Critically endangered Pristis microdon (Elasmobranchii), as a host for the Indian parasitic copepod, Caligus furcisetifer Redkar, Rangnekar et Murti, 1949 (Siphonostomatoida): New records from northern Australia.

This paper presents the first records of the parasitic copepod Caligus furcisetifer Redkar, Rangnekar et Murti, 1949 beyond Indian waters, specifically, on the body surface and head of the critically endangered largetooth sawfish (commonly referred to as the freshwater sawfish in Australia), Pristis microdon Latham, 1794 (Elasmobranchii, Pristidae), in brackish tidal waters of the Fitzroy River in the Kimberley region of Western Australia and the Leichhardt River in the Gulf of Carpentaria in northern Queensland. This represents a geographic range extension of similar to 8000 km for this parasite. Further, it is only the second member of the genus Caligus to be found on an elasmobranch host in Western Australia and it is the first time this species has been reported from the Southern Hemisphere. Male biased dispersal of P microdon may be the vector in which the parasite has dispersed from India across to northern Australia, or vice versa. A decline in populations of the critically endangered P microdon (and possibly other pristid species) in these regions may lead to a concomitant decline in their parasite fauna.

Identification of immune genes in grass carp Ctenopharyngodon idella in response to infection of the parasitic copepod Sinergasilus major

The parasitic copepod Sinergasilus major is an important pathogen of grass carp Ctenopharyngodon idella. To understand the immune response of grass carp to the copepod infection, suppression subtractive hybridization method was employed to characterize genes up-regulation during the copepod infection in liver and gills of the fish. One hundred and twenty-two dot blot positive clones from infected subtracted library were sequenced. Searching available databases by using these nucleotide sequences revealed that 23 genes are immune-related, including known acute-phase reactants, and four novel genes encoding proteins such as source of immunodominant MHC-associated peptides (SIMP), TNF receptor-associated factor 2 binding protein (T2BP), poliovirus receptor-related protein 1 precursor, glycoprotein A repetitions predominant (GARP). The differential expression of seven immune genes, i.e. GARP, alpha-2-macroglobulin, MHC class I, C3, SIMP, T2BP, transferrin, as a result of infection was further confirmed by RT-PCR, with the up-regulation of alpha-2-macroglobulin, MHC class I, C3, SIMP and T2BP in the liver of infected fish, and down-regulation of SIMP in the gills of infected fish. The present study provides foundation for understanding grass carp immune response and candidate genes for further analysis.

Mortalities induced by the copepod Sinergasilus polycolpus in farmed silver and bighead carp in a reservoir

The frequency distributions of the parasitic copepod Sinergasilus polycolpus were examined in silver carp Hypophthalmichthys molitrix and bighead carp Aristichthys nobilis during a disease outbreak of the 2 species of fish in a reservoir in China. The mean abundance of the copepod was positively related with host length and age, and the overdispersion of the copepod in both silver and bighead carp was fitted well with negative binomial distribution. Although parasite-induced host mortality was observed, a peaked age-parasite abundance curve was not detected in the present parasite-host system. It is also proposed that this peaked age-abundance curve is unlikely to be observed in its natural host populations.

Population Biology and Life History of the North American Menhadens, Brevoortia spp.

Four recognized species of menhaden, Brevoortia spp., occur in North American marine waters: Atlantic menhaden, B. tyrannus; Gulf menhaden, B. patronus; yellowfin menhaden. B. smithi; and finescale menhaden, B. gunteri. Three of the menhaden species are known to form two hybrid types. Members of the genus range from coastal waters of Veracruz, Mex., to Nova Scotia, Can. Atlantic and Gulf menhaden are extremely abundant within their respective ranges and support extensive purse-seine reduction (to fish meal and oil) fisheries. All menhaden species are estuarine dependent through late larval and juvenile stages. Depending on species and location within the range, spawning may occur within bays and sounds to a substantial distance offshore. Menhaden are considered to be filter-feeding, planktivorous omnivores as juveniles and adults. Menhaden eggs, immature developmental stages, and adults are potential prey for a large and diverse number of predators. North American menhadens, including two hybrids, are hosts for the parasitic isopod, Olencira praegustator, and the parasitic copepod, Lemaeenicus radiatus. Although the data are quite variable, a dome-shaped Ricker function is frequently used to describe the spawner-recruitment relationship for Atlantic and Gulf menhaden. Each of these species is treated as a single stock with respect to exploitation by the purse-seine reduction fishery. Estimates of instantaneous natural (other) mortality rates are O.45 for Atlantic menhaden and 1.1 for Gulf menhaden.

Effects of land use and physicochemical water quality on grass shrimp, Palaemonetes pugio, and its parasitic isopod, Probopyrus pandalicola, in South Carolina, USA tidal creeks

Grass shrimp, Palaemonetes pugio, are a common inhabitant of US East and Gulf coast salt marshes and are a food source for recreationally and economically important fish and crustacean species. Due to the relationship of grass shrimp with their ecosystem, any significant changes in grass shrimp population may have the potential to affect the estuarine system. Land use is a crucial concern in coastal areas where increasing development impacts the surrounding estuaries and salt marshes and has made grass shrimp population studies a logical choice to investigate urbanization effects. Any impact on tidal creeks will be an impact on grass shrimp populations and their associated micro-environment whether predator, prey or parasitic symbiont. Anthropogenic stressors introduced into the grass shrimp ecosystem may even change the intensity of infections from parasitic symbionts. An ectoparasite found on P. pugio is the bopyrid isopod Probopyrus pandalicola. Little is known about factors that may affect the occurrence of this isopod in grass shrimp populations. The goal was to analyze the prevalence of P. pandalicola in grass shrimp in relation to land use classifications, water quality parameters, and grass shrimp population metrics. Eight tidal creeks in coastal South Carolina were sampled monthly over a three year period. The occurrence of P. pandalicola ranged from 1.2% to 5.7%. Analysis indicated that greater percent water and marsh coverage resulted in a higher incidence of bopyrid occurrence. Analysis also indicated that higher bopyrid incidence occurred in creeks with higher salinity, temperature, and pH but lower dissolved oxygen. The land use characteristics found to limit bopyrid incidence were limiting to grass shrimp (definitive host) populations and probably copepod (intermediate host) populations as well.

Some parasitic Copepoda from Sri Lanka (Ceylon) with a synopsis of parasitic Crustacea from Ceylonese freshwater fishes

This report comprises a summary of parasitic copepods from fishes in Ceylon, as isolated from the branchial material of fishes belonging to previous collections. Seven copepod species are described in detail, as well as one species of Branchiura and one species of Isopoda. Caution is advocated to avoid further introductions via parasite-infested fishes, since only four of the above species are endemic.

Studies on Copepod Parasites on Elasmobranchs of Kerala Coast

The present study of the parasitic copepods gives the taxonomic description of thirty one species of parasites collected from various elasmobranch fishes of Kerala coast. Copepods parasitic on fishes include three sub orders, viz. poecilostomatoida, cyclopoida and siphonostomatoida. Parasitic copepods of elasmobranch fishes for the present study were collected from different fish landing centres of Kerala and by undertaking regular fishing trips. The collected parasites are identified to the species level and described. It is found that out of thirty one species, fifteen are new to science. They belong to the genera viz. Nothobomolochus Vervoot, 1962, Caligus Muller, 1785, Alebion, Kroyer, 1863, Gloipotes Steenstrup and Lutken, 1861, Pandarus Leach,1819, Perissopus Steenstrup and Lutken, 1861, Echthrogaleus Steenstrup and Lutken, 1861 and Kroyeria van Beneden, 1853. Fifteen new host records were reported. Two genera viz. Echthrogaleus and Entepherus were reported for first time from Indian waters. A new genus called Penicillus was erected. The general observations made during this study revealed certain interesting aspects of host-parasite relationship, host specificity, site specificity, adaptive modifications and geographical distribution.

Studies on The Copepod Fish Parasites of Kerala Coast

The present study of the parasitic copepods gives an account of the taxonomic description of seventy seven species of parasites collected from the food fishes of the Kerala coast. Out of the seventy seven species described, fourteen are new to science, two new records for the Indian waters and ten new host records. The males of Parapetalus longipinnatus Rangnekar and Lerna~thropus indicus Pillai were collected and described for the first time. The parasites described belong to the suborders Cyclopoida, Caligoida and Lernaeopodoida. The available description of many species of this locality is reviewed and supplemented with the help of the present detailed study. The general observations made during this study reveale certain interesting aspects of the host parasite relationship, host specificity, adaptive modification and geographical distribution. A brief discussion of these observations made is also presented.

Studies on the freshwater copepod fish parasites of Kerala

Studies on parasitic copepods from freshwater fishes are still in its infancy. In recent years, there- is a renewed enthusiasm in the study of freshwater fish parasites due to rapidly increasing aquaculture practices. The importance of diseases and their control assumes great significance because of the adverse impact of diseases on fish production and its economy. Copepods are one of the most harmful parasites of freshwater fishes. Reports on the damages caused by copepod parasites from different parts on the world are increasing alarmingly. But the information on parasitic copepods of freshwater fishes in India is quite meagre. Knowledge regarding this group of parasites, their Biology and pathology from Kerala. is lacking. The thesis consists of five chapters. The first chapter is a general introduction which deals with the review of literature on various aspects of parasitic copepods viz; systematics, life history, host-parasite relationship, ecology, pathogenicity, prophylaxis and control measures. Systematics of parasitic copepods of freshwater fishes collected during the present study forms the second chapter. The third chapter deals with the life cycle study of the new Lernaeid copepod, Lernaea osphronemi. The fourth chapter contains host-parasite relationship. ecology and treatment of ‘the’ new species of Lernaea On Osphronemus goramy. General observations and a summary of the entire work constitute the fifth chapter

Host-delivered RNAi: An effective strategy to silence genes in plant parasitic nematodes

Root-knot nematodes (Meloidogyne spp.) are obligate, sedentary endoparasites that infect many plant species causing large economic losses worldwide. Available nematicides are being banned due to their toxicity or ozone-depleting properties and alternative control strategies are urgently required. We have produced transgenic tobacco (Nicotiana tabacum) plants expressing different dsRNA hairpin structures targeting a root-knot nematode (Meloidogyne javanica) putative transcription factor, MjTis11. We provide evidence that MjTis11 was consistently silenced in nematodes feeding on the roots of transgenic plants. The observed silencing was specific for MjTis11, with other sequence-unrelated genes being unaffected in the nematodes. Those transgenic plants able to induce silencing of MjTis11, also showed the presence of small interfering RNAs. Even though down-regulation of MjTis11 did not result in a lethal phenotype, this study demonstrates the feasibility of silencing root-knot nematode genes by expressing dsRNA in the host plant. Host-delivered RNA interference-triggered (HD-RNAi) silencing of parasite genes provides a novel disease resistance strategy with wide biotechnological applications. The potential of HD-RNAi is not restricted to parasitic nematodes but could be adapted to control other plant-feeding pests.