Intrahospital spread of carbapenem-resistant Pseudomonas aeruginosa in a University Hospital in Florianópolis, Santa Catarina, Brazil

INTRODUCTION: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) has been isolated with increasing frequency in Brazilian hospitals. Since June 2003, its detection in a teaching hospital in the city of Florianópolis, Brazil, has increased. This study aimed to investigate the minimal inhibitory concentration (MIC), presence of Metallo-β-lactamase (MβL) and a possible clonal relationship among the isolates. METHODS: The study included 29 CRPA and seven isolates with reduced susceptibility. The MIC was determined by agar-dilution. Detection of MβL was performed by Double Disk Sinergism (DDS) and Combined Disk (CD). The MβL gene was verified by PCR and nucleotide sequence analysis. Epidemiological typing was performed by pulsed-field gel electrophoresis. RESULTS: Among the 29 carbapenem-resistant isolates, polymyxin B presented 100% susceptibility and piperacillin/tazobactam 96.7%. Seventeen (62%) strains were verified as clonal (A clone) and among these, six isolates indicated phenotypically positive tests for MβL and harbored the blaSPM-1 gene. The first CRPA isolates were unrelated to clone A, harbored blaIMP-16 and were phenotypically positive only by CD. CONCLUSIONS: The spread of a high-level of resistance clone suggests cross transmission as an important dissemination mechanism and has contributed to the increased rate of resistance to carbapenems. This study emphasizes the need for continuous surveillance and improved strategies.

Fibrinolytic therapy for thrombosis in cardiac valvular prosthesis short and long term results

OBJECTIVE: To assess the short- and long-term results of the use of streptokinase (SK) for the treatment of thromboses in cardiac valvular prostheses. METHODS: Seventeen patients with cardiac prosthetic thrombosis diagnosed by clinical, echocardiographic, and radioscopic findings underwent fibrinolytic treatment with a streptokinase bolus of 250,000 U followed by 100.000 U/hour. Short- and long-term results were assessed by radioscopy and echocardiography. RESULTS: Of the 17 patients, 12 had mechanical double-disk prostheses (4 aortic, 6 mitral, 2 tricuspid), 4 had single-disk prostheses (2 aortic, 1 mitral, and 1 tricuspid), and 1 had a tricuspid bioprosthesis. The success rate was 64.8%, the partial success rate was 17.6%, and the nonsuccess rate was 17.6%. All patients with a double-disk prosthesis responded, completely or partially, to the treatment. None of the patients with a single-disk prosthesis had complete resolution of the thrombosis. The time of streptokinase infusion ranged from 6 to 80 hours (mean of 56 h). The mortality rate due to the use of streptokinase was 5.8% and was secondary to cerebral bleeding. During streptokinase infusion, 3 (17.6%) embolic episodes occurred as follows: 1 cerebral, 1 peripheral, and 1 coronary. The rethrombosis index was 33% in a mean follow-up of 42 months. CONCLUSION: The use of fibrinolytic agents was effective and relatively safe in patients with primary thrombosis of a double-disk prosthesis. A fatal hemorrhagic complication occurred in 1 (5.8%) patient, and embolic complications occurred in 3 (17.6%) patients. In a mean 42-month follow-up, 67% of the patients were free from rethrombosis.

Oxisol resistence to penetration in no-till system after sowing

If inappropriately conducted, management and sowing practices may compromise the environment and the profitability of the agricultural activity. The aim of this study was to analyze the furrow opener mechanisms action and the level of load applied to soil firming mechanism in no-till, on the Oxisol resistance to penetration during soybean sowing, under three soil moistures. The experiment was arranged in split-split plot design, in which the plots were composed by three soil moistures (23.8; 25.5 and 27.5% b.s.), two furrow opener mechanisms sub-plots (double disks and furrow plough) and the split-split plot of three levels of load applied to soil firming mechanism (12.2; 18.5 and 24.1 kPa), according to randomized blocks design, with three replications. The soil moisture provided different resistance behavior to penetration with the depth, on the seedbed, independently of the furrow opener and the level of load applied to soil firming mechanism. The furrow plough use provided less soil resistance to penetration when compared to the double disk furrow opener, on the seedbed, independently of the soil moisture and the level of load applied to soil firming mechanism. The pressure applied by soil firming mechanism of 18.5 kPa provided the lower resistance to penetration, when the furrow plough was used. The soil resistance to penetration was less on the sowing line than on between rows, with 20 cm deep.

Molecular epidemiology and extended-spectrum β-lactamases production of Klebsiella pneumoniae isolated from three dairy herds

The objectives of this study were to isolate Klebsiella pneumoniae from different sources in three dairy cattle herds, to use the pulsed-field gel electrophoresis (PFGE) to measure genotypic similarities between isolates within a dairy herd, to verify the production of extended-spectrum β-lactamases (ESBLs) by the double-disk synergy test (DDST), and to use the PCR to detect the main ESBLs subgroups genes. Three dairy farms were selected based on previous mastitis outbreaks caused by K. pneumoniae. Milk samples were collected from lactating cows and from the bulk tank. Swabs were performed in different locations, including milking parlors, waiting room, soil, animal's hind limbs and rectum. K. pneumoniae was isolated from 27 cases of intramammary infections (IMI) and from 41 swabs. For farm A isolates from IMI and bulk tank were considered of the same PGFE subtype. One isolate from a bulk tank, three from IMI cases and four from environmental samples were positive in the DDST test. All eight DDST positive isolates harbored the bla shv gene, one harbored the bla tem gene, and three harbored the bla ctx-m gene, including the bulk tank isolate. Our study confirms that ESBL producing bacteria is present in different locations in dairy farms, and may be responsible for IMI. The detection of ESBLs on dairy herds could be a major concern for both public and animal health.

Análise tridimensional de esforços em elementos rompedores de semeadoras de plantio direto

As informações da avaliação de mecanismos de abertura de sulco de semeadoras de plantio direto auxiliam as empresas no dimensionamento de ferramentas rompedoras do solo, que buscam menor requerimento energético. Este trabalho foi realizado num solo classificado como Latossolo Vermelho com o objetivo de avaliar tridimensionalmente os esforços dos mecanismos de abertura de sulco de semeadoras adubadoras de plantio direto. O delineamento experimental utilizado foi o de blocos ao acaso com cinco tratamentos, sendo cinco mecanismos de abertura de sulco (disco liso - DL, disco corrugado - DC, disco ondulado - DO, disco duplo - DD e haste sulcadora - H). Para mensurar os esforços dos mecanismos de abertura de sulco, foi utilizado um dinamômetro com sensibilidade nas direções horizontal, transversal e vertical e no momento fletor e torsor. A avaliação mostrou que a interação entre mecanismos de abertura de sulco e carga vertical não foi significativa, entretanto os fatores foram significativos quando analisados individualmente. O resultado individual dos mecanismos de abertura de sulco mostrou que o disco ondulado proporcionou maior área de mobilização do solo, a haste sulcadora apresentou o menor valor de força vertical e o disco duplo e a haste sulcadora obtiveram o menor esforço horizontal.

Esforços em ferramentas rompedoras de solo de semeadoras de plantio direto

The individual evaluation of mechanisms for opening furrows to drop the seeds in a no-tillage seeders cultivation assists the companies in the sizing of opening tools of the ground, that search for minor energy petition and for diminishing the ground area mobilized. Therefore this work had the aim of evaluate the efforts of five different mechanisms of opening of ridge of seeding of direct plantation varyng the vertical load. The experiment was installed in one ground classified as Dystroferric Red Nitosol. The used experimental delineation was of the one randomized blocks in a factorial project 5 x 4, constituted a the following factors: five mechanisms of furrow opening (even disk - DL, corrugated disk - DC, ondulated disk - DO, double disk - DD and furrow rod - F) and four vertical loads (750, 1500, 2250 and 3000 N). To measure the forces in the studied treatments a dynamometer with sensitivity in the directions was used horizontal, lateral and vertical direction. The evaluation showed that the interaction between mechanisms of opening of ridge and vertical load was not significant, however the factors were significant when analyzed individually. The connecting rod promoted mobilization of the ground with smaller draft of horizontal effort reaching depths of work. The work showed that the vertical load influenced the depth of work of the mechanisms of ridge opening. The mechanisms of ridge opening of record type need vertical force to penetrate into the ground.

Fertilizer distribution mechanisms and side dress nitrogen fertilization in upland rice under no-tillage system

Algumas culturas têm pouca adaptação ao sistema plantio direto (SPD), em vista da alta compactação da camada superficial do solo. Nesse caso, o mecanismo utilizado na semeadora para a abertura dos sulcos para deposição do fertilizante pode ter grande importância no sentido de facilitar a penetração das raízes. Avaliouse a influência do mecanismo de distribuição de fertilizante e da adubação nitrogenada na cultura do arroz de terras altas (Oryza sativa) no sistema plantio direto. O experimento foi conduzido nos anos agrícolas 2001/2002 e 2002/2003, em Botucatu-SP, Brasil. O delineamento experimental foi em blocos casualizados, com parcelas subdivididas e quatro repetições. As parcelas foram constituídas por dois mecanismos de distribuição de fertilizantes (haste sulcadora e disco duplo). Nas subparcelas, quatro níveis de N foram aplicados em cobertura (0, 40, 80 e 120 kg ha-1). Avaliou-se a profundidade de abertura do sulco e de deposição das sementes, a população de plantas, a altura de plantas, o número de colmos e de panículas m-2, o número total de espiguetas por panícula, a fertilidade das espiguetas, a massa de 1.000 grãos, a matéria seca da parte aérea, a produtividade e o teor de N na folha bandeira. O sucesso no estabelecimento da cultura do arroz de terras altas no SPD nas regiões de inverno seco do Brasil está diretamente ligado ao mecanismo de distribuição das semeadoras-adubadoras. A haste sulcadora promoveu maior profundidade de deposição de sementes, conseqüentemente reduzindo o estande, o número de panículas por área e a produtividade. A aplicação de N em cobertura no arroz de terras altas em SPD proporciona maiores produtividades quando a semeadura é realizada com mecanismo de disco duplo.

Mecanismos de abertura de sulcos, inoculação e adubação nitrogenada em feijoeiro em sistema plantio direto

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determination of the quality index of a Paleudult under sunflower culture and different management systems

Soil is an essential resource for life and its properties are susceptible to be modified by tillage systems. The impact of management practices on soil functions can be assessed through a soil quality index. It is interesting to assess soil quality in different soil types. Therefore, the aim of this study was to determine the soil quality index of a Paleudult under different management conditions and sunflower culture. The experiment was carried out in Botucatu (SP, Brazil), in an 11-year non-tilled area used for growing soybean and maize during summer and black oat or triticale in winter. Four management systems were considered: no-tillage with a hoe planter (NTh), no-tillage with a double-disk planter (NTd), reduced tillage (RT) and conventional tillage (CT). Soil samples were taken from the planting lines at harvest time. To determine the soil quality indices, following the methodology proposed by Karlen and Stott (1994), three main soil functions were assessed: soil capacity for root development, water storage capacity of the soil and nutrient supply capacity of the soil. The studied Paleudult was considered a soil with good quality under all the observed management systems. However, the soil quality indices varied between treatments being 0.64, 0.68, 0.86 and 0.79 under NTh, NTd, RT and CT, respectively. Physical attributes such as resistance to penetration and macroporosity increased the soil quality index in RT and CT compared to NTh and NTd. The soil quality indices obtained suggested that the evaluated soil is adequate for sunflower production under our study conditions. In view of the SQI values, RT is the most suitable management for this site since it preserves soil quality and provides an acceptable sunflower yield. © 2011 Elsevier B.V.

Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção de beta-lactamase de espectro estendido em membros da família Enterobateriaceae

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Mecanismos sulcadores de semeadora-adubadora na cultura do milho (Zea mays L.) no sistema de plantio direto

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Detecção de genes de beta-lactamases de amplo espectro em Pseudomonas aeruginosa resistentes aos carbapenêmicos isoladas em São José do Rio Preto - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de sistemas de manejo em Nitossolo Vermelho distroférrico e na cultura do girassol

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mecanismos de distribuição do fertilizante e adubação nitrogenada em cobertura no arroz de terras altas em plantio direto

Pós-graduação em Agronomia (Energia na Agricultura) - FCA