Trypanosoma cruzi: modification of alkaline phosphatase activity induced by trypomastigotes in cultured human placental villi

Human term placental villi cultured ''in vitro" were maintained with bloodstream forms of Trypanosoma cruzi during various periods of time. Two different concentrations of the parasite were employed. Controls contained no T. cruzi. The alkaline phosphatase activity was determined in placental villi by electron microscopy and its specific activity in the culture medium by biochemical methods. Results showed that the hemoflagellate produces a significant decrease in enzyme activity as shown by both ultracytochemical and specific activity studies and this activity was lower in cultures with high doses of parasites. The above results indicate that the reduction in enzyme activity coincides with the time of penetration and proliferation of T. cruzi in mammalian cells. These changes may represent an interaction between human trophoblast and T. cruzi.

[44 phot. de Norvège, de Bergen au Varanger fjord, par P.C.J. Villié Moriamez, prises en mai-juin 1881, don G. Pouchet en 1886]

Donateur : Pouchet, H. Ch. Georges (1833-1894)

Viisi vuotta eläinlääkkeiden haittavaikutusraportointia Lääkelaitokselle

Summary: Five years of adverse reaction reporting on veterinary medicinal products

Matkailun villi länsi Arizonan Tombstonessa

English abstract: Touristic wild west in Tombstone, Arizona

Viisi suurta persoonallisuuden piirrettä luottamuksen ja itsetunnon selittäjinä

Summary: How the big five personality traits explain trust and self-esteem