Suosittelulla lisää uusia asiakkaita

Asiakashankintaa tehdään monin tavoin. Suosittelua asiakashankinnan keinona on tutkittu tieteellisissä tutkimuksissa melko vähän. Tämän tutkimuksen tavoitteena on selvittää asiakashankinnan ja suosittelun nykytilaa kohdeorganisaatiossa, kehittää suosittelua osaksi myyntiprosessia ja tunnistaa aineettomia resursseja, joita voidaan hyödyntää asiakashankinnan ja suosittelun tehostamisessa tietojohtamisen avulla. Tutkimus on kvalitatiivinen tapaustutkimus ja sen tavoitteisiin pyritään vastaamaan survey kyselyn sekä puolistrukturoitujen haastatteluiden (14kpl) avulla. Tutkimuksen kohdeorganisaationa on Suomessa toimiva pankki- ja rahoitusalan yhtiö. Suosittelun käyttöä asiakashankinnan keinoa tutkittiin kohdeorganisaation yrityksen pankkineuvojien ja heidän esimiesten näkökulmasta. Empiirinen tutkimus antaa vastauksen asetettuihin tutkimuskysymyksiin sekä vastaa tutkimuksen tavoitteisiin. Tutkimuksessa löydettiin suositteluun ja yleisesti asiakashankintaan liittyviä aineettomia resursseja, joita hyödyntämällä ja johtamalla suosittelun nykyistä käyttöä voidaan kehittää tehokkaammaksi.

Gender Differences in Life Expectancy and Disability-Free Life Expectancy Among Older Adults in Sao Paulo, Brazil

Background: Research on life expectancy has demonstrated the negative impact of disability on the health of older adults and its differential effects on women as evidenced by their higher disabled life expectancy (DLE). The goal of the present study was to investigate gender differences in total life expectancy (TLE), disability-free life expectancy (DFLE), and DLE; examine gender differences on personal care assistance among older adults in Sao Paulo, Brazil; and discuss the implications for public policies. Methods: The sample was drawn from two waves (2000, 2006) of the dataset of Salud, Bienestar, y Envejecimiento, a large longitudinal study conducted in Sao Paulo (n = 2,143). The study assessed disability using the activities of daily living (ADL). The interpolation of Markov Chain method was used to estimate gender differences in TLE, DLE, and DFLE. Findings: TLE at age 60 years was approximately 5 years longer for women than men. Women aged 60 years were expected to live 28% of their remaining lives twice the percentage for men with at least one ADL disability. These women also lived more years (M = 0.71, SE = 0.42) with three or more ADL disabilities than men (M = 0.82, SE = 0.16). In terms of personal care assistance, women received more years of assistance than men. Conclusion: Among older adults in Sao Paulo, women lived longer lives but experienced a higher and more severe disability burden than men. In addition, although women received more years of personal assistance than men, women experienced more unmet care assistance needs. Copyright (C) 2011 by the Jacobs Institute of Women`s Health. Published by Elsevier. Inc.

Aplicação da teoria das restrições na gestão de projectos: caso em estudo

Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Mecânica - Manutenção e Produção

Conceitos de gestão de projetos e teoria das restrições, aplicados ao planejamento e controle da produção de uma empresa aeronaútica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modelo enxuto de gerenciamento multiprojetos baseado na corrente crítica

Pós-graduação em Engenharia Mecânica - FEG

Cutting knife assembly for a combine

A cutting knife assembly for a combine having an elongated platform with an auger means rotatably mounted thereon. The auger means has first and second helical fighting sections extending inwardly from its ends which are adapted to convey the cut crop to the center of the platform. A first endless chain is operatively positioned forwardly of the first flighting section and has a plurality of first cutting elements mounted thereon. A second endless chain is operatively positioned forwardly of the second flighting section and has a plurality of second cutting elements mounted thereon. The first and second chains are operated in opposing directions so that the first and second cutting elements move away from the center of the platform as the cutting elements cut the standing crop. The opposing action of the cutting elements causes the crop stems to be properly oriented with respect to the flighting sections so that the stems will be inclined towards the center of the platform in the same direction as the auger feed.

Contribuciones al análisis de problemas supercomplejos de toma de decisiones

Los fundamentos de la Teoría de la Decisión Bayesiana proporcionan un marco coherente en el que se pueden resolver los problemas de toma de decisiones. La creciente disponibilidad de ordenadores potentes está llevando a tratar problemas cada vez más complejos con numerosas fuentes de incertidumbre multidimensionales; varios objetivos conflictivos; preferencias, metas y creencias cambiantes en el tiempo y distintos grupos afectados por las decisiones. Estos factores, a su vez, exigen mejores herramientas de representación de problemas; imponen fuertes restricciones cognitivas sobre los decisores y conllevan difíciles problemas computacionales. Esta tesis tratará estos tres aspectos. En el Capítulo 1, proporcionamos una revisión crítica de los principales métodos gráficos de representación y resolución de problemas, concluyendo con algunas recomendaciones fundamentales y generalizaciones. Nuestro segundo comentario nos lleva a estudiar tales métodos cuando sólo disponemos de información parcial sobre las preferencias y creencias del decisor. En el Capítulo 2, estudiamos este problema cuando empleamos diagramas de influencia (DI). Damos un algoritmo para calcular las soluciones no dominadas en un DI y analizamos varios conceptos de solución ad hoc. El último aspecto se estudia en los Capítulos 3 y 4. Motivado por una aplicación de gestión de embalses, introducimos un método heurístico para resolver problemas de decisión secuenciales. Como muestra resultados muy buenos, extendemos la idea a problemas secuenciales generales y cuantificamos su bondad. Exploramos después en varias direcciones la aplicación de métodos de simulación al Análisis de Decisiones. Introducimos primero métodos de Monte Cario para aproximar el conjunto no dominado en problemas continuos. Después, proporcionamos un método de Monte Cario basado en cadenas de Markov para problemas con información completa con estructura general: las decisiones y las variables aleatorias pueden ser continuas, y la función de utilidad puede ser arbitraria. Nuestro esquema es aplicable a muchos problemas modelizados como DI. Finalizamos con un capítulo de conclusiones y problemas abiertos.---ABSTRACT---The foundations of Bayesian Decisión Theory provide a coherent framework in which decisión making problems may be solved. With the advent of powerful computers and given the many challenging problems we face, we are gradually attempting to solve more and more complex decisión making problems with high and multidimensional uncertainty, múltiple objectives, influence of time over decisión tasks and influence over many groups. These complexity factors demand better representation tools for decisión making problems; place strong cognitive demands on the decison maker judgements; and lead to involved computational problems. This thesis will deal with these three topics. In recent years, many representation tools have been developed for decisión making problems. In Chapter 1, we provide a critical review of most of them and conclude with recommendations and generalisations. Given our second query, we could wonder how may we deal with those representation tools when there is only partial information. In Chapter 2, we find out how to deal with such a problem when it is structured as an influence diagram (ID). We give an algorithm to compute nondominated solutions in ID's and analyse several ad hoc solution concepts.- The last issue is studied in Chapters 3 and 4. In a reservoir management case study, we have introduced a heuristic method for solving sequential decisión making problems. Since it shows very good performance, we extend the idea to general problems and quantify its goodness. We explore then in several directions the application of simulation based methods to Decisión Analysis. We first introduce Monte Cario methods to approximate the nondominated set in continuous problems. Then, we provide a Monte Cario Markov Chain method for problems under total information with general structure: decisions and random variables may be continuous, and the utility function may be arbitrary. Our scheme is applicable to many problems modeled as IDs. We conclude with discussions and several open problems.

Hough Transform Approach to Identification of Flare Stars in Multi-exposure Plate Images

ACM Computing Classification System (1998): I.2.8 , I.2.10, I.5.1, J.2.

A simplified minimal residual disease polymerase chain reaction method at early treatment points can stratify children with acute lymphoblastic leukemia into good and poor outcome groups

Background Minimal residual disease is an important independent prognostic factor in childhood acute lymphoblastic leukemia. The classical detection methods such as multiparameter flow cytometry and real-time quantitative polymerase chain reaction analysis are expensive, time-consuming and complex, and require considerable technical expertise. Design and Methods We analyzed 229 consecutive children with acute lymphoblastic leukemia treated according to the GBTLI-99 protocol at three different Brazilian centers. Minimal residual disease was analyzed in bone marrow samples at diagnosis and on days 14 and 28 by conventional homo/heteroduplex polymerase chain reaction using a simplified approach with consensus primers for IG and TCR gene rearrangements. Results At least one marker was detected by polymerase chain reaction in 96.4%, of the patients. By combining the minimal residual disease results obtained on days 14 and 28, three different prognostic groups were identified: minimal residual disease negative on days 14 and 28, positive on day 14/negative on day 28, and positive on both. Five-year event-free survival rates were 85%, 75.6%,, and 27.8%, respectively (p<0.0001). The same pattern of stratification held true for the group of intensively treated children. When analyzed in other subgroups of patients such as those at standard and high risk at diagnosis, those with positive B-derived CD10, patients positive for the TEL/AML1 transcript, and patients in morphological remission on a day 28 marrow, the event-free survival rate was found to be significantly lower in patients with positive minimal residual disease on day 28. Multivariate analysis demonstrated that the detection of minimal residual disease on day 28 is the most significant prognostic factor. Conclusions This simplified strategy for detection of minimal residual disease was feasible, reproducible, cheaper and simpler when compared with other methods, and allowed powerful discrimination between children with acute lymphoblastic leukemia with a good and poor outcome.

Detection and identification of dengue virus isolates from Brazil by a simplified reverse transcription - polymerase chain reaction (RT-PCR) method

We show here a simplified RT-PCR for identification of dengue virus types 1 and 2. Five dengue virus strains, isolated from Brazilian patients, and yellow fever vaccine 17DD as a negative control, were used in this study. C6/36 cells were infected and supernatants were collected after 7 days. The RT-PCR, done in a single reaction vessel, was carried out following a 1/10 dilution of virus in distilled water or in a detergent mixture containing Nonidet P40. The 50 µl assay reaction mixture included 50 pmol of specific primers amplifying a 482 base pair sequence for dengue type 1 and 210 base pair sequence for dengue type 2. In other assays, we used dengue virus consensus primers having maximum sequence similarity to the four serotypes, amplifying a 511 base pair sequence. The reaction mixture also contained 0.1 mM of the four deoxynucleoside triphosphates, 7.5 U of reverse transcriptase, 1U of thermostable Taq DNA polymerase. The mixture was incubated for 5 minutes at 37ºC for reverse transcription followed by 30 cycles of two-step PCR amplification (92ºC for 60 seconds, 53ºC for 60 seconds) with slow temperature increment. The PCR products were subjected to 1.7% agarose gel electrophoresis and visualized by UV light after staining with ethidium bromide solution. Low virus titer around 10 3, 6 TCID50/ml was detected by RT-PCR for dengue type 1. Specific DNA amplification was observed with all the Brazilian dengue strains by using dengue virus consensus primers. As compared to other RT-PCRs, this assay is less laborious, done in a shorter time, and has reduced risk of contamination

Polymerase chain reaction-based method for the identification of Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in mucosal tissues conserved in paraffin

ABSTRACTINTRODUCTION: In the Americas, mucosal leishmaniasis is primarily associated with infection by Leishmania (Viannia) braziliensis. However, Leishmania (Viannia) guyanensis is another important cause of this disease in the Brazilian Amazon. In this study, we aimed at detecting Leishmaniadeoxyribonucleic acid (DNA) within paraffin-embedded fragments of mucosal tissues, and characterizing the infecting parasite species.METHODS: We evaluated samples collected from 114 patients treated at a reference center in the Brazilian Amazon by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses.RESULTS: Direct examination of biopsy imprints detected parasites in 10 of the 114 samples, while evaluation of hematoxylin and eosin-stained slides detected amastigotes in an additional 17 samples. Meanwhile, 31/114 samples (27.2%) were positive for Leishmania spp. kinetoplast deoxyribonucleic acid (kDNA) by PCR analysis. Of these, 17 (54.8%) yielded amplification of the mini-exon PCR target, thereby allowing for PCR-RFLP-based identification. Six of the samples were identified as L. (V.) braziliensis, while the remaining 11 were identified as L. (V.) guyanensis.CONCLUSIONS: The results of this study demonstrate the feasibility of applying molecular techniques for the diagnosis of human parasites within paraffin-embedded tissues. Moreover, our findings confirm that L. (V.) guyanensisis a relevant causative agent of mucosal leishmaniasis in the Brazilian Amazon.

A simplified method for sample collection and DNA isolation for polymerase chain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors

Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America, sharing several reservoirs and triatomine vectors, we herein describe a simple method to collect triatomine feces and hemolymph in filter paper for further detection and specific characterization of these two trypanosomes. Experimentally infected triatomines feces and hemolymph were collected in filter paper and specific detection of T. rangeli or T. cruzi DNA by polymerase chain reaction was achieved. This simple DNA collection method allows sample collection in the field and further specific trypanosome detection and characterization in the laboratory.

Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains

Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.