Sindhi-Jersey and Sindhi-Holstein crosses : their external form and internal anatomy compared with those of purebred Jerseys and Holsteins /

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Anatomy and histology of Embolyntha batesi MacLahlan, 1877 (Embiidina)

The Embioptera are rather generalized insects whose internal anatomy is simple and not subject to great modifications. For this reason these insects form an ideal group for elementary anatomical and histological studies (fig. 2). The digestive tract is a long, simple tube without convolutions or diverticulae from the buccal cavity to the rectum. The buccal structures are of the chewing type. The oesophagus and ingluvia are differentiated only by slight dilation of their walls. In nymphs and females the proventriculus is very distinct due to folds which flatten as the structure becomes packed with food. The enteron is the largest in such forms and in both sexes limited caudally by the Malpighian tubules. The proctodeus has six large rectal papillae. The nervous system is complete with only the fifth abdominal segment lacking a ganglion in the metathorax includes the ganglion of the first abdominal segment. The brain exhibits very clear structure in histological sections. The tracheal system includes two pairs of thoracic spiracles and eight abdominal pairs. Only th metathoracic spiracle has an air expiration function; all others serve for inspiration. Various structures in the spiracles protect the atrium. The circulatory system includes a long, simple dorsal vessel which extends forward from the ninth abdominal segment into the cranium. It opens anteriorly near the circumoesophageal connectives. The dorsal vessel has a pair of ostia and valves corresponding to each abdominal and thoracic segment. It lacks the diverticulae or folds commonly found in more highly evolved insects. The excretory system is represented by Malphighian tubules, pericardial cells, and fat-body. The number and disposition of Malpighian tubules is variable within the order. The pericardial cells are localized around the entire dorsal vessel up to the opening of the aorta in the head. The fat-bodies form compact layers in the dorsal and ventral regions of the body. In males they are more developed in the abdominal region. The mandibles, maxillae, and salivary glands are of a simple type with very few cytological modifications. Only the salivary glands which extend into the mesothoracic region show appreciable specialization. The reproductive system is bi-sixual and shows considerable sexual dimorphism. Males have five pair of testes with a metameric disposition, two distinct ducts, two epidymis, and the ejaculatory organs. The accessory glands vary in number and size and open in the anterior portion of the ejaculatory duct. The female reproductive organs are of the panoistic type. The system includes five pairs of ovarioles, two long paired oviducts a small, unpaired oviduct and the spermatheca which opens in the vagina. Reproduction usually involves a union of male and female gametes, and eggs are usually laid in clusters attached to a substrate.

A new species of sand-dwelling catfish, with a phylogenetic diagnosis of Pygidianops Myers (Siluriformes: Trichomycteridae: Glanapteryginae)

A new species of sand-dwelling catfish genus Pygidianops, P. amphioxus, is described from the Negro and lower Amazon basins. The new species differs from its three congeners in the elongate eel-like body, the short barbels, and the small caudal fin, continuous with the body, among other traits of internal anatomy. The absence of anal fin further distinguishes P. amphioxus from all other Pygidianops species except P. magoi and the presence of eyes from all except P. cuao. The new Pygidianops seems to be the sister species to P. magoi, the two species sharing a unique mesethmoid with a dorsally-bent tip lacking cornua, and a produced articular process in the palatine for the articulation with the neurocranium. Pygidianops amphioxus is a permanent and highly-specialized inhabitant of psammic environments. Additional characters are proposed as synapomorphies of Pygidianops, including a hypertrophied symphyseal joint and associated ligament in the lower jaw; an elongate, laterally-directed, process on the dorsal surface of the premaxilla; and a rotated lower jaw, where the surface normally facing laterally in other glanapterygines is instead directed ventrally. These and other characters are incorporated into a revised phylogenetic diagnosis of Pygidianops.

A new species of Trichogenes from the rio Itapemirim drainage, southeastern Brazil, with comments on the monophyly of the genus (Siluriformes: Trichomycteridae)

A new species of the formerly monotypic genus Trichogenes is described from a high-altitude stream of the rio Itapemirim system, an isolated Atlantic drainage in the State of Espírito Santo, southeastern Brazil. Trichogenes claviger, new species, differs from all other trichomycterids by the sexually dimorphic posterior process of the opercle, much elongated in males; the terminal mouth; the deeply bifurcated anterior neural spines and the presence of a large anterodorsal claw-like process on the neural arches of the anterior four free vertebrae. The new species also differs from its only congener, T. longipinnis, by a number of additional traits, including the the lack of branched anal-fin rays in specimens of any size; the broader than long posterior nostril; the deeper head (head depth 72.9-86.6% HL); the presence of a fine dark line along the base of the anal fin; the lack of dark spots on cheeks; the shape of the interopercle; the presence of odontodes on a bony expansion on the posterodorsal margin of the interopercle; the fewer vertebrae (35); the absence of an antorbital; and the fewer pleural ribs (eight). Small juveniles of the new species are also strikingly different from those of all other Trichomycteridae, including T. longipinnis, having a very large lateral eye, an upturned mouth, and compressed head. Trichogenes claviger occurs in shaded sectors of a blackwater sluggish stream with sandy substrate and patchy accumulations of vegetable debris, a habitat markedly different from the rocky torrential environment known for T. longipinnis. A comparison of the internal anatomy of the two species provides the basis for a hypothesis of a monophyletic Trichogenes. Data from the new species further support a sister-group relationship between Trichogeninae and Copionodontinae, as well as the position of that clade as sister group to all remaining Trichomycteridae.

Estudi de l'organogènesi del sistema reproductor de la cabra de mar (Maja brachydactyla) i efecte dels factors ambientals sobre la reproducció en captivitat

Diferents aspectes de la reproducció de la cabra de mar Maja brachydactyla s’han estudiat amb l’objectiu d’obtenir coneixements bàsics i aplicats al cultiu en captivitat d’aquesta espècie de gran interès comercial i aqüícola. L’anatomia interna de l’aparell reproductor masculí, el procés d’espermatogènesi, amb especial èmfasi amb la formació de l’acrosoma, i l’estructura i organització l’espermatozoide s’han descrit amb tècniques microscòpiques avançades. El desenvolupament de les gònades durant els primeres fases del desenvolupament post- embrionari (fases larvàries i primer cranc juvenil) han estat descrites mitjançant la quantificació de l’expressió del gen vasa, el qual és expressat específicament per les cèl•lules de la línia germinal. Aquests coneixements són bàsics per a la comprensió del paper dels mascles en la la reproducció i la seva aplicació posterior en condicions de captivitat. La reproducció en captivitat s’ha estudiat amb una sèrie d’experiments amb l’objectiu de determinar la quantitat, pes sec i composició bioquímica proximal de les larves acabades d’eclosionar en condicions de captivitat i l’efecte de les condicions ambientals, com ara el fotoperíode i la salinitat, sobre aquests paràmetres. Aquests experiments demostren què es poden obtenir larves de la cabra de mar en condicions de captivitat què podrien ser utilitzades per a la producció i l’efecte sobre la reproducció del fotoperíode i salinitat.

Enhanced Reality Visualization in a Surgical Environment

Enhanced reality visualization is the process of enhancing an image by adding to it information which is not present in the original image. A wide variety of information can be added to an image ranging from hidden lines or surfaces to textual or iconic data about a particular part of the image. Enhanced reality visualization is particularly well suited to neurosurgery. By rendering brain structures which are not visible, at the correct location in an image of a patient's head, the surgeon is essentially provided with X-ray vision. He can visualize the spatial relationship between brain structures before he performs a craniotomy and during the surgery he can see what's under the next layer before he cuts through. Given a video image of the patient and a three dimensional model of the patient's brain the problem enhanced reality visualization faces is to render the model from the correct viewpoint and overlay it on the original image. The relationship between the coordinate frames of the patient, the patient's internal anatomy scans and the image plane of the camera observing the patient must be established. This problem is closely related to the camera calibration problem. This report presents a new approach to finding this relationship and develops a system for performing enhanced reality visualization in a surgical environment. Immediately prior to surgery a few circular fiducials are placed near the surgical site. An initial registration of video and internal data is performed using a laser scanner. Following this, our method is fully automatic, runs in nearly real-time, is accurate to within a pixel, allows both patient and camera motion, automatically corrects for changes to the internal camera parameters (focal length, focus, aperture, etc.) and requires only a single image.

General morphology and ultrastructure of the venom apparatus and convoluted gland of the fire ant, Solenopsis saevissima

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biologia da formiga urbana Monomorium floricola Jerdon (Hymenoptera, Formicidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Biologia, morfologia e bioquímica de veneno da formiga lava-pés Solenopsis saevissima Smith (Insecta, Hymenoptera, Formicidae)

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Unusual labial glands in snakes of the genus Geophis Wagler, 1830 ( Serpentes: Dipsadinae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da anatomia interna dos incisivos laterais superiores pelos métodos radiográficos digital e convencional

The maxillary lateral incisor has a very peculiar and internal morphology that can be influenced by the its external anatomy such as supernumerary roots and ruts deep root, which often hinders an effective endodontic treatment. Generally, this tooth have a single root with one root canal, but teeth with two to four channels can be found. The main canal can present ramifications such as accessory canals, laterals, collaterals, recorrents and apical deltas. All this factors interfere directally on endodonthics treatment. The objective of this work is a study of the internal anatomy of the maxillary lateral incisors by digital and conventional radiographics methods, comparing the results obteined by each method Six hundred upper lateral incisor were used in this study teeth of the collection of the Department of Anatomy ICT – UNESP. All the teeth were radiographed by vestibular face, using a digital radiographic unit(Dabi Atlante). After this step, all the teeth underwent by conventional radiographic technique(Kodak 2200) so that the results obtained were compared. To classify the channel system, it was adopted the classification of Pucci and Reig. All data obtained by digital technique were compared with those obtained in the conventional technique and were subjected to statistical analysis

20 movies of x-ray tomograms of different Alpinia fossils

The fossil floras described by Dieter MAI and Harald WALTHER are invaluable for understanding the past plant diversity in Europe, and provide important information on the occurrence of taxa in the fossil record that is critical for evolutionary studies. Among the taxa they recognized were seeds assigned to the extant genus Alpinia ROXB. (Zingiberaceae, Zingiberales). We reinvestigated 28 specimens that were assigned to Alpinia arnensis (CHANDLER) MAI, Alpinia cf. arnensis, and Alpinia bivascularis MAI from the Ypresian (lower Eocene) of the UK, upper Eocene of Germany, and lower Miocene of Germany using non-destructive synchrotron-based X-ray tomography to reveal internal anatomy. None of the samples studied show an anatomy consistent with extant Alpinia or even Zingiberales. The fossils lack the globose shape, often striate external surface, seed coat structure, operculum, and micropylar collar seen in all Alpinia, and lack the chalazal chamber seen in many Alpinia species. Two specimens from the lower Miocene of Germany showed the structure of fruits of Caricoidea CHANDLER (Cyperaceae) with a single-layered exocarp, thick mesocarp, and sclerified endocarp. The other specimens are recognized as Carpolithes albolutum nom. nov. (incertae sedis) from the Ypresian of the UK, C. phoenixnordensis sp. nov. (incertae sedis) from the upper Eocene of Germany, C. bivascularis comb. nov. (incertae sedis) from the lower Miocene of Germany as well as indeterminate tegmens from the lower Miocene of Germany. This reinvestigation demonstrates that there is, as yet, no confirmed fossil record for the extant genus Alpinia. Furthermore, at least four different taxa are recognized from what had been two extinct species, enhancing our understanding of these important European Cenozoic carpofloras.

Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene

The formation of estrogens from C19 steroids is catalyzed by aromatase cytochrome P450 (P450arom), the product of the cyp19 gene. The actions of estrogen include dimorphic anatomical, functional, and behavioral effects on the development of both males and females, considerations that prompted us to examine the consequences of deficiency of aromatase activity in mice. Mice lacking a functional aromatase enzyme (ArKO) were generated by targeted disruption of the cyp19 gene. Male and female ArKO mice were born with the expected Mendelian frequency from F1 parents and grew to adulthood. Female ArKO mice at 9 weeks of age displayed underdeveloped external genitalia and uteri. Ovaries contained numerous follicles with abundant granulosa cells and evidence of antrum formation that appeared arrested before ovulation. No corpora lutea were present. Additionally the stroma were hyperplastic with structures that appeared to be atretic follicles. Development of the mammary glands approximated that of a prepubertal female. Examination of male ArKO mice of the same age revealed essentially normal internal anatomy but with enlargement of the male accessory sex glands because of increased content of secreted material. The testes appeared normal. Male ArKO mice are capable of breeding and produce litters of approximately average size. Whereas serum estradiol levels were at the limit of detection, testosterone levels were elevated, as were the levels of follicle-stimulating hormone and luteinizing hormone. The phenotype of these animals differs markedly from that of the previously reported ERKO mice, in which the estrogen receptor α is deleted by targeted disruption.

An unusual pliosaur (Reptilia, Sauropterygia) from the Kimmeridgian (Upper Jurassic) of northeastern Mexico

We here describe the rostrum of a pliosaur from the Kimmeridgian of northeastern Mexico. The specimen comes from the Upper Jurassic La Casita Formation (Kimmeridgian - Tithonian) and represents one of the few Plesiosauria in the area. The internal anatomy of the specimen is partly visible through cross-sections, which reveal a hitherto undescribed rostral prolongation of a paired bone of the interorbital area, probably the parietal or frontal beneath the premaxillae. The specimen also provides new information on the morphology and function of the choanae. In light of these morphological data, the new pliosaur shows similarities both with the European Late Jurassic genus Pliosaurus and the Australian Early Cretaceous Kronosaurus.