Influence of foliar fertilization with manganese on germination, vigor and storage time of RR soybean seeds

ABSTRACTThis study aimed to evaluate the influence of foliar fertilizer doses containing Mn of phenological stages of suggested application in RR soybeans, to recover management damages with glyphosate at postemergence application on seed vigor in post-harvest and post six months storage. The seeds originated from a field experiment conducted , which included two applications of glyphosate, concomitant with foliar fertilizer in growth stages V4 and V6, with 0.00, 113.50 and 227.00 mg ha-1doses of Mn2+. Germination, GSI (Germination Speed Index), electrical conductivity tests and the first count of seeds were conducted. The application of Mn did not affect the physiological quality of RR soy in postharvest. However, in post-storage, higher doses of Mn had a negative effect on tests of abnormal seedlings, GSI and electrical conductivity. The applications of Mn, regardless of the developmental stage, did not interfere in the germination and first count tests, with and without storage. The electrical conductivity test showed a higher correlation with the seed germination test in the post-harvest treatment.

Germination and vigor of Dimorphandra mollis benth. seeds under different temperatures and substrates

The Dimorphandra mollis Benth. - Caesalpiniaceae is a native forest species coming from the Cerrado and Caatinga due to its economical and ecological use, which justifies the studies on seed germination. In this work, germinative performance of D. mollis seeds were studied in different conditions of temperature regime and substrate. The experimental delineation used was completely randomized in factorial 4 x 4 (4 substrates -sand, coconut fiber, vermiculite and paper towel; and 4 temperatures: 25, 30, 35 and 20-30ºC), with four replications of 25 seeds each. The following parameters were evaluated: seed moisture content, final germination, first germination count, germination speed index, length and dry matter weight. The best germination and vigor is obtained at 30 and 35ºC. The substrates paper towel and vermiculite allow satisfactory germinative performance of seeds, being suitable to evaluate the physiological quality of D. mollis seeds.

TEMPERATURE AND SUBSTRATE INFLUENCES ON SEED GERMINATION AND SEEDLING FORMATION IN Callisthene fasciculata Mart. (VOCHYSIACEAE) IN THE LABORATORY1

ABSTRACTCallisthene fasciculata Mart. is a tree belonging to the Vochysiaceae family. Its wood is moderately heavy and resistant and used to make poles, beams, and other structures. The aim of this work was to evaluate seed germination and the initial growth of seedlings of C. fasciculata at different temperatures and in different substrates. Seeds were collected from fruits in the Pantanal de Miranda, Mato Grosso do Sul state, Brazil. In one experiment, the seeds were subjected to constant temperatures of 20, 25, 30 and 35 °C and to alternating temperatures of 20-30 and 25-35 °C (on paper substrate). In another experiment, the seeds were subjected to temperatures of 20 and 25 °C on three substrates (sand, vermiculite and between paper) in a germinator. The experiment had a randomized design, with four replicates of 25 seeds per treatment. The F-values obtained for germination indicated no significant effect of substrate or temperature on the final germination percentage. The analyses revealed no effect of a treatment interaction (temperature x substrate) on either germination or average germination time; however, a treatment interaction effect was observed on the germination speed index. The treatment combinations yielding the best performance were between paper substrate at 20 °C and sand substrate at 25 °C. There was a significant effect of the interaction between temperature and substrate on seedling growth, with increased root growth observed in the between paper substrate at 25 °C and increased aerial component growth in both sand at 20 °C and vermiculite at 25 °C. The between paper treatment at 25 °C yielded the greatest final seedling size. Between paper is the most recommended substrate for the production of seedlings due to its ease of handling and lower probability of contamination.

Germination and growth of Fimbristylis miliacea biotypes resistant and susceptible to acetolactate synthase-inhibiting herbicides

Weed biotypes resistant and susceptible to herbicides may have differences in their adaptive values. The aims of this study were to compare, under controlled and non-competitive condition, the growth analysis, germination features and seed weight of Fimbristylis miliacea (FIMMI) biotypes resistant and susceptible to acetolactate synthase (ALS) inhibiting herbicides. Experiments were conducted in a greenhouse and in a laboratory from October 2008 to February 2010. Two resistant biotypes (FIMMI 10 and FIMMI 12) and one susceptible biotype (FIMMI 13) were used for the studies. For the study on growth analysis, the treatments were arranged in a completely randomized experimental design with four replications and sampled at 21, 28, 35, 42, 49, 56, 69 days after emergence (DAE) and at flowering stage. For the studies on germination speed, germination and seed weight, the indexes for germination speed, percentage of germination at different temperatures and seed weight of the biotypes were determined. The results showed that the resistant biotype FIMMI 12 shows differences in all variables compared to the resistant biotype FIMMI 10 and compared to the susceptible biotype FIMMI 13, only for the evaluation at flowering. The susceptible biotype FIMMI 13 showed a higher germination speed index and higher germination rate when compared with the resistant biotypes. On the other hand, the resistant biotypes FIMMI 10 and FIMMI 12 showed higher seed weight.

Germination and Dormancy in Seeds of Sorghum halepense and Sorghum arundinaceum

Light, temperature and dormancy are factors that influence the germination of seeds and are strictly linked to the emergence of weeds. The objective of this work was to assess the germination of Sorghum arundinaceum and Sorghum halepense subjected to different conditions of temperature and luminosity, as well as assessing seed dormancy breaking mechanisms. For this, two experiments were conducted, both arranged in a completely randomized design. Experiment 1 was installed in a 2 x 5 double factorial design. The first factor was the absence or presence of light for 12 hours, and the other was composed of five constant temperatures: 15, 20, 30, 40 and 45 oC. In experiment 2, the efficiency of nine treatments used for breaking dormancy of seeds was assessed. The variables analyzed for both experiments were germination percentage and germination speed index (GSI). For the statistical analysis were performed an analysis of variance (ANOVA) and all the necessary consequences, as well as regression, when relevant. In experiment 1 for both species greater germination occurred in the presence of light. For S. arundinaceum the temperatures at which there was the highest percentage of germination were 33.13 and 31.24 oC for presence and absence of light respectively. As for S. halepense these temperatures were 31.98 and 29.75 oC for presence and absence of light respectively. As for the treatments for breaking dormancy, the mechanical scarification of seeds with sandpaper presented the highest germination and GSI. It is concluded that the Sorghum species studied are neutral photoblastic seeds and present mechanical type dormancy.

Drying and germination of cupuassu (Theobroma grandiflorum (willd. Ex Spreng.) K. Schum.) seeds

The objective of this study was to verify the effect of drying on germination of cupuassu (Theobroma grandiflorum (Willd. ex Spreng) K. Schum.) seeds. Desiccation was in forced air oven, with temperature ranging from 23 to 33ºC. Sowing was carried out at 0.5cm of depth in plastic trays in sand and sawdust mixture (1:1), previously sterilized in hot water (100ºC), during 2h. Seeds were left to germinate in a laboratory with no temperature and relative humidity control, under natural light. It was quantified the seed moisture content, in four replications of 10 seeds; the germination percentage, performed during 30 days, with daily counts of the number of germinated seeds; the germination speed index; and number of days to the germination onset. The experimental design was completely randomized with four replications of 25 seeds. The reduction of moisture content from 58.6 to 37.8% did not affect seed germination and germination speed index; however, they were affected when moisture content was reduced to values below 30.7%. It was observed that only when moisture content was 16.1% seeds demanded more days to begin germination. Cupuassu seeds are classified as recalcitrant and they can be desiccated up to 37.8% with no reduction on germination.

Effects of temperature and pre-germinative treatments on seed germination of Talinum triangulare (Jacq. ) willd (Portulacaceae)

The objective of this study was to evaluate the effects of temperature, substrate and pre-germinative treatments on T. triangulare seeds. Four temperatures (constant 20, 25, 30 °C and alternate 20-30 °C) and two types of seeding (on paper and between paper), with light, were evaluated. The pre-germinative treatments evaluated included: immersion in water (24 hours), immersion in 6% hypochlorite solution (1 hour), immersion in 0.2% potassium nitrate solution (24 hours), immersion in 0.05% gibberellin solution (24 hours) and the control (untreated seeds). The highest germination percentage was observed at the alternate temperatures of 20-30 ºC, but with no significant difference between the substrates at this temperature. Soaking seeds in KNO3 gave the highest percentage germination and germination speed index (GSI), which differed from the other treatments except for soaking in water. Pre-soaking of T. triangulare seeds favors germination and may be done only in pure water, resulting in a more rapid and uniform germination.

Mobilization of reserves and germination of seeds of Erythrina velutina Willd. (Leguminosae - Papilionoideae) under different osmotic potentials

Some environmental factors, including water availability, may influence seed germination. This study investigated the germination of E. velutina seeds submitted to different osmotic potentials and mobilization of reserves during water-stress. Scarified seeds were arranged in paper rolls and soaked in solutions of Polyethylene Glycol (PEG) prepared in osmotic potentials 0.0, -0.2, -0.4, -0.6, and -0.8 MPa and kept into a seed germinator, at 25 °C, and 12/12 h photoperiod (L/D), during 10 days. The percentage, mean time, mean speed, germination speed index; as well as the germination uniformity coefficient were assessed. During germination process the total soluble sugars, reducing sugars, soluble protein, and total amino acids were quantified in the cotyledon, hypocotyl and radicle of soaked seeds and cotyledons of quiescent seeds (control). There was influence of osmotic potential on E. velutina seed germination. The germination percentage remained at high levels until -0.6 MPa and above this osmotic potential there has been no germination. The mobilization of stored reserves of carbon and nitrogen in E. velutina seeds was also influenced by water-stress. There was sensitiveness between -0.2 and -0.6 MPa; however, the degradation and the mobilization of reserves was slower when the osmotic potential decreased.

Substrate and Temperature Effects on the Germination of Dypsis leptocheilos (Hodel) Beentje & J. Dransf. (Arecaceae) Seeds

Dypsis leptocheilos is highly valued as an ornamental palm. Its propagation is done by seeds; however, there is little information about this process. The objective of this work was to study the substrate and temperature effects on the germination of D. leptocheilos seeds. The experiments were carried out at FCAV/UNESP, Campus of Jaboticabal, São Paulo State, Brazil. In order to study the substrate effects, four treatments were arranged (coconut fiber, sand, vermiculite and Plantmax (R)) at 30 degrees C. For the temperature effects, six treatments were performed (temperature at laboratory conditions (21.5 degrees C and 72% RU), 25 degrees C, 30 degrees C, 35 degrees C, 20-30 degrees C and 25-35 degrees C), having the coconut fiber as the substrate. The experimental design was completely randomized, with four replicates of 25 disseminules per plot (seeds with attached endocarp). Water replacement was managed to maintain 100% of the retention capacity of the substrates. The germination tests were observed every two days, and conducted over 148 days for the substrate effects, and over 152 days for the temperature effects, when no more seed germination was noted. In order to determine the germination percentage (% G) and the Germination Speed Index (GSI), the seed was considered germinated when the germinative button appeared. At the end of the experiments, leaf area (cm(2)), root and shoot length (cm), root and shoot dry mass (g) were also obtained. Statistical analysis was performed and means were compared by the Tukey test. Germination rate and speed in coconut fiber was higher at 25 and 30 degrees C. However, when other substrates were tested at 30 degrees C, the highest germination percentage was observed in vermiculite, which also sustained better results for the seed germination and for the characteristics related to shoots and roots.

Morphology and initial growth in the germination of dictyosperma album (bory) h. wendl. & drude ex scheffer (Arecaceae) seeds

Dictyosperma album, commonly known as Princess Palm, due to its graceful appearance and its ability to withstand strong hurricane force winds is largely used in landscaping areas. The aim of this work was to describe the diaspores (seeds with the stuck endocarp) morphology and the stages of the D. album germination process. Therefore we concluded that the species germination is of the tubular remote type and begins with the opening of a circular operculum in the fruit endocarp, through which emerges a bulbous and hollow structure, named cotyledonal petiole or embryophore. With the embryo growth, the endosperm is being gradually consumed. The embryo grows approximately up to 10 cm, when the dilation begins in its extremity. Starting from this extensive area, it begins the growth of the primary root and the opening of a longitudinal rift, through which emerges the aerial part that is composed by two sheaths that cover the first juvenile leaf. In this phase, the emergence of secondary roots is observed. The first leaves are simple and lance-shaped.

Eco-physiological aspects of melaleuca seed germination

The invasive behavior of melaleuca (Melaleuca quinquenervia) plants in wetlands is due to its aggressive regeneration strategy, which is based on its seeds germination performance. Understanding of the eco-physiological aspects of the seed germination in melaleuca plants may significantly contribute for the development of management strategies. The objective of this research was to learn how the germination of M. quinquenervia seeds are affected by light and temperature. Melaleuca seeds were placed on filter paper moistened with 12 ml of distilled water at temperatures between 10 and 45°C. Germination was evaluated in dark and light conditions. Seed germination, first count of seed germination (seven days), germination speed index and germination mean time were determined up to 40 days after seeding, when germination had ceased in most of the treatments. After that period, the seeds were transferred to conditions of 30°C and light, which was found to be ideal in the previous phase. Seed germination was daily evaluated up to 63 days when it was again observed no longer to occur. The treatment repetitions were distributed in the growth-chamber according to a completely randomized design in a factorial scheme (eight temperatures x two light conditions) and four repetitions. The data were submitted to analysis of variance with the F test and the means were adjusted to polynomial and non linear regression models. The highest seed germination performance was observed to take place under conditions of 27.3°C with light. The temperatures of 35 and 40°C in the dark induced thermal inhibition of seed germination. The temperature of 45°C was lethal to the seeds.

Effects of the Temperature and Maturation Stages on the Germination of Roystonea regia Seeds

Roystonea regia (Kunth) O.F. Cook is largely used as ornamental. The propagation is done almost exclusively by seeds; however, there is a great variation in the germination process influenced by many factors. The objective of this work was to study the effects of the temperature and maturation stages on the germination of R. regia seeds. The experimental design was entirely randomized in a factorial arrangement 6x3 (six temperatures: constant at 20, 25, 30 and 35 degrees C and alternated at 20-30 and 25-35 degrees C, with a photoperiod of 12 hours; and three fruit maturation stages: brown, yellow and black), with four replications of 25 disseminules (seed with stucked endocarp) each. The disseminules had their mesocarp and exocarp were removed and shade dried. Their moisture content was determined, and then they were placed in plastic boxes (gerbox type) containing vermiculite. The disseminules, with the germinative intumescence, were daily noted until germination was steady. The germination rate and the germination speed index were calculated, and the data were submitted to the variance analysis. The means were compared by the Tukey test. It was concluded that the highest germination rate (99.7%) and germination speed were obtained by seeds from mature (black) fruits at the temperature of 35 degrees C.

Germination of Roystonea borinquena OF Cook (Arecaceae) Seeds

This work aimed to study the effect of the temperature on the seed germination and to describe the morphology and germination process of disseminules (seeds with stucked endocarp) of R. borinquena species. The fruits were harvested and had their pulp (exocarp and mesocarp) removed and the disseminules were shade dried. The experimental design was entirely randomized and six treatments were performed (temperature conditions: constant at 25, 30 and 35 degrees C; alternated at 25-35 and 20-30 degrees C; and room temperature) with four replications with 25 disseminules per plot, placed in plastic boxes (gerbox) containing vermiculite. The germination rate and germination speed index were calculated, and the data were submitted to the variance analysis, and the means compared by the Tukey test. For the morphological studies, the external and internal sides of the disseminules, the embryo and the representative germination stages were sketched. The samples were fixed in FAA (formalin - acetic acid - ethyl alcohol). It was concluded that the highest germination rate and faster germination were at the constant temperature of 35 degrees C and alternated of 25-35 degrees C. The seeds are albuminous, with a hard endosperm that takes almost the entire interior of the disseminules; the embryo is lateral, peripheral and poorly differentiated; the germination is adjacent.

Germination of Phoenix roebelenii Seeds

The Phoenix roebelenii O'Brien is one of the most used plants in the Brazilian landscape. The work aimed to study the effect of different fruit harvesting locations, associated with seven temperature conditions, on the germination of P. roebelenii seeds. The fruits were collected from two different locations: Ilha Solteira, western Sao Paulo State and Jaboticabal in the middle East of the same State. The disseminules (seeds with stucked endocarp) were placed in plastic boxes (gerbox), and the experimental design was entirely randomized in a factorial scheme of 7x2 (seven temperatures conditions: room temperature, constant at 20, 25, 30 and 35 degrees C and alternated at 20-30 and 25-35 degrees C; and two fruit harvesting locations: Ilha Solteira and Jaboticabal, with four replications with 25 seeds each. The disseminules that emitted the germinative intumescence were daily noted until the germinative process got steady, in about 90 days. The germination rate (G%), the germination speed index (GSI), and the means were extracted and compared by the Tukey test. The highest values of germination rate were obtained at 30, 25-35 degrees C and room temperatures. The seeds showed a higher GSI at room temperature. The fruit harvesting locations influenced the germination.

Speed Index in the Racing Quarter Horse: A Genome-wide Association Study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)