Somatic embryogenesis from zygotic embryos of Euterpe oleracea Mart.

The aim of this work was to study the morphogenetic responses of zygotic embryos of açai palm (Euterpe oleracea Mart.) submitted to several conditions of in vitro culture. Several research experiments were conducted, in laboratory, using vegetable material collected from açai palm plants at Embrapa Amazon Oriental, Belém-PA, Brazil. It was possible to verify the expression of a direct, repetitive and no-synchronized model of somatic embryogenesis in mature zygotic embryos cultivated in primary MS medium supplemented with 2,4-D (339.36 muM) and transferred to a secondary MS medium in the presence of NAA (0.537 muM) and 2iP (12.30 muM). The conversion of somatic embryos into seedlings was reached after 210 days with the transfer of the cultures to a third medium with sucrose and mineral salts concentrations reduced to a half, without growth regulators.

Desenvolvimento do açaizeiro de terra firme, cultivar pará, sob atenuação da radiação solar em fase de viveiro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chemical control of pilea microphylla in euterpe oleraceae nurseries with oxyfluorfen

The açai palm is a Brazilian native species and economically important, grown for the hearts of palm and fruit extraction, as well as the seedling production for ornamental purposes. The weed control is one of the concerns observed in seedling production, which has the artillery plant (Pilea microphylla) with the major and widespread occurrence. Thus, the aim of this trial was to evaluate the selectivity and efficacy of oxyfluorfen in artillery plant control in açai (Euterpe oleracea) seedlings. The experimental design was completely randomized. The treatments were arranged in a 2 x 3 factorial scheme, using 2 types of herbicide application: broadcast spraying (sprayed over total area) and spot spraying (directed to the target) and 3 oxyfluorfen dosages (0, 72 and 144 g of active ingredient ha-1) with four replications. Each experimental unit consisted by one açai palm pot infested by artillery plant. They ere visually evaluated for seedling toxicity and the weed control percentage, at 7, 15, 30, 45 and 60 days after application (DAA). There was 100% control of artillery plant from 15 to 60 DAA for the two tested dosages, regardless of application type. However, 100% control was observed at 7 days, using 1.5 L ha-1 in the spot type. The effects of phytotoxicity from 30 to 60 days was moderate, regardless the application method or dosage.

Avaliação de substratos orgânicos no cultivo de orquídeas nativas da APA ilha do Combu, Belém, Pará, Brasil

As fibras naturais vem se destacando no cultivo de orquídeas, entre elas a fibra do coco é a mais promissora. Entretanto, existem outros resíduos orgânicos naturais que podem usados no cultivo. Foi realizado um estudo na Área de Proteção Ambiental Ilha do Combu, localizada no município de Belém, com o objetivo de avaliar os efeitos de substratos orgânicos no cultivo de orquídeas. As orquídeas selecionadas foram Brassia chloroleuca Barb.Rodr. e Sobralia macrophylla Rchb f. ambas nativas da APA. Entre os substratos testados, incluiu-se produto do aproveitamento do açaí, a palmeira mais freqüente na ilha. As orquídeas foram submetidas a quatro substratos considerados como tratamentos (T1 - fibra do caule do açaizeiro; T2 - fibra do coco; T3 - sementes do açaizeiro e T4 - serragem). O delineamento experimental foi inteiramente casualizado com 4 tratamentos e 4 repetições (10 indivíduos/repetição). Os parâmetros avaliados foram: o diâmetro, o comprimento, o número de brotações, número de folhas e Taxa de Sobrevivência para pseudobulbos de Brassia chloroleuca e para rebrotações de Sobralia macrophylla. Os dados foram inseridos em planilhas do Programa Bioestat 5.0. e submetidos à análise de variância e as médias comparadas pelo teste Tuckey a 5% para avaliar o grau de significância dos efeitos dos tratamentos. Os resultados mostraram que a fibra do caule do açaí promoveu o melhor desenvolvimento das estruturas vegetativas seguida da serragem em pseudobulbos de B. chloroleuca e para S. macrophylla a fibra do caule e a semente do açaí promoveram o maior desenvolvimento vegetativo. Conclui-se que a fibra do caule do açaizeiro pode ser utilizada no cultivo das espécies com aproveitamento sustentável e ecológico após o corte do palmito do açaizeiro.

História, memória e natureza: as comunidades da Ilha do Combu-Belém-PA

A utilização dos recursos naturais e do território perpassa as formas econômicas, sociais e simbólicas de produção e reprodução das populações ditas tradicionais. No caso da comunidade da Ilha do Combu, as estratégias de reprodução e utilização dos recursos naturais e do território inferem entre aqueles aspectos, ora através do manejo e extrativismo do açaí, que mantém 90% da renda no período de safra, ora na utilização, seleção a apropriação da memória como forma de afirmação de valores e atitudes frente à disputa pelo território, relacionando-se com a reprodução da própria comunidade enquanto sujeitos ativos, nas disputas hegemônicas de poder na atualidade.

Regeneração, manejo e exploração de açaizais nativos de várzea do estuário amazônico

O açaizeiro (Euterpe oleracea Mart.) é a maior fonte supridora de matéria-prima para a indústria de palmito no Brasil. A forma com que a espécie vem sendo explorada tem ocasionado, em algumas áreas, a sua degradação e a redução da oferta de frutos. O objetivo deste trabalho foi o de estudar o processo de regeneração natural dos açaizais nativos de várzea do estuário amazônico que foram alterados em decorrência da extração do palmito. A pesquisa foi conduzida no município de Igarapé-Miri, Pará, Brasil, onde foram analisados fatores fenológicos vegetativos, de crescimento e socioeconômicos, visando subsidiar a definição de técnicas que auxiliem o manejo racional do açaizeiro. Para a realização dos estudos foram selecionadas quatro áreas de açaizais com diferentes períodos após a extração do palmito (0, 12, 24 e 36 meses) para a obtenção de dados sobre a população de plantas, emissão de perfilhos, altura, diâmetro e número de folhas dos estipes, produção de matéria seca, produtividade e rentabilidade dos açaizais manejados e não-manejados. Os resultados mostram que aos 48 meses após a extração do palmito os açaizais nativos encontram-se reabilitados e voltam a ser produtivos, apresentando uma população de 1.600 plantas/ha, das quais 30 % iniciaram a produção de frutos e 50 % encontravam-se aptas a produzir palmito. Verificou-se que a coleta de frutos é mais lucrativa que a extração de palmito, e que o manejo é uma prática que aumenta a produtividade e a rentabilidade dos açaizais nativos de várzea.

Unusual branching in the palm Euterpe edulis Mart

The unusual development of branches along the stem of Euterpe edulis is described for the first time. Branches originated at 2 to 190 cm from the ground. Ramified individuals and branches were able to produce reproductive structures and some branches produced roots. A plausible cause for the observed anomaly could be genetic problems due to small population sizes. The better agreement of this process can have a positive effect in the harvest of the heart of palm through the artificial induction of sprouts, what would prevent the death of the individual.

Physiological and biochemical characterization of the assai palm (Euterpe oleracea Mart.) during seed germination and seedling growth under aerobic and anaerobic conditions

Physiological and biochemical aspects of assai palm during seed germination and early seedling growth were investigated. Seeds collected from plants growing in flooded and upland forests were used to determine the influence of normoxic (aerobic) and anoxic (anaerobic) conditions in germination and the initial and average time of development in the roots and shoots. After 75 days, seedlings germinated under normoxia were transferred to trays and submitted to flooding. Seed reserves (lipids, proteins, soluble sugars and starch) were monitored for quiescent and germinated seeds maintained under normoxic and anoxic conditions, as well as after 5, 10 and 20 days of seedling growth. Alcohol dehydrogenase (ADH) activity was quantified in roots and leaves of seedlings without or with flooding (partial and total). Seeds were not able to germinate under anoxia. Different strategies of storage mobilization of lipids, proteins, soluble sugars and starch were observed in seeds of each environment. ADH activity was induced by anoxia, with the highest level observed in the leaves. This study showed that, under normoxic conditions, the best developmental performance of assai palm seeds, from flooded or upland forest areas, during germination was associated with primary metabolites mobilization and seedling flooding tolerance with increased ADH activity. We conclude that the assai palm is well adapted to the anoxic conditions provoked by flooding.

Fractionation and interesterification of palm (Elaeis guineensis) oil from Peruvian Amazon

In the present work, the physical and chemical characteristics of the fruit of the oily palm coming from the river basin of the Manitf (Region Loreto - Peru) were studied. Also, the fractionation of the palm oil and the interesterification of mixtures of palm oil/estearin was carried out. Physico- chemical properties of the crude oil and of the products obtained and fatty acids were analysed by gas chromatography The level of saturated fatty acids increased from 51,17% in the palm oil to 54,31% in the stearin. The best products for the food industry were the interesterified samples as they had melting points close to 37 degrees C.

Influence of palm fatty acid distillate on rumen degradability and protozoa population in buffaloes

Oil and fat as energy sources at low cost are relevant in ruminant nutrition. The aim of this study was to evaluate the effects of palm fatty acid distillate (PFAD) on the degradability and ciliate protozoa population in buffalo. Four rumen fistulated buffaloes were fed a basal diet in a Latin square (4x4) design trial. Treatments were designed with four of different levels of PFAD added directly into the rumen: 0; 200; 420 and 500 g/animal/d. High levels of PFAD (420 and 500 g/d) promoted higher degradation of the soluble fraction and lower in potentially degradable fraction of dry matter (DM) and neutral detergent fibre (NDF) with lower values of potential and effective degradability in two evaluated grasses, bermudagrass and brachiariagrass. Significant decreases in the total number of protozoa/mL of rumen content, Entodinium and ciliates belonging to subfamily Diplodiniinae were observed at higher level of PFDA addition in the rumen. Also, Epidinium and Holotrich ciliates disappeared from the rumen. Significant correlations were observed of the ciliate concentration and composition as a function of dietary lipids content. Entodinium composition increased from 68.0% to 99.6% and Diplodiniinae reduced from 30.4% to 0.4% with increasing PFAD level indicating higher fat toxicity effect on the Diplodiniinae ciliates than Entodinium species and direct action of the larger ciliates on the fibre degradation.

Performance of different immobilized lipases in palm oil biodiesel synthesis

Performance of different immobilized lipases in palm oil biodiesel synthesis. Optimized conditions for palm oil and ethanol enzymatic biodiesel synthesis were determined with different immobilized lipases SiO(2)-PVA-immobilized lipase from Pseudomonas fluorescens and acrylic resin-immobilized lipase, Novozym (R) 435, from Candida antartica, in solvent-free medium. A full factorial design assessed the influence of temperature (42 - 58 degrees C) and ethanol: palm oil (6:1 - 18:1) molar ratio on the transesterification yield. Main effects were adjusted by multiple regression analysis to linear models and the maximum transesterification yield was obtained at 42 degrees C and 18:1 ethanol: palm oil molar ratio. Mathematical models featuring total yield for each immobilized lipase were suitable to describe the experimental results.

Greenhouse gas emissions and energy balance of palm oil biofuel

The search for alternatives to fossil fuels is boosting interest in biodiesel production. Among the crops used to produce biodiesel, palm trees stand out due to their high productivity and positive energy balance. This work assesses life cycle emissions and the energy balance of biodiesel production from palm oil in Brazil. The results are compared through a meta-analysis to previous published studies: Wood and Corley (1991) [Wood BJ, Corley RH. The energy balance of oil palm cultivation. In: PORIM intl. palm oil conference agriculture; 1991.], Malaysia; Yusoff and Hansen (2005) [Yusoff S. Hansen SB. Feasibility study of performing an life cycle assessment on crude palm oil production in Malaysia. International Journal of Life Cycle Assessment 2007;12:50-8], Malaysia; Angarita et al. (2009) [Angarita EE, Lora EE, Costa RE, Torres EA. The energy balance in the palm oil-derived methyl ester (PME) life cycle for the cases in Brazil and Colombia. Renewable Energy 2009;34:2905-13], Colombia; Pleanjai and Gheewala (2009) [Pleanjai S. Gheewala SH. Full chain energy analysis of biodiesel production from palm oil in Thailand. Applied Energy 2009;86:S209-14], Thailand; and Yee et al. (2009) [Yee KF, Tan KT, Abdullah AZ, Lee la. Life cycle assessment of palm biodiesel: revealing facts and benefits for sustainability. Applied Energy 2009;86:S189-96], Malaysia. In our study, data for the agricultural phase, transport, and energy content of the products and co-products were obtained from previous assessments done in Brazil. The energy intensities and greenhouse gas emission factors were obtained from the Simapro 7.1.8. software and other authors. These factors were applied to the inputs and outputs listed in the selected studies to render them comparable. The energy balance for our study was 1:5.37. In comparison the range for the other studies is between 1:3.40 and 1:7.78. Life cycle emissions determined in our assessment resulted in 1437 kg CO(2)e/ha, while our analysis based on the information provided by other authors resulted in 2406 kg CO(2)e/ha, on average. The Angarita et al. (2009) [Angarita EE, Lora EE, Costa RE, Torres EA. The energy balance in the palm oil-derived methyl ester (PME) life cycle for the cases in Brazil and Colombia. Renewable Energy 2009:34:2905-13] study does not report emissions. When compared to diesel on a energy basis, avoided emissions due to the use of biodiesel account for 80 g CO(2)e/MJ. Thus, avoided life Cycle emissions associated with the use of biodiesel yield a net reduction of greenhouse gas emissions. We also assessed the carbon balance between a palm tree plantation, including displaced emissions from diesel, and a natural ecosystem. Considering the carbon balance outcome plus life cycle emissions the payback time for a tropical forest is 39 years. The result published by Gibbs et al. (2008) [Gibbs HK, Johnston M, Foley JA, Holloway T, Monfreda C, Ramankutty N, et al., Carbon payback times for crop-based biofuel expansion in the tropics: the effects of changing yield and technology. Environmental Research Letters 2008;3:10], which ignores life cycle emissions, determined a payback range for biodiesel production between 30 and 120 years. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.

A new flat mite (Acari: Prostigmata: Tenuipalpidae) from date palm in the Sultanate of Oman

Tenuipalpus omani n. sp. (Acari: Tenuipalpidae) is described from the Sultanate of Oman, based on adults of both sexes collected on date palm, Phoenix dactylifera L. (Arecaceae). This new species is most similar to T. eriophyoides Baker, T. pareriophyoides Meyer & Gerson and T. yarensis Hasan, Bashir & Wakil, also collected from date palm.

Exploration of the acarine fauna on coconut palm in Brazil with emphasis on Aceria guerreronis (Acari : Eriophyidae) and its natural enemies

Coconut is an important crop in tropical and subtropical regions. Among the mites that infest coconut palms, Aceria guerreronis Keifer is economically the most important. We conducted surveys throughout the coconut growing areas of Brazil. Samples were taken from attached coconuts, leaflets, fallen coconuts and inflorescences of coconut palms in 112 localities aiming to determine the Occurrence and the distribution of phytophagous mites, particularly A. guerreronis, and associated natural enemies. Aceria guerreronis was the most abundant phytophagous mite followed by Steneotarsonemus concavuscutum Lofego & Gondim Jr. and Steneotarsonemus furcatus De Leon (Tarsonemidae). Infestation by A. guerreronis was recorded in 87% of the visited localities. About 81% of all predatory mites belonged to the family Phytoseiidae, mainly represented by Neoseiulus paspalivorus De Leon, Neoseiulus baraki Athias-Henriot and Amblyseius largoensis Muma; 12% were Ascidae, mainly Proctolaelaps bickleyi Bram, Proctolaelaps sp nov and Lasioseius subterraneus Chant. Neoseiulus paspalivorus and N. baraki were the most abundant predators on attached coconuts. Ascidae were predominant on fallen coconuts, while A. largoensis was predominant on leaflets; no mites were found on branches of inflorescences. Leaflets harboured higher mite diversity than the attached coconuts. Mite diversity was the highest in the state Para and on palms surrounded by seasonal forests and Amazonian rain-forests. Neoseiulus paspalivorus, N. baraki and P. bickleyi were identified as the most promising predators of A. guerreronis. Analyses of the influence of climatic factors revealed that dry ambient conditions favour the establishment of A. guerreronis. Neoseiulus paspalivorus and N. baraki have differing climatic requirements; the former being more abundant in warm and dry areas, the latter prevailing ill moderately tempered and humid areas. We discuss the significance of our findings for natural and biological control of A. guerreronis.

Seed development, yield and quality of two palm species growing in different tropical forest types in SE Brazil: implications for ecological restoration

Natural forest remnants have been set as seed production fields to supply seeds of native tree species for tropical forest restoration, but the effect of different forest types on seed production has not been accessed to date for palm species. In this work, we studied seed development, yield, and quality of two palm species in different tropical forest types in SE Brazil. Seed production of palmiteiro (Euterpe edulis) and queen-palm (Syagrus romanzoffiana), which are largely used in restoration efforts due to their importance for vertebrate frugivores, were studied in natural remnants of Atlantic Rainforest, Restinga Forest, Seasonally Dry Forest, and Cerrado Forest. We studied seed development, yield, size, and germination of seed lots produced in some of these forest types, including seeds harvested in 2008, 2009, and both years. Seed yield and quality, as well as seed dry mass in 2009, were higher for palmiteiro seeds produced in the Atlantic Rainforest, while queen-palm seeds produced at the Restinga Forest showed the higher mass and yield, but the lowest physiological potential. Consequently, these natural differences of seed yield and quality have to be taken into account for establishing standards for seed commercialization and analysis, seed pricing, and seedling production in forest nurseries.