tkCybernetics

tkCybernetics is a simulation software that can be used to create and evaluate simple cybernetic circuits as a combination of lowpass and highpass filters and several nonlinear elements. tkCybernetics has been developed for and successfully applied to introductory courses on biological cybernetics at Bielefeld University. Therefore, it is recommended for students who start learning about cybernetics. Students can quickly grasp the concepts used in the graphical user interface and are able to create their own circuits after a short introduction to the program. Its usefulness for advanced students and researchers is limited to quickly testing circuits. This article provides detailed tutorial information on how to install and work with tkCybernetics for self-learning and for higher education.

Neural Networks as Cybernetic Systems

This book on biological cybernetics combines system theory and artificial neural networks. Following a 'holistic' approach, the book examines the role of simulation in biology. Mainly addressed to students of Biology, the book tries to avoid the use of mathematical formula as far as possible. Exercises can be performed with a related software tool (tkCybernetics) for part 1 as well as with educational simulation on neural networks for part two (in prepration.)

How Minds Work: A Cognitive Theory of Everything - a Tutorial on the IDA Model of Cognition

The IDA model of cognition is a fully integrated artificial cognitive system reaching across the full spectrum of cognition, from low-level perception/action to high-level reasoning. Extensively based on empirical data, it accurately reflects the full range of cognitive processes found in natural cognitive systems. As a source of plausible explanations for very many cognitive processes, the IDA model provides an ideal tool to think with about how minds work. This online tutorial offers a reasonably full account of the IDA conceptual model, including background material. It also provides a high-level account of the underlying computational “mechanisms of mind” that constitute the IDA computational model.

“Digital Diagnostics and Communication (DDC) system” Redesigning the Emergency Ambulance

During the development of a new treatment space for the UK emergency ambulance participatory observations with front-line clinicians revealed the need for an integrated patient monitoring, communication and navigation system. The research identified the different information touch-points and requirements through modes of use analysis, day-in-the-life study and simulation workshops with clinicians. Emergency scenario and role-play with paramedics identified 5 distinct ambulance modes of use. Information flow diagrams were created and checked by paramedics and digital User Interface (UI) wireframes were developed and evaluated by clinicians during clinical evaluations. Feedback from clinicians defined UI design specification further leading to a final design proposal. This research was a further development from the 2007 EPSRC funded “Smart Pods” project. The resulting interactive prototype was co-designed in collaboration with ambulance crews and provides a vision of what could be achieved by integrating well-proven IT technologies and protocols into a package relevant in the emergency medicine field. The system has been reviewed by over 40 ambulance crews and is part of a newly co-designed ambulance treatment space.

Androgen-regulated genes differentially modulated by the androgen receptor coactivator L-dopa decarboxylase in human prostate cancer cells

Background The androgen receptor is a ligand-induced transcriptional factor, which plays an important role in normal development of the prostate as well as in the progression of prostate cancer to a hormone refractory state. We previously reported the identification of a novel AR coactivator protein, L-dopa decarboxylase (DDC), which can act at the cytoplasmic level to enhance AR activity. We have also shown that DDC is a neuroendocrine (NE) marker of prostate cancer and that its expression is increased after hormone-ablation therapy and progression to androgen independence. In the present study, we generated tetracycline-inducible LNCaP-DDC prostate cancer stable cells to identify DDC downstream target genes by oligonucleotide microarray analysis. Results Comparison of induced DDC overexpressing cells versus non-induced control cell lines revealed a number of changes in the expression of androgen-regulated transcripts encoding proteins with a variety of molecular functions, including signal transduction, binding and catalytic activities. There were a total of 35 differentially expressed genes, 25 up-regulated and 10 down-regulated, in the DDC overexpressing cell line. In particular, we found a well-known androgen induced gene, TMEPAI, which wasup-regulated in DDC overexpressing cells, supporting its known co-activation function. In addition, DDC also further augmented the transcriptional repression function of AR for a subset of androgen-repressed genes. Changes in cellular gene transcription detected by microarray analysis were confirmed for selected genes by quantitative real-time RT-PCR. Conclusion Taken together, our results provide evidence for linking DDC action with AR signaling, which may be important for orchestrating molecular changes responsible for prostate cancer progression.

Analysis of human immunodeficiency virus type 1 drug resistance in children receiving nucleoside analogue reverse-transcriptase inhibitors plus nevirapine, nelfinavir, or ritonavir (Pediatric AIDS Clinical Trials Group 377)

In Pediatric AIDS Clinical Trials Group 377, antiretroviral therapy-experienced children were randomized to 4 treatment arms that included different combinations of stavudine, lamivudine (3TC), nevirapine (Nvp), nelfinavir (Nfv), and ritonavir (Rtv). Previous treatment with zidovudine (Zdv), didanosine (ddI), or zalcitabine (ddC) was acceptable. Drug resistance ((R)) mutations were assessed before study treatment (baseline) and at virologic failure. Zdv(R), ddI(R), and ddC(R) mutations were detected frequently at baseline but were not associated with virologic failure. Children with drug resistance mutations at baseline had greater reductions in virus load over time than did children who did not. Nvp(R) and 3TC(R) mutations were detected frequently at virologic failure, and Nvp(R) mutations were more common among children receiving 3-drug versus 4-drug Nvp-containing regimens. Children who were maintained on their study regimen after virologic failure accumulated additional Nvp(R) and 3TC(R) mutations plus Rtv(R) and Nfv(R) mutations. However, Rtv(R) and Nfv(R) mutations were detected at unexpectedly low rates.

Biochemical effects of 3,5-diethoxycarbonyl-1, 4-dihydrocollidine in mouse liver

3,5-Diethoxycarbonyl-1,4-dihydrocollidine (DDC) is a porphyrinogenic agent and is a powerful inducer of δ-aminolaevulinate synthetase, the first and rate-limiting enzyme of the haem-biosynthetic pathway, in mouse liver. However, DDC strikingly inhibits mitochondrial as well as microsomal haem synthesis by depressing the activity of ferrochelatase in vivo. The drug on repeated administration to female mice has been found to elicit hypertrophic effects in the liver microsomes initially, but the effects observed at later stages denote either hyperplasia or increase in polyploidal cells. The microsomal protein concentration shows a striking decrease with repeated doses of the drug. The rate of microsomal protein synthesis in vivo as well as in vitro shows an increase with two injections of DDC but decreases considerably with repeated administration of the drug. The activities of NADPH-cytochrome creductase and ribonuclease are not affected in the liver microsomes of drug-treated animals when expressed per mg of microsomal protein. DDC has also been found to cause degradation of microsomal haem, which is primarily responsible for the decrease in cytochrome P-450 content. The drug also leads to a decrease in mitochondrial cytochrome c levels due to inhibition of haem synthesis and also due to degradation of mitochondrial haem at later stages. The biochemical effects of the drug are compared and discussed with those reported for allylisopropylacetamide and phenobarbital.

Porphyrin synthesis in drug induced hepatic porphyria

Liver δ-aminolaevulate (ALA) synthetase and ALA dehydratase are induced to a greater extent in 3,5-diethoxy carbonyl-1,4-dihydrocollidine (DDC) injected mice as compared to the allyl isopropyl acetamide (AIA) injected rats. DDC treated mice do not show an increase in porphobilinogen (PEG) levels commensurate with the increase in ALA levels and the two enzyme activities, but accumulate enormous quantities of protoporphyrin in the liver. Normal mouse liver has an inherent greater capacity to convert PBG to porphyrins as compared to that of the rat. This together with the inhibition of iron incorporation into protoporphyrin in vivo at later stages of DDC administration can account for the large accumulation of protoporphyrin in these animals.

Studies on induction of delta-aminolevulinic acid synthetase in mouse liver

Administration of 3,5-diethoxy carbonyl-1,4-dihydrocollidine (DDC) to mice resulted in a striking increase in the level of δ-aminolevulinic acid (ALA) synthetase in liver. Although the enzyme activity was primarily localized in mitochondria and postmicrosomal supernatant fluid, a significant level of activity was also detected in purified nuclei. The time course of induction showed a close parallelism between the bound and free enzyme activities with the former always accounting for a higher percentage of the total activity as compared to the latter. Studies with cycloheximide indicated a half-life of around 3 hr for both the bound and free ALA synthetase. Actinomycin D and hemin prevented enzyme induction when administered along with DDC, but when administered 12 hr after DDC treatment Actinomycin D did not lead to a decay of either the bound or free enzyme activity and hemin inhibited the bound enzyme activity but not the free enzyme level. The molecular sizes of the mitochondrial and cytosolic ALA synthetase(s) were found to be similar on sephadex columns.

Synthesis of novel beta-aryl-beta-(methylthio)acroleins via Vilsmeier-Haack protocol as potential 1,3-dielectrophilic three-carbon building blocks

A new general route for the synthesis of novel beta-aryl-beta-(methylthio)acroleins, a class of stable potential 1,3-dielectrophilic synthons, has been reported. The overall protocol involves treatment of either beta-chloroacroleins or their precursor iminium salts (generated in situ from the corresponding active methylene ketones under Vilsmeier-Haack reaction conditions) with S,S-dimethyldithiocarbonates (DDC)/aqueous KOH in either a one-pot or two-step process. The dimethyldithiocarbonate (DDC)/30% aqueous KOH has been shown to be an excellent source of methylthiolate anion. (C) 2014 Elsevier Ltd. All rights reserved.

Comportamiento productivo de cerdos alimentados con desperdicios de cocina y residuos de galleta en la dieta, bajo distintos porcentajes de inclusión

El presente estudio se realizó en la granja porcina de la Universidad Nacional Agraria (U.N.A), ubicada en el Km. 13 a los 86°. 09' 36" longitud oeste y los 12° 08'15" latitud norte de la comunidad de Sabana Grande, Municipio de Managua con un elevación de 56 m sobre el nivel del mar (INETER, 1987). La fase de campo fue realizada de Julio a Octubre de 1999, con el objetivo de evaluar el comportamiento productivo de cerdos en crecimiento, desarrollo y engorde alimentados con tres raciones diferentes: (T1) Desperdicio de Galleta 100%, (T2) Desperdicio de Galleta 75% + Desperdicio de cocina 25%, (T3) Desperdicio de cocina 100%. Se utilizaron 18 cerdos comerciales de ambos sexos, con peso promedio de inicio de 54.86 kg, que fueron distribuidos en tres tratamientos con seis repeticiones con los datos levantados de pesaje de los diferentes tratamientos, se efectúo el análisis estadístico correspondiente al diseño experimental completamente al Azar (DCA) y su correspondiente análisis para las variables en estudio, G.M D. y C.A. Los cerdos que presentaron una ganancia de peso más alta, fueron los que se alimentaron con la ración l00 % de desperdicio de cocina teniendo una ganancia media diaria de 0.758 kg. los cerdos alimentados 75 % DDG, + 25 % DDC, alcanzaron una ganancia de peso promedio de 0.578 kg. mientras que el tratamiento 100% DDG alcanzo una ganancia media de peso promedio de 0.17 kg. El análisis de la ganancia media diaria se demostró con una alfa de 5 %. El análisis estadístico mostró que las diferencias en conversión alimenticia fueron significativas entre tratamientos con un Alfa al 5 %. Al comparar las utilidades generadas entre el T t y T2, estos resultaron financieramente aceptables, de forma similar se comportaron el T1 y T3., no obstante, desde el punto de vista nutricional, estos no cumplieron los requerimientos de la categoría, por lo que el T2, se presentó finalmente como el más aceptable nutricionalmente.

A documentação de Paul Otlet : uma proposta para a organização racional da produção intelectual do homem

A pesquisa procura identificar e analisar as circunstâncias e motivações teóricas que levaram ao surgimento, no final do século XIX, da área de estudo denominada Documentação. Apresenta informações biográficas sobre os principais artífices deste projeto, os advogados e bibliógrafos belgas, Paul Otlet e Henri La Fontaine, que visaram ampliar a compreensão sobre o meio social e cultural em que atuaram. Procura ampliar a discussão sobre os fatores que motivaram em 1895 a proposta de organização racional de toda a produção intelectual do homem. Apresenta em linhas gerais a visão abrangente e integradora da Documentação que eliminando barreiras físicas, acessava acervos arquivísticos, bibliográficos e museológicos para o registro integral dos assuntos pesquisados. Discute a utilização da Classificação Decimal de Dewey (CDD) na criação do Repertório Bibliográfico Universal e analisa o processo que levou ao surgimento da Classificação Decimal Universal (CDU). Conclui sugerindo a inclusão desta visão extensiva e integradora da documentação ao referencial teórico da Ciência da Informação. Sugere que o resgate deste aporte teórico poderá contribuir para um melhor enfrentamento dos problemas da gestão do conhecimento registrado, produzido e acumulado até os dias atuais, nos mais diversos formatos, suportes e repositórios.