Downregulation of the motA gene delays the escape of the obligate predator Bdellovibrio bacteriovorus 109J from bdelloplasts of bacterial prey cells

Bdellovibrio bacteriovorus is a Gram-negative bacterium that preys on other Gram-negative bacteria. The lifecycle of B. bacteriovorus alternates between an extracellular flagellated and highly motile non-replicative attack-phase cell and a periplasmic non-flagellated growth-phase cell. The prey bacterium containing periplasmic bdellovibrios becomes spherical but osmotically stable, forming a structure known as the bdelloplast. After completing the growth phase, newly formed bdellovibrios regain their flagellum and escape the bdelloplast into the environment, where they encounter more prey bacteria. The obligate predatory nature of B. bacteriovorus imposes a major difficulty to introducing mutations in genes directly involved in predation, since these mutants could be non-viable. This work reports the cloning of the B. bacteriovorus 109J motAB operon, encoding proteins from the flagellar motor complex, and a genetic approach based on the expression of a motA antisense RNA fragment to downregulate motility. Periplasmic bdellovibrios carrying the plasmid expressing antisense RNA displayed a marked delay in escaping from bdelloplasts, while the released attack-phase cells showed altered motility. These observations suggest that a functionally intact flagellar motor is required for the predatory lifecycle of B. bacteriovorus. Also, the use of antisense RNA expression may be a useful genetic tool to study the Bdellovibrio developmental cycle.

Processo de internacionalização das filiais portuguesas de construção civil para Angola: alguns estudos de caso (Mota-Engil, Soares da Costa e Conduril)

Dissertação apresentada no Instituto Superior de Contabilidade e Administração do Instituto Politécnico do Porto para obtenção do Grau de Mestre em Empreendedorismo e Internacionalização Orientador: Professor Doutor José de Freitas Santos

A palavra do corpo em João Mota : um estudo sobre a presença, o corpo e a palavra nos espetáculos "Cyrano de Bergerac" e "Play Strindberg" : dissertação

Dissertação submetida à Escola Superior de Teatro e Cinema para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Artes Performativas - especialização em Interpretação

Subsistema de gestão financeira e contabilística do grupo Mota-Engil Serviços Partilhados (MESP)

Relatório de estágio apresentado ao Instituto Politécnico do Porto para obtenção do Grau de Mestre em Gestão das Organizações, Ramo de Gestão de Empresas

Mota-Engil new prospects of growth: Overcoming the financial crisis

The 2008 global financial crisis caused the collapse of business key sectors, declines in consumer wealth and a fall in economic activity resulting in a global recession. In some European countries, the 2008 crisis contributed to a sovereign-debt crisis which had a strong impact in Southern European countries. The construction sector was particularly affected, with budget cuts disturbing public investment and no financing available for private constructors. This report intends to explain how Mota-Engil, faced this situation of low growth, and which strategies were adopted by the management to overcome the difficult economic conjecture, mainly in its domestic market: Portugal. The report is organized as a case-study. The first part, the case narrative, is subdivided into 6 parts, and the second part is the teaching note. The teaching note is constituted by the four questions and their respective responses.

1.° Magistri Tancredi de Corneto ordo judiciarius, libris quatuor : non pauca folia loco suo mota. — 2.° Ejusdem summa de matrimonio. — 3.° Novae constitutiones Frederici, Imperatoris

Mazarinaeus

1.° Martyrologium Usuardi : plurima folia loco suo mota sunt. — 2.° Isimberti, Abbatis sancti Martialis, littera, aliàs, redditus sive conquesta facta per Abbatem Isimbertum : sequuntur acta plurima confraternitatum. — 3.° Regula sancti Benedicti. — 4.° Lectiones de evangeliis per circulum anni, ad quarum marginem conjectae sunt variae litterae societatum. — 5.° Instrumenta conventionum ab Abbatibus monasterii Lemovicensi initarum. — 6.° Annotatio de eclipsi quae contigit idibus Septembris, anno 1178. — 7.° Alia instrumenta conventionum. — 8.° Catalogus librorum monasterii sancti Martialis. — 9.° Necrologium monasterii S. Martialis Lemovicensis. — Hujusce codicis pars duodecimo, pars decimo tertio saeculo videtur exarata.

Sancti Martialis Lemovicensis

1.° Martyrologium Usuardi : plurima folia loco suo mota sunt. — 2.° Isimberti, Abbatis sancti Martialis, littera, aliàs, redditus sive conquesta facta per Abbatem Isimbertum : sequuntur acta plurima confraternitatum. — 3.° Regula sancti Benedicti. — 4.° Lectiones de evangeliis per circulum anni, ad quarum marginem conjectae sunt variae litterae societatum. — 5.° Instrumenta conventionum ab Abbatibus monasterii Lemovicensi initarum. — 6.° Annotatio de eclipsi quae contigit idibus Septembris, anno 1178. — 7.° Alia instrumenta conventionum. — 8.° Catalogus librorum monasterii sancti Martialis. — 9.° Necrologium monasterii S. Martialis Lemovicensis. — Hujusce codicis pars duodecimo, pars decimo tertio saeculo videtur exarata.

Sancti Martialis Lemovicensis

historiae Longobardorum libri sex : initio et ad calcem occurrunt folia aliquot loco suo mota ; authoris nomen non comparet, is autem est Paulus Diaconus.

Faurianus

Interacciones entre 4 especies de hongos ectomicorrícicos y dos especies de coníferas del cerro El Potosí, Galeana, N.L., México por Rosa María Arias Mota

Tesis (Maestría en Ciencias Forestales) U.A.N.L.