim of this study was to understand cyanobacterial population dynamics in the rainy and dry seasons at two depths in the Arcoverde reservoir. Microalgae and cyanobacteria samples were collected during 24 hours with intervals of 4 hours (nycthemeral) at sub-surface and 10 m using a van Dorn bottle and a determined biomass. Physical and chemical variables were obtained and the data were analyzed using the principal component analysis (PCA). No nycthemeral variations in the taxonomic composition or distribution of the populations of cyanobacteria were found between the different times of day in either the rainy or dry season. In both seasons, the greatest biomass of the phytoplankton community was made up of cyanobacteria at two depths and all times of the day. Cylindrospermopsis raciborskii (Woloszynska) Seenayya et Subba Raju was dominant at all times of the day on both the surface and at the bottom. In the rainy season, the differences in cyanobacterial biomass between the surface and bottom were less significant than in the dry season. The differences in cyanobacterial biomass between surface and bottom were less pronounced than those found in the dry season. We concluded that a) physical variables better explain the alterations of species in the phytoplankton community in an environment dominated by cyanobacteria throughout the year; b) seasonal climatic factors associated to periods of stratification and de-stratification are important for alterations in the community and variations in biomass and, c) the turbidity caused by rainfall favored the emergence and establishment of other cyanobacteria, especially Planktothrix agardhii (Gomont) Anagnostidis & Komarek.

Zoocoenological state of microhabitats and its seasonal dynamics in an aquatic macroinvertebrate assembly (Hydrobiological case studies on lake Balaton, No. 1.)

In the years 2002, 2003 and 2004 we collected samples of macroinvertebrates on a total of 36 occasions in Badacsony bay, in areas of open water (in the years 2003 and 2004 reed-grassy) as well as populated by reed (Phragmites australis) and cattail (Typha angustifolia). Samples were taken using a stiff hand net. The sampling site includes three microhabitats differentiated only by the aquatic plants inhabiting these areas. Our data was gathered from processing 208 individual samples. The quantity of macroinvertebrates is represented by biovolume value based on volume estimates. We can identify taxa in abundant numbers found in all water types and ooze; as well as groups associated with individual microhabitats with various aquatic plants. We can observe a notable difference between the years in the volume of invertebrate macrofauna caused by the drop of water level, and the multiplication of submerged macrophytes. There are smaller differences between the samples taken in reeds and cattail stands. In the second half of 2003 – which was a year of drought – the Najas marina appeared in open waters and allowed to support larger quantities of macroinvertebrates. In 2004 with higher water levels, the Potamogeton perfoliatus occurring in the same area has had an even more significant effect. This type of reed-grass may support the most macroinvertebrates during the summer. From the aspect of diversity relations we may suspect different characteristics. The reeds sampling site proved to be the richest, while the cattail microhabitat is close behind, open water (with submerged macrophytes) is the least diverse microhabitat.

Seasonal dynamics of an aquatic macroinvertebrate assembly (Hydrobiological case study of Lake Balaton No. 2)

In 2002, 2003 and 2004, we took macoinvertebrate samples on a total of 36 occasions at the Badacsony bay of Lake Balaton. Our sampling site was characterised by areas of open water (in 2003 and 2004 full of reed-grass) as well as by areas covered by common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia). Samples were taken both from water body and benthic ooze by use of a stiff hand net. We have gained our data from processing 208 individual samples. We took samples frequently from early spring until late autumn for a deeper understanding of the processes of seasonal dynamics. The main seasonal patterns and temporal changes of diversity were described. We constructed a weather-dependent simulation model of the processes of seasonal dynamics in the interest of a possible further utilization of our data in climate change research. We described the total number of individuals, biovolume and diversity of all macroinvertebrate species with a single index and used the temporal trends of this index for simulation modelling. Our discrete deterministic model includes only the impact of temperature, other interactions might only appear concealed. Running the model for different climate change scenarios it became possible to estimate conditions for the 2070-2100 period. The results, however, should be treated very prudently not only because our model is very simple but also because the scenarios are the results of different models.

Analysis of climate change scenarios based on modelling of the seasonal dynamics of a Danubian copepod species

Climate change is one of the most crucial ecological problems of our age with great influence. Seasonal dynamics of aquatic communities are — among others — regulated by the climate, especially by temperature. In this case study we attempted the simulation modelling of the seasonal dynamics of a copepod species, Cyclops vicinus, which ranks among the zooplankton community, based on a quantitative database containing ten years of data from the Danube’s Göd area. We set up a simulation model predicting the abundance of Cyclops vicinus by considering only temperature as it affects the abundance of population. The model was adapted to eight years of daily temperature data observed between 1981 and 1994 and was tested successfully with the additional data of two further years. The model was run with the data series of climate change scenarios specified for the period around 2070- 2100. On the other hand we looked for the geographically analogous areas with the Göd region which are mostly similar to the future climate of the Göd area. By means of the above-mentioned points we can get a view how the climate of the region will change by the end of the 21st century, and the way the seasonal dynamics of a chosen planktonic crustacean species may follow this change. According to our results the area of Göd will be similar to the northern region of Greece. The maximum abundance of the examined species occurs a month to one and a half months earlier, moreover larger variances are expected between years in respect of the abundance. The deviations are expected in the direction of smaller or significantly larger abundance not observed earlier.