Iowa Airport Directory, 2003 ***NOTE*** This document is for historical viewing, the internal information is no longer current or accurate! ***NOTE*** Current information can be found at http://www.iowadot.gov/aviation/aircraftregistration/registration.aspx ***NOTE***

Iowa has 8 commercial service airports and 105 general aviation airports, of which three serve as reliever airports. ***NOTE*** This document is for historical viewing, the internal information is no longer current or accurate! ***NOTE*** Current information can be found at http://www.iowadot.gov/aviation/aircraftregistration/registration.aspx ***NOTE***

Mechanism of tocopherol transfer by human α-tocopherol transfer protein (α-hTTP)

Vitamin E is a well known fat soluble chain breaking antioxidant. It is a general tenn used to describe a family of eight stereoisomers of tocopherols. Selective retention of a-tocopherol in the human circulation system is regulated by the a -Tocopherol Transfer Protein (a-TIP). Using a fluorescently labelled a-tocopherol (NBD-a-Toc) synthesized in our laboratory, a fluorescence resonance energy transfer (FRET) assay was developed to monitor the kinetics of ligand transfer by a-hTTP in lipid vesicles. Preliminary results implied that NBD-a-Toe simply diffused from 6-His-a-hTTP to acceptor membranes since the kinetics of transfer were not responsive to a variety of conditions tested. After a series of trouble shooting experiments, we identified a minor contaminant, E coli. outer membrane porin F (OmpF) that co-purified with 6-His-a-hTTP from the metal affinity column as the source of the problem. In order to completely avoid OmpF contamination, a GST -a-hTTP fusion protein was purified from a glutathione agarose column followed by an on-column thrombin digestion to remove the GST tag. We then demonstrated that a-hTTP utilizes a collisional mechanism to deliver its ligand. Furthennore, a higher rate of a-tocopherol transfer to small unilamellar vesicles (SUV s) versus large unilamellar vesicles (LUV s) indicated that transfer is sensitive to membrane curvature. These findings suggest that ahTTP mediated a-Toc transfer is dominated by the hydrophobic nature of a-hTTP and the packing density of phospholipid head groups within acceptor membranes. Based on the calculated free energy change (dG) when a protein is transferred from water to the lipid bilayer, a model was generated to predict the orientation of a-hTTP when it interacts with lipid membranes. Guided by this model, several hydrophobic residues expected to penetrate deeply into the bilayer hydrophobic core, were mutated to either aspartate or alanine. Utilizing dual polarization interferometry and size exclusion vesicle binding assays, we identified the key residues for membrane binding to be F 165, F 169 and 1202. In addition, the rates of ligand transfer of the u-TTP mutants were directly correlated to their membrane binding capabilities, indicating that membrane binding was likely the rate limiting step in u-TTP mediated transfer of u-Toc. The propensity of u-TTP for highly curved membrane provides a connection to its colocalization with u-Toc in late endosomes.

Renssellaer Institute. Jim Hendler. CSCI 4964/COMM 49652 – Web Science. URIs and HTTP.

Slides on URIs and the HTTP protocol

A sample visualization of contributions to http://eprints.soton.ac.uk

This is a sample visualization of contributions to eprints.soton.ac.uk. The visualization was created from data from the OAI endpoint using gource. The data is divided by dc:subject classification. The idea was taken from Martin Hawksey's blog post http://mashe.hawksey.info/2011/12/google-refining-jorum-ukoer/

Web Architecture: HTTP Practical

A summary of the HTTP calls we made during the tutorial session.

El cuaderno de bitácora o blog : una herramienta informática para leer, reflexionar, escribir, opinar y comunicarse : http://gurrion.blogia.com.

El fenómeno de los blogs se ha extendido ampliamente gracias a su dinamismo y rapidez y por la facilidad de crearlos y de escribir en ellos. Permiten leer, reflexionar, expresarse, escribir, comunicarse y publicar. El profesor utiliza esta herramienta informática para colgar textos suyos o de los alumnos relacionados con situaciones de actualidad o con actividades que se hacen en clase, a los que Se ofrecen cinco textos y algunos comentarios a ellos como ejemplo de la experiencia.

Notifiche push in applicativi Android: uso di gcm ed una soluzione alternativa basata su long-polling http

Vengono presentate due soluzioni per le notifiche push in ambiente Android: le soluzioni trattate riguardano l'uso di GCM (Google Cloud Messaging) ed una implementazione che impiega il Long-Polling HTTP come alternativa al servizio GCM.