Virus diseases of sugar beets in California /

Mode of access: Internet.

Virus diseases of chickpeas and pulse crops in Australia

Accurate identification of viruses is critical for resistance breeding and for development of management strategies. To this end, we are developing PCR diagnostics for the luteoviruses / poleroviruses that commonly affect chickpea and pulse crops in Australia. This is helping to overcome the shortfalls in virus identifications that often result from cross reactions of viruses to some antibodies. We compared these PCR tests with antibody based Tissue blot immune-assay (TBIA) in virus surveys of chickpea and pulse crops from eastern Australia. We used a multiplex PCR for Beet western yellows virus (BWYV), Bean leaf roll virus (BLRV), Phasey bean virus (PhBV – a new polerovirus species) and Soybean dwarf virus (SbDV) to investigate the importance of each virus and their host range from different locations. Important alternative hosts included Malva parviflora which was commonly found to be infected with BWYV from many locations and Medicago polymorpha was a host for BLRV, PhBV and SbDV. Using the virus species-specific PCR, 49 virus affected plants (mostly crop plants) from surveys in 2013 were screened, revealing the following infections; 38 SbDV, 5 PhBV, 3 BWYV, 2 BLRV and 1 mixed SbDV/BWYV. From the 45 samples that were not BWYV by PCR, 33 were false-positives in the BWYV TBIA. This demonstrates the BWYV antibody used was not useful for identifying BWYV and PCR indicated that SbDV was the dominant virus from the samples tested from the 2013 season. Preliminary results from the 2014 season indicate a significant change, with SbDV being only a minor component of the total virus population. Further work to clarify the Australian luteovirus complex through molecular techniques is in progress.

Injurious insects and diseases of plants with remedial measures for the same /

"New series" vol. II, no. 1.

How to protect plants. A guide to the control of pests and diseases of plants in Hawaii.

Mode of access: Internet.

Diseases of plants.

Mode of access: Internet.

The leafhopper vectors of phytopathogenic viruses (Homoptera, Cicadellidae); taxonomy, biology, and virus transmission,

Bibliography: p. 318-368.

Bioassay of clover wound tumor virus and the mycoplasmalike organisms of peach Western X and aster yellows /

Literature cited: p. 28-32.

Genotypic comparison of Pantoea agglomerans plant and clinical strains

Pantoea agglomerans strains are among the most promising biocontrol agents for avariety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistichuman pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting.Results: Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Conclusion: Taxonomic mischaracterization was identified as a major problem with P.agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified whichmay be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in biosafety assessment of beneficial strains in this species

Genotypic comparison of Pantoea agglomerans plant and clinical strains

Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting. Results: Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Conclusion: Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in biosafety assessment of beneficial strains in this species

Potyvirus: caracterização parcial de espécies em plantas daninhas associadas a cultura do pimentão, avaliação de genótipos de alface e análise subcelular do eIF4E e de proteínas do Lettuce mosaic virus

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Ocorrência de viroses em mandioquinha-salsa (Arracacia xanthorrhiza brancroft) nas principais regiões produtoras do Brasil

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Viroses do alho: métodos de diagnose e degenerescência do alho semente livre de vírus

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Doenças do pimentão em regiões produtoras do Equador: identificação e manejo da pata seca e ocorrência de viroses

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Bulletin.

No. 1-2 published combined, but with sequential page numbering.

Distribution of Sugarcane mosaic virus in sugarcane plants

Variation in the concentration of virus in different parts of the plant has implications for virus-indexing programs. To allow more reliable detection of Sugarcane mosaic virus (SCMV), the distribution of the virus in sugarcane plants after artificial inoculation was studied using a reverse transcription polymerase chain reaction (RT-PCR) assay. Leaves of susceptible and moderately resistant sugarcane were mechanically inoculated with SCMV 6 weeks after planting. Weekly for 8 weeks after inoculation, plants were examined for mosaic symptoms and samples of leaves, roots and tillers were tested by RT-PCR to detect virus. SCMV moved from the point of inoculation to younger leaves, roots and tillers and eventually to leaves that emerged prior to inoculation. The pattern of SCMV distribution in moderately resistant and susceptible cultivars was not substantially different. However, the virus moved more slowly in the moderately resistant than in the susceptible cultivar. Young leaves proved to be the most suitable tissue for testing.