The Fate of Chrysotile-Induced Multipolar Mitosis and Aneuploid Population in Cultured Lung Cancer Cells

Chrysotile is one of the six types of asbestos, and it is the only one that can still be commercialized in many countries. Exposure to other types of asbestos has been associated with serious diseases, such as lung carcinomas and pleural mesotheliomas. The association of chrysotile exposure with disease is controversial. However, in vitro studies show the mutagenic potential of chrysotile, which can induce DNA and cell damage. The present work aimed to analyze alterations in lung small cell carcinoma cultures after 48 h of chrysotile exposure, followed by 2, 4 and 8 days of recovery in fiber-free culture medium. Some alterations, such as aneuploid cell formation, increased number of cells in G2/M phase and cells in multipolar mitosis were observed even after 8 days of recovery. The presence of chrysotile fibers in the cell cultures was detected and cell morphology was observed by laser scanning confocal microscopy. After 4 and 8 days of recovery, only a few chrysotile fragments were present in some cells, and the cellular morphology was similar to that of control cells. Cells transfected with the GFP-tagged alpha-tubulin plasmid were treated with chrysotile for 24 or 48 h and cells in multipolar mitosis were observed by time-lapse microscopy. Fates of these cells were established: retention in metaphase, cell death, progression through M phase generating more than two daughter cells or cell fusion during telophase or cytokinesis. Some of them were related to the formation of aneuploid cells and cells with abnormal number of centrosomes.

A Rússia na ordem mundial: com o Ocidente, com o Oriente ou um pólo autônomo em um mundo multipolar?

O artigo persegue o objetivo de definir o lugar e o papel da Rússia nas relações internacionais contemporâneas nos últimos anos. Ao se debruçar sobre o dilema tradicional da política externa russa - Ocidentalismo versus Orientalismo - o autor analisa o cenário de multipolaridade defendido pela nova concepção da política externa russa e o relaciona com a fase do pragmatismo e do multilateralismo que caracteriza a atuação internacional da Rússia de Putin, fazendo considerações, decorrentes do impacto dos ataques terroristas aos Estados Unidos em 11 de setembro de 2001 sobre a política externa russa. A atitude pragmática e a natureza multivetorial da política externa russa contribuem, segundo o autor, para o fortalecimento das posições internacionais da Rússia em comparação com a perda ou a natureza incerta das alianças e dos relacionamentos do período da transição pós-soviética.

Treatment of atrial fibrillation with radiofrequency ablation and simultaneous multipolar mapping of the pulmonary veins

OBJECTIVE: To demonstrate the feasibility and safety of simultaneous catheterization and mapping of the 4 pulmonary veins for ablation of atrial fibrillation. METHODS: Ten patients, 8 with paroxysmal atrial fibrillation and 2 with persistent atrial fibrillation, refractory to at least 2 antiarrhythmic drugs and without structural cardiopathy, were consecutively studied. Through the transseptal insertion of 2 long sheaths, 4 pulmonary veins were simultaneously catheterized with octapolar microcatheters. After identification of arrhythmogenic foci radiofrequency was applied under angiographic or ultrasonographic control. RESULTS: During 17 procedures, 40 pulmonary veins were mapped, 16 of which had local ectopic activity, related or not with the triggering of atrial fibrillation paroxysms. At the end of each procedure, suppression of arrhythmias was obtained in 8 patients, and elimination of pulmonary vein potentials was accomplished in 4. During the clinical follow-up of 9.6±3 months, 7 patients remained in sinus rhythm, 5 of whom were using antiarrhythmic drugs that had previously been ineffective. None of the patients had pulmonary hypertension or evidence of stenosis in the pulmonary veins. CONCLUSION: Selective and simultaneous catheterization of the 4 pulmonary veins with microcatheters for simultaneous recording of their electrical activity is a feasible and safe procedure that may help ablation of atrial fibrillation.

Structural challenges in a multipolar and multicultural global legal era : BRICS’ global legal politics beyond cultural and economic partnerships

New emerging international dynamics introduce a global poly-axiological polycentric disorder which undermines the tradition of a unique global legal order in international law. Modern Era was characterized by Western European civilizational model – from which human rights is a byproduct. This consensus had its legitimacy tested by XXst century’s scenario – and the ‘BRICS factor/actor’ is a symptom of this reality. Its empowerment in world politics lead to the rise of distinct groups of States/civilizations provided with different legal, political, economic and social traditions – promoting an unexpected uprise of otherness in international legal order and inviting it to a complete and unforeseeable reframing process. Beyond Washington or Brussels Consensus, other custom-originated discourses (Brasília, Moscow, New Delhi, Peking or Cape Town Consensus, among other unfolded possibilities) will probably henceforth attempt shaping international law in present global legal disorder.

High precision calculation of multipolar dynamic polarizabilities and two- and three-body dispersion coefficients of atomic hydrogen

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Multipolar polarizabilities of the sodium atom by a variationally stable procedure

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Los BRICS. El surgimiento de un proyecto de nueva arquitectura financiera y de un nuevo mundo multipolar (2009-2014)

En el presente trabajo, se busca indagar en las disputas de proyectos estratégicos a nivel mundial que se profundizan a partir de la crisis financiera global desatada en 2008. En este marco, comienza a plantearse desde distintos sectores económicos, políticos e intelectuales una crítica al sistema mundial heredado de Bretton Woods, al calor del surgimiento de nuevos espacios de poder que plantean la necesidad de avanzar hacia la conformación de un mundo "multipolar", superando la hegemonía norteamericana y europea. En este marco, la aparición en escena de los países denominados "BRICS" (Brasil, Rusia, India, China y Sudáfrica) plantea nuevos interrogantes sobre la coyuntura política actual y sobre la continuidad de la hegemonía trilateral global clásica (Estados Unidos, Europa, Japón). Este trabajo se propondrá analizar el proceso de configuración de los BRICS en el escenario geopolítico general sobre el cual se construye, e indagar en las dimensiones territoriales, políticas, económicas y estratégicas de la integración. Además, buscaremos explorar en los debates que emergen al interior del bloque en torno a las estrategias y modalidades de la integración, intentando dar cuenta de los actores que las promueven, bajo que concepciones, con qué objetivos, que conflictos se suscitan y qué condiciones se observan. Finalmente, nos propondremos indagar algunos alcances económicos, políticos y sociales del proyecto de los BRICS, a partir de las conclusiones emanadas de la VI Cumbre anual del bloque, realizada en Fortaleza, Brasil, entre el 14 y el 16 de julio de 2014

Los BRICS. El surgimiento de un proyecto de nueva arquitectura financiera y de un nuevo mundo multipolar (2009-2014)

En el presente trabajo, se busca indagar en las disputas de proyectos estratégicos a nivel mundial que se profundizan a partir de la crisis financiera global desatada en 2008. En este marco, comienza a plantearse desde distintos sectores económicos, políticos e intelectuales una crítica al sistema mundial heredado de Bretton Woods, al calor del surgimiento de nuevos espacios de poder que plantean la necesidad de avanzar hacia la conformación de un mundo "multipolar", superando la hegemonía norteamericana y europea. En este marco, la aparición en escena de los países denominados "BRICS" (Brasil, Rusia, India, China y Sudáfrica) plantea nuevos interrogantes sobre la coyuntura política actual y sobre la continuidad de la hegemonía trilateral global clásica (Estados Unidos, Europa, Japón). Este trabajo se propondrá analizar el proceso de configuración de los BRICS en el escenario geopolítico general sobre el cual se construye, e indagar en las dimensiones territoriales, políticas, económicas y estratégicas de la integración. Además, buscaremos explorar en los debates que emergen al interior del bloque en torno a las estrategias y modalidades de la integración, intentando dar cuenta de los actores que las promueven, bajo que concepciones, con qué objetivos, que conflictos se suscitan y qué condiciones se observan. Finalmente, nos propondremos indagar algunos alcances económicos, políticos y sociales del proyecto de los BRICS, a partir de las conclusiones emanadas de la VI Cumbre anual del bloque, realizada en Fortaleza, Brasil, entre el 14 y el 16 de julio de 2014