Plot size matters: interference from intergenotypic competition in plant phenotyping studies

Genetic and physiological studies often comprise genotypes diverse in vigour, size and flowering time. This can make the phenotyping of complex traits challenging, particularly those associated with canopy development, biomass and yield, as the environment of one genotype can be influenced by a neighbouring genotype. Limited seed and space may encourage field assessment in single, spaced rows or in small, unbordered plots, whereas the convenience of a controlled environment or greenhouse makes pot studies tempting. However, the relevance of such growing conditions to commercial field-grown crops is unclear and often doubtful. Competition for water, light and nutrients necessary for canopy growth will be variable where immediate neighbours are genetically different, particularly under stress conditions, where competition for resources and influence on productivity is greatest. Small hills and rod-rows maximise the potential for intergenotypic competition that is not relevant to a crop’s performance in monocultures. Response to resource availability will typically vary among diverse genotypes to alter genotype ranking and reduce heritability for all growth-related traits, with the possible exception of harvest index. Validation of pot experiments to performance in canopies in the field is essential, whereas the planting of multirow plots and the simple exclusion of plot borders at harvest will increase experimental precision and confidence in genotype performance in target environments.

The effect of potato variety mixtures on epidemics of late blight, in relation to plot size and level of resistance

Potatoes of a number of varieties of contrasting levels of resistance were planted in pure or mixed stands in four experiments over 3 years. Three experiments compared the late blight severity and progress in mixtures with that in pure stands. Disease on susceptible or moderately resistant varieties typical of those in commercial use was similar in mixtures and pure stands. In 2 of 3 years, there were slight reductions on cv. Sante, which is moderately susceptible, in mixture with cv. Cara, which is moderately resistant. Cara was unaffected by this mixture. Mixtures of an immune or near-immune partner with Cara or Sante substantially reduced disease on the latter. The effect of the size of plots of individual varieties or mixtures on blight severity was compared in two experiments. Larger plots had a greater area under the disease progress curve, but the average rate of disease progress was greater in smaller plots; this may be because most disease progress took place later, under more favourable conditions, in the smaller plots. In one experiment, two planting densities were used. Density had no effect on disease and did not interact with mixture effects. The overall conclusion is that, while mixtures of potato varieties may be desirable for other reasons, they do not offer any improvement on the average of the disease resistance of the components.

Determinants of climate change adaptation strategies used by rice farmers in Southwestern, Nigeria

Poor adaptation to climate change is a major threat to sustainable rice production in Nigeria. Determinants of appropriate climate-change adaptation strategies used by rice farmers in Southwestern Nigeria have not been fully investigated. In this study, the determinants of climate change adaptation strategies used by rice farmers in Southwestern Nigeria were investigated. Data were obtained through Focus Group Discussions (FGDs) and field survey conducted in the study areas. Data obtained were analyzed using descriptive and inferential statistical tools such as percentage and regression analysis. The major climate change adaptation strategies used by the respondents included; planting improved rice variety such as Federal Agricultural Research Oryza (FARO) (80.5 %), seeking early warning information (80.9 %), shifting planting date until the weather condition was favourable (99.1 %), and using chemical fertilizer on their farms in order to maintain soil fertility (20.5 %). The determinants of climate change adaptation strategies used by the farmers, included access to early warning information (β=43.04), access to fertilizer (β=5.78), farm plot size (β=–12.04) and access to regular water supply (β=–24.79). Climate change adaptation required provision of incentives to farmers, training on drought and flood control, and the use of improved technology to obtain higher yield.

Development of mechanical methods for cell-tray propagation and field transplanting of dwarf napiergrass (Pennisetum purpureum Schumach.)

Since dwarf napiergrass (Pennisetum purpureum Schumach.) must be propagated vegetatively due to lack of viable seeds, root splitting and stem cuttings are generally used to obtain true-to-type plant populations. These ordinary methods are laborious and costly, and are the greatest barriers for expanding the cultivation area of this crop. The objectives of this research were to develop nursery production of dwarf napiergrass in cell trays and to compare the efficiency of mechanical versus manual methods for cell-tray propagation and field transplanting. After defoliation of herbage either by a sickle (manually) or hand-mowing machine, every potential aerial tiller bud was cut to a single one for transplanting into cell trays as stem cuttings and placed in a glasshouse over winter. The following June, nursery plants were trimmed to a 25–cm length and transplanted in an experimental field (sandy soil) with 20,000 plants ha^(−1) either by shovel (manually) or Welsh onion planter. Labour time was recorded for each process. The manual defoliation of plants required 44% more labour time for preparing the stem cuttings (0.73 person-min. stemcutting^(−1)) compared to using hand-mowing machinery (0.51 person-min. stem-cutting^(−1)). In contrast, labour time for transplanting required an extra 0.30 person-min. m^(−2) (14%) using the machinery compared to manual transplanting, possibly due to the limited plot size for machinery operation. The transplanting method had no significant effect on plant establishment or plant growth, except for herbage yield 110 days after planting. Defoliation of herbage by machinery, production using a cell-tray nursery and mechanical transplanting reduced the labour intensity of dwarf napiergrass propagation.

Persistência do plano experimental em ensaios de avaliação de germoplasma elite de feijão

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

EFEITO da APLICAÇÃO DE UNICONAZOLE NA CULTURADE SOJA [(Glycine max (L.) MERRILL CV IAC-17)]

O presente trabalho foi conduzido no campo com o objetivo de estudar os efeitos da aplicação foliar de uniconazole (S-3307D) no início da floração em plantas de soja (Glycine max (L.) Merrill cv IAC-17). As dosagens de uniconazole utilizadas foram: 2,5; 5,0; 10,0 e 20,0 g i.a./ha. O delineamento experimental utilizado foi de blocos ao acaso, com 5 tratamentos contendo 4 repetições cada, sendo o tamanho de cada parcela de 40 m2, totalizando 800 m2 de área experimental. Para a avaliação dos efeitos do S-3307D sobre a produtividade de plantas de soja, foram realizadas as seguintes observações, no momento da colheita: produção (peso em Kg das plantas em 10 m2); número de plantas/10 m2; número de vagens/planta; número de grãos/vagem; peso de 100 grãos (em g); altura da planta (em cm) e número de ramos. A partir dos resultados obtidos pode-se concluir que uniconazole a 10,0 g i.a./ha, foi eficiente em aumentar a produtividade da cultura de soja (Glycine max (L.) Merrill cv IAC-17).

Effect of tillage on fractal indices describing soil surface microrelief of a Brazilian Alfisol

Soil surface roughness is known to influence water infiltration, runoff and erosion. Soil surface roughness changes with management and weather and its mathematical description still remains an important issue. The main objective of this study was to investigate the effect of tillage on the two fractal indices, fractal dimension, D, and crossover length, 1, currently used in characterizing soil surface microrelief. The statistical index random roughness, RR, was also assessed. Field experiments were done on an Alfisol located at Rio Grande do Sul State (Brazil). Two tillage treatments (conventional versus direct drilling) were tested. The soil surface microrelief was assessed by point elevation measurements in 16 plots for each treatment. The sampling scheme was a square grid with 20 x 20 mm between point spacing and the plot size was 280 x 280 mm, so that each data set consisted of 225 individual elevation points. All indices were calculated after trend removal, both by slope correction, i.e., oriented microrelief, and by slope plus tillage marks correction, i.e., random microrelief. The implemented algorithm for estimating D and 1 consisted in evaluating the roughness around the local root mean square deviation (RMS) of the point elevation values. Irrespective of tillage treatment and detrending procedure, fractal behavior extended only over a bounded range of scales, from 40 to 100 mm, due to the experimental setup. In these conditions, assessing fractal indices was not always straightforward. The statistical index RR and the fractal index I were significantly different between tillage treatments for oriented and random surface conditions. D values of random soil surfaces were not affected by tillage treatment, whereas D values of oriented microrelief were significantly lower in the direct drilled plots. Removal of tillage marks trend resulted in a significant increase in D values. Within each tillage treatment, 1 and D were significantly correlated. (c) 2006 Elsevier B.V. All rights reserved.

Uso de diferentes telas de sombreamento no cultivo protegido do tomateiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

An Investigation of Wild Bee Diversity and Abundance in Plots Managed by The Nature Conservancy in South-Central Nebraska and of Beneficial Arthropods Associated with Native Nebraska Flora

Insect pollination is an essential ecosystem service, and bees are the principal pollinators of wild and cultivated plants. Habitat management and enhancement are a proven way to encourage wild bee populations, providing them with food and nesting resources. I examined bee diversity and abundance in plots managed by The Nature Conservancy near Wood River, NE. The plots were seeded with 2 seed mixes at 2 seeding rates: high diversity mix at the recommended rate, high diversity mix double the recommended rate, Natural Resources Conservation Service (NRCS) conservation planting (CP) 25 mix at one-half the recommended rate, and NRCS CP25 mix at the recommended rate. I measured wild bee abundance and diversity, and established a database of wild bees associated with the plots. I also compared genus richness and abundance among the plots using and aerial net and blue vane traps to collect bees. Significant differences were not observed in genus richness and diversity among the plots; however, plot size and the ability of blue vane traps to draw bees from a long distance may have influenced my results. In 2008, 15 genera and 95 individual bees were collected using an aerial net and in 2009, 32 genera and 6,103 individual bees were collected using blue vane traps. I also studied the beneficial insects associated with native Nebraska flora. Seventeen species of native, perennial flora were established in 3 separate plots located in eastern Nebraska. I transplanted four plants of each species in randomized 0.61 m x 0.61 m squares of a 3.05 m x 9.14 m plot. Arthropods were sampled using a modified leaf blower/vacuum. Insects and other arthropods were identified to family and organized into groups of predators, parasites, pollinators, herbivores, and miscellaneous. Associations between plant species and families of beneficial arthropods (predators, parasites, and pollinators) were made. Pycnanthemum flexuosum Walter attracted significantly more beneficial arthropod families than 7 other species of plants tested. Dalea purpurea Vent and Liatris punctata Hook also attracted significantly fewer beneficial arthropod families than 4 other species of plants tested. In total, 31 predator, 11 parasitic, 4 pollinator, 31 herbivore, and 10 miscellaneous families of arthropods were recorded.

Mangrove Forests and Sedimentary Processes on the South of Coast of Sao Paulo State (Brazil)

Mangrove structure and distribution is conditioned by geomorphic processes. This paper describes the response of mangroves to sedimentary processes at the Cananeia-Iguape Coastal System on the south coast of Sao Paulo State (Brazil), between latitudes 24 degrees 40`S and 25 degrees 20`S. Within six study areas 41 plots were established along 14 transects. Plot size varied according to stem density from 2mx2m to 20mx20m. Here mangroves are strongly coupled to sedimentary processes, forming discrete architectural elements within particular depositional environments or topographic settings. These sedimentary structures and progradation environments are colonized by Laguncularia racemosa, associated with the smooth cordgrass Spartina alterniflora. Rhizophora mangle occurs typically near creeklets where tidal flooding is more frequent. Where tidal influence is restricted Avicennia schaueriana becomes dominant. Erosive margins are dominated by A. schaueriana or R. mangle. Single linkage cluster analysis yields three groups (A, B and C), with high levels of similarity, providing support to the classification of the data into two broad landform categories: depositional and erosive. Group A includes plots with the least structural development (nominal stem diameter d(n) between 1.05 and 4.61cm). Group B is composed of stems of intermediate diameter (4.99 cm <= d(n) <= 5.63cm). Group C plots have the largest structural development (5.50 cm <= d(n) <= 11.10cm). The structure of mangroves (dominance and structural development) reflects responses to geomorphology and habitat change.

A comparison of ground cover and frequency estimation methods for post-harvest soil monitoring

The amount and type of ground cover is an important characteristic to measure when collecting soil disturbance monitoring data after a timber harvest. Estimates of ground cover and bare soil can be used for tracking changes in invasive species, plant growth and regeneration, woody debris loadings, and the risk of surface water runoff and soil erosion. A new method of assessing ground cover and soil disturbance was recently published by the U.S. Forest Service, the Forest Soil Disturbance Monitoring Protocol (FSDMP). This protocol uses the frequency of cover types in small circular (15cm) plots to compare ground surface in pre- and post-harvest condition. While both frequency and percent cover are common methods of describing vegetation, frequency has rarely been used to measure ground surface cover. In this study, three methods for assessing ground cover percent (step-point, 15cm dia. circular and 1x5m visual plot estimates) were compared to the FSDMP frequency method. Results show that the FSDMP method provides significantly higher estimates of ground surface condition for most soil cover types, except coarse wood. The three cover methods had similar estimates for most cover values. The FSDMP method also produced the highest value when bare soil estimates were used to model erosion risk. In a person-hour analysis, estimating ground cover percent in 15cm dia. plots required the least sampling time, and provided standard errors similar to the other cover estimates even at low sampling intensities (n=18). If ground cover estimates are desired in soil monitoring, then a small plot size (15cm dia. circle), or a step-point method can provide a more accurate estimate in less time than the current FSDMP method.

Césped deportivo : comportamiento del cultivar AZ-1 de Pennisetum clandestinum Hochst. ex Chiov. (kikuyo). Paraná, Entre Ríos, Argentina

A fines de 2001 se incorporó al mercado argentino el cultivar AZ-1 de Pennisetum clandestinum Hochst. ex Chiov. (kikuyo) para ser utilizado en campos deportivos o con fines ornamentales. Con el propósito de caracterizar esta variedad comercial en el departamento Paraná (provincia de Entre Ríos, Argentina) se evaluó su comportamiento y aptitud para césped ornamental y/o deportivo en condiciones de mantenimiento para césped de calidad, bajo dos condiciones de drenaje. Se evaluaron cobertura, color, textura, distancia de entrenudos, grosor de estolones y período de dormición. El ensayo se realizó en Oro Verde, departamento Paraná, en un suelo Molisol y consistió en 2 tratamientos: con y sin drenaje, con 4 repeticiones cada uno. El tamaño de la parcela fue de 2,5 por 5,0 m. El diseño experimental utilizado fue parcelas apareadas y las mediciones se realizaron desde junio hasta noviembre de 2005. Las características climáticas locales son: temperatura media anual de 18,1°C y un régimen isohigro de 947,6 mm de precipitación anual. El cultivar se comportó como apto para césped en las características y condiciones de manejo evaluadas, sin diferencias entre los tratamientos con y sin drenaje. Presentó rápida implantación, niveles altos de cobertura, color claro y uniforme, textura media y estolones gruesos a medianos a través del tiempo. En invierno no perdió cobertura ni color. Se caracterizó como un césped no apto para campos deportivos de alta exigencia, pero recomendable para clubes de bajo presupuesto y mantenimiento.

Eficiencia de control de "orugas defoliadoras" en soja (Glycine max L.), con insecticidas neurotóxicos y reguladores del crecimiento de los insectos

Las "orugas defoliadoras" afectan la producción del cultivo de soja, sobre todo en años secos y con altas temperaturas que favorecen su desarrollo. El objetivo del presente trabajo fue evaluar la eficiencia de control de insecticidas neurotóxicos e IGRs sobre "orugas defoliadoras" en soja. Se realizaron ensayos en lotes comerciales en tres localidades de la provincia de Córdoba en las campañas agrícolas 2008/09 y 2009/10, bajo un diseño de bloques al azar, con seis tratamientos y tres repeticiones. Los tratamientos fueron: T1: Clorpirifos (384 g p.a.ha-1), T2: Cipermetrina (37,5 g p.a.ha-1), T3: Lufenuron+Profenofos (15 + 150 g p.a.ha-1), T4: Metoxifenocide (28,8 g p.a.ha-1), T5: Novaluron (10 g p.a.ha-1) y T6: Testigo. El tamaño de las parcelas fue de 12 surcos de 10 m de largo distanciados a 0,52 m. La aplicación se realizó con una mochila provista de boquillas de cono hueco (40 gotas.cm-2), cuando la plaga alcanzó el umbral de daño económico. En cada parcela se tomaron cinco muestras a los 0, 2, 7 y 14 días después de la aplicación (DDA) utilizando el paño vertical, identificando y cuantificando las orugas vivas mayores a 1,5 cm. A los 14 DDA se extrajeron 30 folíolos por parcela (estrato medio y superior de la planta) y se determinó el porcentaje de defoliación utilizando el software WinFolia Reg. 2004. Se estimó el rendimiento sobre 5 muestras de 1 m2 en cada parcela y se realizó ANOVA y test de comparación de medias LSD de Fisher. El Clorpirifos mostró el mayor poder de volteo y el Metoxifenocide la mayor eficiencia a los 7 DDA. En general los IGRs mostraron mayor poder residual.

Productores agropecuarios, pampa ondulada y problemáticas edáficas. Un estudio de caso en el noreste bonaerense

El carácter de la heterogeneidad social, económica y productiva de la agriculturización se evidencia en los diversos tipos de productores existentes en la pampa húmeda. La agriculturización se asocia a modificaciones en la calidad de las tierras, en la estructura socio-productiva, en las estrategias productivas aplicadas y en las formas de uso del suelo. Suele plantearse sin embargo que las problemáticas edáficas asociadas a dicha agriculturización, son consecuencia de la aplicación de paquetes tecnológicos relativamente homogéneos, independientemente de los diferentes tipos de productores que los llevan a cabo. Nuestra hipótesis consiste en cambio, en que el deterioro en Argiudoles típicos pampeanos es el resultado, dadas distintas posiciones del relieve, de complejas combinaciones de diversas estrategias productivas adoptadas por diferentes tipos de productores. En el partido de Luján tipificamos los productores tomando en consideración sus niveles de capitalización (capitalizados y no capitalizados) y su organización del trabajo (familiar y no familiar). Definimos cinco estrategias productivas (4 agrícolas -con uno o dos cultivos por año, con siembra directa o labranza convencional- y 1 ganadera) y dos ambientes (loma y bajo). A partir del catastro municipal aplicamos una encuesta a una muestra estratificada estadísticamente representativa, por ubicación y tamaño de lotes. Sobre la misma realizamos el muestreo que nos permitió analizar los siguientes parámetros: profundidad del horizonte, densidad aparente, materia orgánica, acidez, nitrógeno, fósforo, potasio. Calculamos el contenido de materia orgánica y de nitrógeno por hectárea, y el deterioro relativo. Realizamos test de hipótesis de comparación de medias, prueba F y prueba t; y, calculamos finalmente los deterioros relativos. Utilizamos como indicador el contenido de materia orgánica por hectárea, dada su mayor sensibilidad a los cambios en las condiciones del suelo. Dos de nuestros principales hallazgos nos indican, en primer lugar, que al cultivar en lomas y con siembra directa, todos los tipos de productores presentaron los valores más bajos de deterioro relativo, excepto los familiares no capitalizados que obtienen los menores deterioros relativos con labranza convencional, aún en los bajos. Estos productores utilizan la ganadería en rotación con los cultivos como táctica de cuidado del suelo. Los empresarios capitalizados presentan los mayores valores de deterioro relativo en los bajos. Los familiares presentan menores pérdidas, cuando son capitalizados logran las mejores situaciones relativas al aplicar siembra directa en los bajos. En segundo lugar, ninguna dimensión -tipo de productor, estrategias productivas, ambientes- analizada aisladamente determina el deterioro relativo de los suelos. Pero, al mismo tiempo, ninguno puede ser descartado sino que debería ser incluido en una combinación que integre las condiciones de ambiente y las estrategias productivas de los diferentes tipos de productores que manejan los suelos.

Productores agropecuarios, pampa ondulada y problemáticas edáficas. Un estudio de caso en el noreste bonaerense

El carácter de la heterogeneidad social, económica y productiva de la agriculturización se evidencia en los diversos tipos de productores existentes en la pampa húmeda. La agriculturización se asocia a modificaciones en la calidad de las tierras, en la estructura socio-productiva, en las estrategias productivas aplicadas y en las formas de uso del suelo. Suele plantearse sin embargo que las problemáticas edáficas asociadas a dicha agriculturización, son consecuencia de la aplicación de paquetes tecnológicos relativamente homogéneos, independientemente de los diferentes tipos de productores que los llevan a cabo. Nuestra hipótesis consiste en cambio, en que el deterioro en Argiudoles típicos pampeanos es el resultado, dadas distintas posiciones del relieve, de complejas combinaciones de diversas estrategias productivas adoptadas por diferentes tipos de productores. En el partido de Luján tipificamos los productores tomando en consideración sus niveles de capitalización (capitalizados y no capitalizados) y su organización del trabajo (familiar y no familiar). Definimos cinco estrategias productivas (4 agrícolas -con uno o dos cultivos por año, con siembra directa o labranza convencional- y 1 ganadera) y dos ambientes (loma y bajo). A partir del catastro municipal aplicamos una encuesta a una muestra estratificada estadísticamente representativa, por ubicación y tamaño de lotes. Sobre la misma realizamos el muestreo que nos permitió analizar los siguientes parámetros: profundidad del horizonte, densidad aparente, materia orgánica, acidez, nitrógeno, fósforo, potasio. Calculamos el contenido de materia orgánica y de nitrógeno por hectárea, y el deterioro relativo. Realizamos test de hipótesis de comparación de medias, prueba F y prueba t; y, calculamos finalmente los deterioros relativos. Utilizamos como indicador el contenido de materia orgánica por hectárea, dada su mayor sensibilidad a los cambios en las condiciones del suelo. Dos de nuestros principales hallazgos nos indican, en primer lugar, que al cultivar en lomas y con siembra directa, todos los tipos de productores presentaron los valores más bajos de deterioro relativo, excepto los familiares no capitalizados que obtienen los menores deterioros relativos con labranza convencional, aún en los bajos. Estos productores utilizan la ganadería en rotación con los cultivos como táctica de cuidado del suelo. Los empresarios capitalizados presentan los mayores valores de deterioro relativo en los bajos. Los familiares presentan menores pérdidas, cuando son capitalizados logran las mejores situaciones relativas al aplicar siembra directa en los bajos. En segundo lugar, ninguna dimensión -tipo de productor, estrategias productivas, ambientes- analizada aisladamente determina el deterioro relativo de los suelos. Pero, al mismo tiempo, ninguno puede ser descartado sino que debería ser incluido en una combinación que integre las condiciones de ambiente y las estrategias productivas de los diferentes tipos de productores que manejan los suelos.