992 resultados para Zoological specimens


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Mode of access: Internet.

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Includes bibliographical references (p. 71-78).

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Mode of access: Internet.

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Collection of zoological specimens presented to the University of Upsala.

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Includes abstract.

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A total of 378 specimens from 25 collecting localities belonging to 31 different species of fish collected mainly from the rivers of the hilly and mountain regions of the south-western and southern Ceylon have been identified and recorded. Ecological data and water analyses of these collecting localities are given.

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In the present study the specimens collected by the Austrian-Ceylones hydrobiological mission 1970 are described. The freshwater gastropods in this study were collected from 100 different localities, mostly running waters, but some were also collected from stagnant waters like pools, irrigated paddy fields, swamps and water reservoirs (tanks). Listed from these localities are 31 species (and subspecies) 28 species are Streptoneura (=Prosobranchia), 3 species are Euthyneura-Pulmonata Basommatophora.

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A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum, parvo biovar, mba types 1, 3, and 6, in cultured clinical specimens. The primer pairs were designed by using the polymorphic base positions within a 310- to 311-bp fragment of the 5* end and upstream control region of the mba gene. The specificity of the assay was confirmed with reference serovars 1, 3, 6, and 14 and by the amplified-fragment sizes (81 bp for mba 1, 262 bp for mba 3, and 193 bp for mba 6). A more sensitive nested PCR was also developed. This involved a first-step PCR, using the primers UMS-125 and UMA226, followed by the nested mba-type PCR described above. This nested PCR enabled the detection and typing of small numbers of U. urealyticum cells, including mixtures, directly in original clinical specimens. By using random amplified polymorphic DNA (RAPD) PCR with seven arbitrary primers, we were also able to differentiate the two biovars of U. urealyticum and to identify 13 RAPD-PCR subtypes. By applying these subtyping techniques to clinical samples collected from pregnant women, we established that (i) U. urealyticum is often a persistent colonizer of the lower genital tract from early midtrimester until the third trimester of pregnancy, (ii) mba type 6 was isolated significantly more often (P 5 0.048) from women who delivered preterm than from women who delivered at term, (iii) no particular ureaplasma subtype(s) was associated with placental infections and/or adverse pregnancy outcomes, and (iv) the ureaplasma subtypes most frequently isolated from women were the same subtypes most often isolated from infected placentas.