DETERMINATION OF ENERGY VALUE OF HAMBURGERS AND MEATBALLS BY DIRECT CALORIMETRY AND FOOD ANALYSIS COMPOSITION. COMPARISON WITH NUTRITION INFORMATION DISPLAYED ON PACKAGES

The nutritional composition found in the laboratory and those present on labels of manufactured foods can differ significantly. The purpose of this study was to determine the nutritional composition of hamburgers and meatballs and compare them with your labels. The food analysis was performed following the Analytical Standards Institute`s Adolfo Lutz and energy content was determined by bomb calorimetry. Regarding the energy value, all the samples had values less than informed on the label. The content of lipids of hamburgers and meatballs ( except the beef) were lower than those reported on the label. The values of protein for the meatballs and chicken hamburger had lower values than those labels. Thus, the labels may overestimate as underestimate some nutritional values, leading to population erroneous information.

Evidence for direct squalene and 2,3-oxidosqualene binding by supernatant protein factor

We present the crystal structures of the SEC14-like domain of supernatant protein factor (SPF) in complex with squalene and 2,3-oxidosqualene. The structures were resolved at 1.75 Å (complex with squalene) and 1.6 Å resolution (complex with 2,3-oxidosqualene), leading in both cases to clear images of the protein/ substrate interactions. Ligand binding is facilitated by removal of the Golgi-dynamics (GOLD) C-terminal domain of SPF, which, as shown in previous structures of the apo-protein, blocked the opening of the binding pocket to the exterior. Both substrates bind into a large hydrophobic cavity, typical of such lipid-transporter family. Our structures report no specific recognition mode for the epoxide group. In fact, for both molecules, ligand affinity is dominated by hydrophobic interactions, and independent investigations by computational models or differential scanning micro-calorimetry reveal similar binding affinities for both ligands. Our findings elucidate the molecular bases of the role of SPF in sterol endo-synthesis, supporting the original hypothesis that SPF is a facilitator of substrate flow within the sterol synthetic pathway. Moreover, our results suggest that the GOLD domain acts as a regulator, as its conformational displacement must occur to favor ligand binding and release during the different synthetic steps.

Dual transcriptional control by Ear3/COUP: negative regulation through the DR1 direct repeat and positive regulation through a sequence downstream of the transcriptional start site of the mouse mammary tumor virus promoter.

Ear3/COUP is an orphan member of the steroid/thyroid hormone receptor superfamily of transcription factors and binds most tightly to a direct repeat of AGGTCA with 1 nucleotide in between (DR1). Ear3/COUP also binds with a similar affinity to the palindromic thyroid hormone response element (TRE). This binding preference of Ear3/COUP is same as that of the retinoid X receptor (RXR), which is another member of the superfamily. In the present study, we identified a sequence responsible for Ear3/COUP-mediated transactivation in the region downstream of the transcription start site of the mouse mammary tumor virus promoter. This cis-acting sequence was unresponsive to RXR. When the DR1 or TRE sequence was added upstream of the promoter, transactivation by Ear3/COUP was completely abolished, whereas RXR enhanced transcription from the promoter. The mode of action of Ear3/COUP could be utilized to control complex gene expressions in morphogenesis, homeostasis, and development.

Parallel direct Poisson and biharmonic solvers /

"NSF - OCA - GJ-36936 - 000006."

Foreign direct investment in the United States : an update : review and analysis of current developments : a report submitted to the Committee on Energy and Commerce, the Committee on Ways and Means, and the Committee on Foreign Affairs of the House of Representatives, to the Committee on Finance, the Committee on Commerce, Science and Transportation, and the Committee on Foreign Relations of the Senate, and to the Joint Economic Committee of the Congress in response to section 3(a) of the Foreign Direct Investment and International Financial Data Improvements Act of 1990.

"January 1995."

Accounting for direct loans and loan guarantees.

"August 23, 1993."

"Miss Lou" : and Driven back to Eden /

Mode of access: Internet.

Practical poultry keeping, for the poultryman, the poultry farmer and the back-yard poultry keeper,

Mode of access: Internet.

The direct isolation and cultivation of human bovine and avian tubercle bacilli /

Thesis (M. S.) - Cornell University, June 1920.

An experimental comparison of direct detailed and directed discovery methods of presenting tape-recorded instruction.

Mode of access: Internet.

The personal narrative of James O. Pattie, of Kentucky : during an expedition from St. Louis, through the vast regions between that place and the Pacific ocean, and thence back through the city of Mexico to Vera Cruz, during journeyings of six years; in which he and his father, who accompanied him, suffered unheard of hardships and dangers, had various conflicts with the Indians, and were made captives, in which captivity his father died; together with a description of the country, and the various nations through which they passed /

Reprint of 1831 Cincinnati ed. and includes facsimile of original t.-p.

Operator's, organizational, direct support, and general support maintenance manual for test set, STE-M1/FVS (4910-01-112-9655).

"January 1985."

Operator, organizational, direct support, and general support maintenance manual : printing press, offset, web-fed, motor driven, 208V, 60 hertz, Hess and Barker model 3CX, FSN 3610-617-7566.

Includes index.

Unit, direct support, and general support maintenance repair parts and special tools lists ... : intrenching machine, combat, high speed mounted, ladder type, NSN 3805-00-727 6719, Unit Rig model 4262.

"June 1992."

Operator, organizational, direct support, and general support maintenance manual : receiving set, radio, AN/URR-69.

"June 1974."