Análise do Anexo I do Decreto nº 8.456/2015 relativa às despesas da área temática IV – MCTI, MEC, MinC e ME

Analisa e detalha as ações orçamentárias das dotações iniciais constantes da Lei Orçamentária para 2015 – LOA 2015 dos órgãos integrantes da Área Temática IV (Ministério da Ciência, Tecnologia e Inovação – MCTI, Ministério da Educação – MEC, Ministério da Cultura – MinC e Ministério do Esporte – ME) que foram objeto de limitação de movimentação e empenho em face do Decreto nº 8.456, de 22 de maio de 2015.

Seleção de sítio de divisão em Xanthomonas citri subsp. citri: caracterização de minC.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The C-terminal domain of MinC inhibits assembly of the Z ring in Escherichia coli.

In Escherichia coli, the Min system, consisting of three proteins, MinC, MinD, and MinE, negatively regulates FtsZ assembly at the cell poles, helping to ensure that the Z ring will assemble only at midcell. Of the three Min proteins, MinC is sufficient to inhibit Z-ring assembly. By binding to MinD, which is mostly localized at the membrane near the cell poles, MinC is sequestered away from the cell midpoint, increasing the probability of Z-ring assembly there. Previously, it has been shown that the two halves of MinC have two distinct functions. The N-terminal half is sufficient for inhibition of FtsZ assembly, whereas the C-terminal half of the protein is required for binding to MinD as well as to a component of the division septum. In this study, we discovered that overproduction of the C-terminal half of MinC (MinC(122-231)) could also inhibit cell division and that this inhibition was at the level of Z-ring disassembly and dependent on MinD. We also found that fusing green fluorescent protein to either the N-terminal end of MinC(122-231), the C terminus of full-length MinC, or the C terminus of MinC(122-231) perturbed MinC function, which may explain why cell division inhibition by MinC(122-231) was not detected previously. These results suggest that the C-terminal half of MinC has an additional function in the regulation of Z-ring assembly.