Regulation of Radial Glial Motility by Visual Experience

Radial glia in the developing optic tectum express the key guidance molecules responsible for topographic targeting of retinal axons. However, the extent to which the radial glia are themselves influenced by retinal inputs and visual experience remains unknown. Using multiphoton live imaging of radial glia in the optic tectum of intact Xenopus laevis tadpoles in conjunction with manipulations of neural activity and sensory stimuli, radial glia were observed to exhibit spontaneous calcium transients that were modulated by visual stimulation. Structurally, radial glia extended and retracted many filopodial processes within the tectal neuropil over minutes. These processes interacted with retinotectal synapses and their motility was modulated by nitric oxide (NO) signaling downstream of neuronal NMDA receptor (NMDAR) activation and visual stimulation. These findings provide the first in vivo demonstration that radial glia actively respond both structurally and functionally to neural activity, via NMDAR-dependent NO release during the period of retinal axon ingrowth.

Bidirectional, experience-dependent regulation of N-methyl-d-aspartate receptor subunit composition in the rat visual cortex during postnatal development

In the visual cortex, as elsewhere, N-methyl-d-aspartate receptors (NMDARs) play a critical role in triggering long-term, experience-dependent synaptic plasticity. Modifications of NMDAR subunit composition alter receptor function, and could have a large impact on the properties of synaptic plasticity. We have used immunoblot analysis to investigate the effects of age and visual experience on the expression of different NMDAR subunits in synaptoneurosomes prepared from rat visual cortices. NMDARs at birth are comprised of NR2B and NR1 subunits, and, over the first 5 postnatal weeks, there is a progressive inclusion of the NR2A subunit. Dark rearing from birth attenuates the developmental increase in NR2A. Levels of NR2A increase rapidly (in <2 hr) when dark-reared animals are exposed to light, and decrease gradually over the course of 3 to 4 days when animals are deprived of light. These data reveal that NMDAR subunit composition in the visual cortex is remarkably dynamic and bidirectionally regulated by sensory experience. We propose that NMDAR subunit regulation is a mechanism for experience-dependent modulation of synaptic plasticity in the visual cortex, and serves to maintain synaptic strength within an optimal dynamic range.

Visual biology of Hawaiian coral reef fishes. II. Colors of Hawaiian coral reef fish

The colors of 51 species of Hawaiian reef fish have been measured using a spectrometer and therefore can be described in objective terms that are not influenced by the human visual experience. In common with other known reef fish populations, the colors of Hawaiian reef fish occupy spectral positions from 300-800nm; yellow or orange with blue, yellow with black, and black with white are the most frequently combined colors; and there is no link between possession of ultraviolet (UV) reflectance and UV visual sensitivity or the potential for UV visual sensitivity. In contrast to other reef systems, blue, yellow, and orange appear more frequently in Hawaiian reef fish. Based on spectral quality of reflections from fish skin, trends in fish colors can be seen that are indicative of both visually driven selective pressures and chemical or physical constraints on the design of colors. UV-reflecting colors can function as semiprivate communication signals. White or yellow with black form highly contrasting patterns that transmit well through clear water. Labroid fishes display uniquely complex colors but lack the ability to see the UV component that is common in their pigments. Step-shaped spectral curves are usually long-wavelength colors such as yellow or red, and colors with a peak-shaped spectral curves are green, blue, violet, and UV.

Estrabismo, acuidade visual e erro refrativo não corrigido na criança em idade escolar

Preventable visual loss in children is an important public health problem. The critical period of susceptibility to deprivation or abnormal visual experience has been identified since the early 1970s. Preventable visual loss caused by amblyopia (0.3%–4%) and its risk factors such as strabismus (2.1%–4.6%) and uncorrected refractive errors (5%–7.7%) represent an important public health problem. Thus the primary justification for preschool vision screening is the detection of amblyopia or amblyogenic refractive, strabismic, or ocular disease conditions. However in Portugal there has been little investigation regarding prevalence of visual anomalies among school-age children. Data on the prevalence are lacking but are needed for planning vision services. Aims: 1) Determine the prevalence of strabismus; 2) Determine the prevalence of decreased visual acuity; 3) Determine the prevalence of uncorrected refractive error.

Development of projections from auditory to visual areas in the cat.

In newborn kittens, cortical auditory areas (including AI and AII) send transitory projections to ipsi- and contralateral visual areas 17 and 18. These projections originate mainly from neurons in supragranular layers but also from a few in infragranular layers (Innocenti and Clarke: Dev. Brain Res. 14:143-148, '84; Clarke and Innocenti: J. Comp. Neurol. 251:1-22, '86). The postnatal development of these projections was studied with injections of anterograde tracers (wheat germ agglutinin-horseradish peroxidase [WGA-HRP]) in AI and AII and of retrograde tracers (WGA-HRP, fast blue, diamidino yellow, rhodamine-labeled latex beads) in areas 17 and 18. It was found that the projections are nearly completely eliminated in development, this, by the end of the first postnatal month. Until then, most of the transitory axons seem to remain confined to the white matter and the depth of layer VI; a few enter it further but do not appear to form terminal arbors. As for other transitory cortical projections the disappearance of the transitory axons seems not to involve death of their neurons of origin. In kittens older than 1 month and in normal adult cats, retrograde tracer injections restricted to, or including, areas 17 and 18 label only a few neurons in areas AI and AII. Unlike the situation in the kitten, nearly all of these are restricted to layers V and VI. A similar distribution of neurons projecting from auditory to visual areas is found in adult cats bilaterally enucleated at birth, which suggests that the postnatal elimination of the auditory-to-visual projection is independent of visual experience and more generally of information coming from the retina.

Effects of neurotrophins on synaptic protein expression in the visual cortex of dark reared rats.

Total lack of visual experience [dark rearing (DR)] is known to prolong the critical period and delay development of sensory functions in mammalian visual cortex. Recent results show that neurotrophins (NTs) counteract the effects of DR on functional properties of visual cortical cells and exert a strong control on critical period duration. NTs are known to modulate the development and synaptic efficacy of neurotransmitter systems that are affected by DR. However, it is still unknown whether the actions of NTs in dark-reared animals involve interaction with neurotransmitter systems. We have studied the effects of DR on the expression of key molecules in the glutamatergic and GABAergic systems in control and NT-treated animals. We have found that DR reduced the expression of the NMDA receptor 2A subunit and its associated protein PSD-95 (postsynaptic density-95), of GRIP (AMPA glutamate receptor interacting protein), and of the biosynthetic enzyme GAD (glutamic acid decarboxylase). Returning dark-reared animals to light for 2 hr restored normal expression of the above-mentioned proteins almost completely. NT treatment specifically counteracts DR effects; NGF acts primarily on the NMDA system, whereas BDNF acts primarily on the GABAergic system. Finally, the action of NT4 seems to involve both excitatory and inhibitory systems. These data demonstrate that different NTs counteract DR effects by modulating the expression of key molecules of the excitatory and inhibitory neurotransmitter systems

Contrast sensitivity threshold measured by sweep-visual evoked potential in term and preterm infants at 3 and 10 months of age

Although healthy preterm infants frequently seem to be more attentive to visual stimuli and to fix on them longer than full-term infants, no difference in visual acuity has been reported compared to term infants. We evaluated the contrast sensitivity (CS) function of term (N = 5) and healthy preterm (N = 11) infants at 3 and 10 months of life using sweep-visual evoked potentials. Two spatial frequencies were studied: low (0.2 cycles per degrees, cpd) and medium (4.0 cpd). The mean contrast sensitivity (expressed in percentage of contrast) of the preterm infants at 3 months was 55.4 for the low spatial frequency (0.2 cpd) and 43.4 for the medium spatial frequency (4.0 cpd). At 10 months the low spatial CS was 52.7 and the medium spatial CS was 9.9. The results for the term infants at 3 months were 55.1 for the low spatial frequency and 34.5 for the medium spatial frequency. At 10 months the equivalent values were 54.3 and 14.4, respectively. No difference was found using the Mann-Whitney rank sum T-test between term and preterm infants for the low frequency at 3 or 10 months or for the medium spatial frequency at 3 or 10 months. The development of CS for the medium spatial frequency was equally fast for term and preterm infants. As also observed for visual acuity, CS was equivalent among term and preterm infants, suggesting that visual experience does not modify the development of the primary visual pathway. An earlier development of synapses in higher cortical visual areas of preterm infants could explain the better use of visual information observed behaviorally in these infants.

Orientation-selective functional magnetic resonance imaging adaptation in primary visual cortex revisited

The processing of orientations is at the core of our visual experience. Orientation selectivity in human visual cortex has been inferred from psychophysical experiments and more recently demonstrated with functional magnetic resonance imaging (fMRI). One method to identify orientation-selective responses is fMRI adaptation, in which two stimuli—either with the same or with different orientations—are presented successively. A region containing orientation-selective neurons should demonstrate an adapted response to the “same orientation” condition in contrast to the “different orientation” condition. So far, human primary visual cortex (V1) showed orientation-selective fMRI adaptation only in experimental designs using prolonged pre-adaptation periods (∼40 s) in combination with top-up stimuli that are thought to maintain the adapted level. This finding has led to the notion that orientation-selective short-term adaptation in V1 (but not V2 or V3) cannot be demonstrated using fMRI. The present study aimed at re-evaluating this question by testing three differently timed adaptation designs. With the use of a more sensitive analysis technique, we show robust orientation-selective fMRI adaptation in V1 evoked by a short-term adaptation design.

Analysis of quality of experience in 3D video systems

Esta tesis presenta un estudio exhaustivo sobre la evaluación de la calidad de experiencia (QoE, del inglés Quality of Experience) percibida por los usuarios de sistemas de vídeo 3D, analizando el impacto de los efectos introducidos por todos los elementos de la cadena de procesamiento de vídeo 3D. Por lo tanto, se presentan varias pruebas de evaluación subjetiva específicamente diseñadas para evaluar los sistemas considerados, teniendo en cuenta todos los factores perceptuales relacionados con la experiencia visual tridimensional, tales como la percepción de profundidad y la molestia visual. Concretamente, se describe un test subjetivo basado en la evaluación de degradaciones típicas que pueden aparecer en el proceso de creación de contenidos de vídeo 3D, por ejemplo debidas a calibraciones incorrectas de las cámaras o a algoritmos de procesamiento de la señal de vídeo (p. ej., conversión de 2D a 3D). Además, se presenta el proceso de generación de una base de datos de vídeos estereoscópicos de alta calidad, disponible gratuitamente para la comunidad investigadora y que ha sido utilizada ampliamente en diferentes trabajos relacionados con vídeo 3D. Asimismo, se presenta otro estudio subjetivo, realizado entre varios laboratorios, con el que se analiza el impacto de degradaciones causadas por la codificación de vídeo, así como diversos formatos de representación de vídeo 3D. Igualmente, se describen tres pruebas subjetivas centradas en el estudio de posibles efectos causados por la transmisión de vídeo 3D a través de redes de televisión sobre IP (IPTV, del inglés Internet Protocol Television) y de sistemas de streaming adaptativo de vídeo. Para estos casos, se ha propuesto una innovadora metodología de evaluación subjetiva de calidad vídeo, denominada Content-Immersive Evaluation of Transmission Impairments (CIETI), diseñada específicamente para evaluar eventos de transmisión simulando condiciones realistas de visualización de vídeo en ámbitos domésticos, con el fin de obtener conclusiones más representativas sobre la experiencia visual de los usuarios finales. Finalmente, se exponen dos experimentos subjetivos comparando varias tecnologías actuales de televisores 3D disponibles en el mercado de consumo y evaluando factores perceptuales de sistemas Super Multiview Video (SMV), previstos a ser la tecnología futura de televisores 3D de consumo, gracias a una prometedora visualización de contenido 3D sin necesidad de gafas específicas. El trabajo presentado en esta tesis ha permitido entender los factores perceptuales y técnicos relacionados con el procesamiento y visualización de contenidos de vídeo 3D, que pueden ser de utilidad en el desarrollo de nuevas tecnologías y técnicas de evaluación de la QoE, tanto metodologías subjetivas como métricas objetivas. ABSTRACT This thesis presents a comprehensive study of the evaluation of the Quality of Experience (QoE) perceived by the users of 3D video systems, analyzing the impact of effects introduced by all the elements of the 3D video processing chain. Therefore, various subjective assessment tests are presented, particularly designed to evaluate the systems under consideration, and taking into account all the perceptual factors related to the 3D visual experience, such as depth perception and visual discomfort. In particular, a subjective test is presented, based on evaluating typical degradations that may appear during the content creation, for instance due to incorrect camera calibration or video processing algorithms (e.g., 2D to 3D conversion). Moreover, the process of generation of a high-quality dataset of 3D stereoscopic videos is described, which is freely available for the research community, and has been already widely used in different works related with 3D video. In addition, another inter-laboratory subjective study is presented analyzing the impact of coding impairments and representation formats of stereoscopic video. Also, three subjective tests are presented studying the effects of transmission events that take place in Internet Protocol Television (IPTV) networks and adaptive streaming scenarios for 3D video. For these cases, a novel subjective evaluation methodology, called Content-Immersive Evaluation of Transmission Impairments (CIETI), was proposed, which was especially designed to evaluate transmission events simulating realistic home-viewing conditions, to obtain more representative conclusions about the visual experience of the end users. Finally, two subjective experiments are exposed comparing various current 3D displays available in the consumer market, and evaluating perceptual factors of Super Multiview Video (SMV) systems, expected to be the future technology for consumer 3D displays thanks to a promising visualization of 3D content without specific glasses. The work presented in this thesis has allowed to understand perceptual and technical factors related to the processing and visualization of 3D video content, which may be useful in the development of new technologies and approaches for QoE evaluation, both subjective methodologies and objective metrics.

Time-series Luminance Distribution Maps: implementation of annual daylight simulation methods for occupant visual comfort analysis

Thesis (Master's)--University of Washington, 2016-06

La gárgola en la cultura visual actual

La gárgola ha poblado desde antiguo las cornisas de los edificios religiosos y civiles, sirviendo de evacuación para el agua y para diferentes misiones según las distintas interpretaciones. Su gran componente simbólico hace de ella un reclamo muy interesante para el mundo audiovisual actual. De entre las manifestaciones encontradas, estudiaremos la visión de la gárgola en estos medios y valoraremos su adecuación o no a la visión medieval. Mediante la aparición de la gárgola medieval en el mundo actual en forma de personajes y ornamentos, y gracias a la aplicación de imágenes actuales a las nuevas gárgolas, podemos realizar un recorrido visual y simbólico alrededor de las distintas visiones que la cultura actual realiza de ellas. Pero no podemos quedarnos ahí, es necesario relacionar estas nuevas visiones y significaciones con las originales, para establecer si se ha hecho un uso correcto de la imagen, o se ha roto la estructura del símbolo primigenio.

Visual Insight in Geometry

According to a traditional rationalist proposal, it is possible to attain knowledge of certain necessary truths by means of insight—an epistemic mental act that combines the 'presentational' character of perception with the a priori status usually reserved for discursive reasoning. In this dissertation, I defend the insight proposal in relation to a specific subject matter: elementary Euclidean plane geometry, as set out in Book I of Euclid's Elements. In particular, I argue that visualizations and visual experiences of diagrams allow human subjects to grasp truths of geometry by means of visual insight. In the first two chapters, I provide an initial defense of the geometrical insight proposal, drawing on a novel interpretation of Plato's Meno to motivate the view and to reply to some objections. In the remaining three chapters, I provide an account of the psychological underpinnings of geometrical insight, a task that requires considering the psychology of visual imagery alongside the details of Euclid's geometrical system. One important challenge is to explain how basic features of human visual representations can serve to ground our intuitive grasp of Euclid's postulates and other initial assumptions. A second challenge is to explain how we are able to grasp general theorems by considering diagrams that depict only special cases. I argue that both of these challenges can be met by an account that regards geometrical insight as based in visual experiences involving the combined deployment of two varieties of 'dynamic' visual imagery: one that allows the subject to visually rehearse spatial transformations of a figure's parts, and another that allows the subject to entertain alternative ways of structurally integrating the figure as a whole. It is the interplay between these two forms of dynamic imagery that enables a visual experience of a diagram, suitably animated in visual imagination, to justify belief in the propositions of Euclid’s geometry. The upshot is a novel dynamic imagery account that explains how intuitive knowledge of elementary Euclidean plane geometry can be understood as grounded in visual insight.

Is there a common factor for vision?

In cognition, common factors play a crucial role. For example, different types of intelligence are highly correlated, pointing to a common factor, which is often called g. One might expect that a similar common factor would also exist for vision. Surprisingly, no one in the field has addressed this issue. Here, we provide the first evidence that there is no common factor for vision. We tested 40 healthy students' performance in six basic visual paradigms: visual acuity, vernier discrimination, two visual backward masking paradigms, Gabor detection, and bisection discrimination. One might expect that performance levels on these tasks would be highly correlated because some individuals generally have better vision than others due to superior optics, better retinal or cortical processing, or enriched visual experience. However, only four out of 15 correlations were significant, two of which were nontrivial. These results cannot be explained by high intraobserver variability or ceiling effects because test-retest reliability was high and the variance in our student population is commensurate with that from other studies with well-sighted populations. Using a variety of tests (e.g., principal components analysis, Bayes theorem, test-retest reliability), we show the robustness of our null results. We suggest that neuroplasticity operates during everyday experience to generate marked individual differences. Our results apply only to the normally sighted population (i.e., restricted range sampling). For the entire population, including those with degenerate vision, we expect different results.

Quand l’action surpasse la perception : rôle de la vision et de la proprioception dans la perception et le contrôle en temps réel de l’orientation spatiale de la main

Cette recherche a pour but d’évaluer le rôle de la vision et de la proprioception pour la perception et le contrôle de l’orientation spatiale de la main chez l’humain. L’orientation spatiale de la main est une composante importante des mouvements d’atteinte et de saisie. Toutefois, peu d’attention a été portée à l’étude de l’orientation spatiale de la main dans la littérature. À notre connaissance, cette étude est la première à évaluer spécifiquement l’influence des informations sensorielles et de l’expérience visuelle pour la perception et le contrôle en temps réel de l'orientation spatiale de la main pendant le mouvement d’atteinte naturel vers une cible stationnaire. Le premier objectif était d’étudier la contribution de la vision et de la proprioception dans des tâches de perception et de mouvement d’orientation de la main. Dans la tâche de perception (orientation-matching task), les sujets devaient passivement ou activement aligner une poignée de forme rectangulaire avec une cible fixée dans différentes orientations. Les rotations de l’avant-bras et du poignet étaient soit imposées par l’expérimentateur, soit effectuées par les sujets. Dans la tâche de mouvement d’orientation et d’atteinte simultanées (letter posting task 1), les sujets ont réalisé des mouvements d’atteinte et de rotation simultanées de la main afin d’insérer la poignée rectangulaire dans une fente fixée dans les mêmes orientations. Les tâches ont été réalisées dans différentes conditions sensorielles où l’information visuelle de la cible et de la main était manipulée. Dans la tâche perceptive, une augmentation des erreurs d’orientation de la main a été observée avec le retrait des informations visuelles concernant la cible et/ou ou la main. Lorsque la vision de la main n’était pas permise, il a généralement été observé que les erreurs d’orientation de la main augmentaient avec le degré de rotation nécessaire pour aligner la main et la cible. Dans la tâche de mouvement d’orientation et d’atteinte simultanées, les erreurs ont également augmenté avec le retrait des informations visuelles. Toutefois, les patrons d’erreurs étaient différents de ceux observés dans la tâche de perception, et les erreurs d’orientation n’ont pas augmenté avec le degré de rotation nécessaire pour insérer la poignée dans la fente. En absence de vision de la main, il a été observé que les erreurs d’orientation étaient plus petites dans la tâche de mouvement que de perception, suggérant l’implication de la proprioception pour le contrôle de l’orientation spatiale de la main lors des mouvements d’orientation et d’atteinte simultanées. Le deuxième objectif de cette recherche était d’étudier l’influence de la vision et de la proprioception dans le contrôle en temps réel de l’orientation spatiale de la main. Dans une tâche d’orientation de la main suivie d’une atteinte manuelle (letter posting task 2), les sujets devaient d’abord aligner l’orientation de la même poignée avec la fente fixée dans les mêmes orientations, puis réaliser un mouvement d’atteinte sans modifier l’orientation initiale de la main. Une augmentation des erreurs initiales et finales a été observée avec le retrait des informations visuelles. Malgré la consigne de ne pas changer l’orientation initiale de la main, une diminution des erreurs d’orientation a généralement été observée suite au mouvement d’atteinte, dans toutes les conditions sensorielles testées. Cette tendance n’a pas été observée lorsqu’aucune cible explicite n’était présentée et que les sujets devaient conserver l’orientation de départ de la main pendant le mouvement d’atteinte (mouvement intransitif; letter-posting task 3). La diminution des erreurs pendant l’atteinte manuelle transitive vers une cible explicite (letter-posting task 2), malgré la consigne de ne pas changer l’orientation de la main pendant le mouvement, suggère un mécanisme de corrections automatiques pour le contrôle en temps réel de l’orientation spatiale de la main pendant le mouvement d’atteinte naturel vers une cible stationnaire. Le troisième objectif de cette recherche était d’évaluer la contribution de l’expérience visuelle pour la perception et le contrôle de l’orientation spatiale de la main. Des sujets aveugles ont été testés dans les mêmes tâches de perception et de mouvement. De manière générale, les sujets aveugles ont présenté les mêmes tendances que les sujets voyants testés dans la condition proprioceptive (sans vision), suggérant que l’expérience visuelle n’est pas nécessaire pour le développement d’un mécanisme de correction en temps réel de l’orientation spatiale de la main basé sur la proprioception.