Kokoonpanon kehittäminen tuulivoimalakoneikkojen valmistuksessa

Tämä diplomityö on tehty Hollming Works Oy:n Loviisan yksikölle. Työn tekovaiheessa yrityksessä oltiin aloittamassa tuulivoimalakoneikkojen sarjatyönä tapahtuvaa kokoonpanoa. Työn tavoitteena oli kehittää koneikkojen kokoonpanoverstaan toimintaa. Ennen työn alkua yritykseen oli perustettu tuulivoimalakoneikkojen kokoonpanoon tuoteverstas. Verstaalla kootaan raskaita osakokoonpanoja, jotka lopulta yhdistetään koneikoksi, jota kutsutaan myös naselliksi. Tämän jälkeen varusteluvaiheessa koneikkoon asennetaan mm. erilaisia sähköisiä ja hydraulisia järjestelmiä. Varusteluvaiheen päätteeksi koneikon toiminta testataan. Viimeisenä vaiheenakoneikon päälle asennetaan lasikuitukuori. Työn alkuosassa on käyty läpi tuotannon- ja materiaalinohjauksen perusteita, kokoonpanon kehittämistä ja layout-suunnittelua, jonka pohjalta tuulivoimalaverstaalle tehtiin kokoonpanosoluihin perustuva layoutsuunnitelma. Tuotannonohjaus suunnitelmassa perustuu kapeikko-ohjaukseen. Tuotannon kapeikon muodostava varusteluvaihe imee aiemmista vaiheista osakokoonpanot. Varusteluvaiheen ja testauksen jälkeen koneikko siirtyytyöntöohjatusti lasikuitukuoren asennukseen. Järjestelmässä pyritään tehokkaaseen tilankäyttöön, lyhyeen läpäisyaikaan ja vähäiseen keskeneräisen tuotannon määrään.

Guerra e o desenvolvimento científico: uma superaula interdisciplinar e investigativa construída em torno de um experimento central

This research presents the description of the assembly, development and execution of an experiment and of an interdisciplinary and student research activity built by some undergraduates from Biological Sciences, Physics, Mathematics and Chemistry PIBID subprojects of the Faculty of Science, FC - UNESP, Bauru/SP Campus. These subprojects are part of a single interdisciplinary group, which has as its main activity the Superclass, which consists of an intervention of the PIBID members during the class schedule, with an interdisciplinary and student research didactic sequence, applied by four groups of ten members, in two local State Schools. The experiment treated in this assignment was a miniature cannon (minicannon), and it was used in a Superclass that had the War and Science Development as its theme, wherein the knowledge of all areas were built from the analysis of the multiple variables involved on the cannon shots. These variables were surveyed with the students through the experiment research, during the Superclass, being focused on three of them: the shot and the explosives; the trajectory; and the target. The experiment was extremely successful, because it could instigate the students, who were interested, questioning and debaters for several times during the class. The minicannon made possible the interdisciplinary and the student research work among the four areas of science involved. Despite it has been a laborious experiment in its construction, it has proven to be innovative, and its use has transformed the students‟ ingenuous curiosity into epistemological curiosity, a key factor in the construction of scientific knowledge, wherein the dialogue established in the Superclass explored this new curiosity

De Novo Assembly And Transcriptome Analysis Of The Rubber Tree (hevea Brasiliensis) And Snp Markers Development For Rubber Biosynthesis Pathways.

Hevea brasiliensis (Willd. Ex Adr. Juss.) Muell.-Arg. is the primary source of natural rubber that is native to the Amazon rainforest. The singular properties of natural rubber make it superior to and competitive with synthetic rubber for use in several applications. Here, we performed RNA sequencing (RNA-seq) of H. brasiliensis bark on the Illumina GAIIx platform, which generated 179,326,804 raw reads on the Illumina GAIIx platform. A total of 50,384 contigs that were over 400 bp in size were obtained and subjected to further analyses. A similarity search against the non-redundant (nr) protein database returned 32,018 (63%) positive BLASTx hits. The transcriptome analysis was annotated using the clusters of orthologous groups (COG), gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Pfam databases. A search for putative molecular marker was performed to identify simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). In total, 17,927 SSRs and 404,114 SNPs were detected. Finally, we selected sequences that were identified as belonging to the mevalonate (MVA) and 2-C-methyl-D-erythritol 4-phosphate (MEP) pathways, which are involved in rubber biosynthesis, to validate the SNP markers. A total of 78 SNPs were validated in 36 genotypes of H. brasiliensis. This new dataset represents a powerful information source for rubber tree bark genes and will be an important tool for the development of microsatellites and SNP markers for use in future genetic analyses such as genetic linkage mapping, quantitative trait loci identification, investigations of linkage disequilibrium and marker-assisted selection.

The development and application of a novel safety-catch linker for BOC-based assembly of libraries of cyclic peptides

Cyclic peptides are appealing targets in the drug-discovery process. Unfortunately, there currently exist no robust solid-phase strategies that allow the synthesis of large arrays of discrete cyclic peptides. Existing strategies are complicated, when synthesizing large libraries, by the extensive workup that is required to extract the cyclic product from the deprotection/cleavage mixture. To overcome this, we have developed a new safety-catch linker. The safety-catch concept described here involves the use of a protected catechol derivative in which one of the hydroxyls is masked with a benzyl group during peptide synthesis, thus making the linker deactivated to aminolysis. This masked derivative of the linker allows BOC solid-phase peptide assembly of the linear precursor. Prior to cyclization, the linker is activated and the linear peptide deprotected using conditions commonly employed (TFMSA), resulting in deprotected peptide attached to the activated form of the linker. Scavengers and deprotection adducts are removed by simple washing and filtration. Upon neutralization of the N-terminal amine, cyclization with concomitant cleavage from the resin yields the cyclic peptide in DMF solution. Workup is simple solvent removal. To exemplify this strategy, several cyclic peptides were synthesized targeted toward the somatostatin and integrin receptors. From this initial study and to show the strength of this method, we were able to synthesize a cyclic-peptide library containing over 400 members. This linker technology provides a new solid-phase avenue to access large arrays of cyclic peptides.

Lista de las principales resoluciones relacionadas con la Estrategia Internacional del Desarrollo y el Programa de Acción Regional aprobadas por la Asamblea General y el Consejo Económico y Social de las NU, la CEPAL y el CDCC = List of main resolutions adopted with regard to the International Development Strategy and the Regional Programme of Action by the General Assembly, United Nations Economic and Social Council, ECLA and CDCC

Contiene el título de las resoluciones y su número.

Recopilación de resoluciones relacionadas con la Estrategia Internacional del Desarrollo y el Nuevo Orden Económico Internacional aprobadas por la Asamblea General y el Consejo Económico y Social de las NU, CEPAL y CDCC = Collected resolutions on the International Development Strategy and the New International Economic Order adopted by the United Nations General Assembly and Economic and Social Council, the CEPAL and the CDCC

Incluye Bibliografía

Estrategia Internacional del Desarrollo: programa de acción de la Asamblea General para el Segundo Decenio de las Naciones Unidas para el Desarrollo = International Development Strategy: action programme of the General Assembly for the Second United Nations Development Decade

Incluye Bibliografía

Algunas reflexiones acerca del período extraordinario de sesiones de la Asamblea General y de la Estrategia Internacional del Desarrollo = Some reflections on the special session of the General Assembly and the International Development Strategy

Incluye Bibliografía

Report of the High Level Follow up Meeting to the United Nations General Assembly 60th Session 2005 World Summit: Strengthening Social Development, the Way Forward for Caribbean SIDS

Includes bibliography

Selección de resoluciones aprobadas por la Asamblea General y el Consejo Económico y Social (ECOSOC) durante el período 1981-1988 acerca de la situación de la mujer y su integración en el desarrollo económico y social = A selection of resolutions adopted by the General Assembly and the Economic and Social Council (ECOSOC) between 1981 and 1988 on the situation of women and their integration into economic and social development

Contiene resoluciones aprobadas por la Asamblea General y el ECOSOC durante los años 1981 y 1988 sobre la situación y participación de la mujer en el desarrollo económico y social.