Vertical electric field stimulated neural cell functionality on porous amorphous carbon electrodes

We demonstrate the efficacy of amorphous macroporous carbon substrates as electrodes to support neuronal cell proliferation and differentiation in electric field mediated culture conditions. The electric field was applied perpendicular to carbon substrate electrode, while growing mouse neuroblastoma (N2a) cells in vitro. The placement of the second electrode outside of the cell culture medium allows the investigation of cell response to electric field without the concurrent complexities of submerged electrodes such as potentially toxic electrode reactions, electro-kinetic flows and charge transfer (electrical current) in the cell medium. The macroporous carbon electrodes are uniquely characterized by a higher specific charge storage capacity (0.2 mC/cm(2)) and low impedance (3.3 k Omega at 1 kHz). The optimal window of electric field stimulation for better cell viability and neurite outgrowth is established. When a uniform or a gradient electric field was applied perpendicular to the amorphous carbon substrate, it was found that the N2a cell viability and neurite length were higher at low electric field strengths (<= 2.5 V/cm) compared to that measured without an applied field (0 V/cm). While the cell viability was assessed by two complementary biochemical assays (MTT and LDH), the differentiation was studied by indirect immunostaining. Overall, the results of the present study unambiguously establish the uniform/gradient vertical electric field based culture protocol to either enhance or to restrict neurite outgrowth respectively at lower or higher field strengths, when neuroblastoma cells are cultured on porous glassy carbon electrodes having a desired combination of electrochemical properties. (C) 2013 Elsevier Ltd. All rights reserved.

Vertical electric field induced bacterial growth inactivation on amorphous carbon electrodes

The objective of the present work is to understand the vertical electric field stimulation of the bacterial cells, when grown on amorphous carbon substrates in vitro. In particular, the antibacterial activity against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli are studied using MTTassay, live/dead assay and inner membrane permeabilization assays. In our experiments, the carbon substrate acts as one electrode and the counter electrode is positioned outside the culture medium, thus suppressing the current, electrokinetic motions and chemical reactions. Guided by similar experiments conducted in our group on neuroblastoma cells, the present experimental results further establish the interdependence of field strength and exposure duration towards bacterial growth inactivation in vitro. Importantly, significant reduction in bacterial viability was recorded at the 2.5 V/cm electric field stimulation conditions, which does not reduce the neural cell viability to any significant extent on an identical substrate. Following electrical stimulation, the bacterial growth is significantly inhibited for S. aureus bacterial strain in an exposure time dependent manner. In summary, our experiments establish the effectiveness of the vertical electric field towards bacterial growth inactivation on amorphous carbon substrates, which is a cell type dependent phenomenon (Gram-positive vs. Gram-negative). (C) 2014 Elsevier Ltd. All rights reserved.

Electronic structure of coupled vertically stacked self-assembled InAs quantum disks in a vertical electric field

In the framework of the effective-mass and adiabatic approximations, by setting the effective-mass of electron in the quantum disks (QDs) different from that in the potential barrier material, we make some improvements in the calculation of the electronic energy levels of vertically stacked self-assembled InAs QD. Comparing with the results when an empirical value was adopted as the effective-mass of electron of the system, we can see that the higher levels become heightened. Furthermore, the Stark shifts of the system of different methods are compared. The Stark shifts of holes are also studied. The vertical electric field changes the splitting between the symmetric level and the antisymmetric one for the same angular momentum. (C) 2003 Elsevier Ltd. All rights reserved.

Transport properties through quantum dot in a vertical electric field

The transport properties through a quantum dot are calculated using the recursion method. The results show that the electric fields can move the conductive peaks along the high- and low-energies. The electric field changes the intensity of conductance slightly. Our theoretical results should be useful for researching and making low-dimensional semiconductor optoelectronic devices. (C) 2002 Elsevier Science B.V. All rights reserved.

Transport properties through quantum dot in a vertical electric field

The transport properties through a quantum dot are calculated using the recursion method. The results show that the electric fields can move the conductive peaks along the high- and low-energies. The electric field changes the intensity of conductance slightly. Our theoretical results should be useful for researching and making low-dimensional semiconductor optoelectronic devices. (C) 2002 Elsevier Science B.V. All rights reserved.

On vertical electric fields at lunar magnetic anomalies

We study the interaction between a magnetic dipole mimicking the Gerasimovich magnetic anomaly on the lunar surface and the solar wind in a self-consistent 3-D quasi-neutral hybrid simulation where ions are modeled as particles and electrons as a charge-neutralizing fluid. Especially, we consider the origin of the recently observed electric potentials at lunar magnetic anomalies. An antimoonward Hall electric field forms in our simulation resulting in a potential difference of <300V on the lunar surface, in which the value is similar to observations. Since the hybrid model assumes charge neutrality, our results suggest that the electric potentials at lunar magnetic anomalies can be formed by decoupling of ion and electron motion even without charge separation.

Investigación geofísica de los paleovalles pleistocenos del NE de Cataluña: el ejemplo de la Riera de Tossa (Costa Brava meridional)

The geometry of Riera de Tossa (Costa Brava, Catalonia) Pleistocene paleovalley has been studied by two geophysical prospecting methods: vertical electric sounding (VES-DC) and seismic refraction. The results show the existence of a sudden slope change of the thalweg that permits to differentiate two sections where erosion processes operated in unequal intensity. Based on geological and geomorphological available data, it can be inferred that this slope rupture is caused by the litological control imposed by the alineation of resistant materials, which limited the headward erosion extend of the creek during the Upper Pleistocene

O emprego de métodos geofísicos na fase de investigação confirmatória em cemitérios contaminados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação da eletrorresistividade na caracterização do meio hidrogeológico na cidade de JI-paraná (RO)

The Ji-Paraná city (RO) it doesn't possess public system of collection and treatment of sewers, being the waters residuárias produced by the local population thrown at sewages. Traditionally, many inhabitants use wells amazon extracted underground water or tubular shallow in the urban zone. The study accomplished in the Nova Brasília neighborhood for Silva (2009) revealed that the local aquifer is strongly contaminated for nitrate, originated of the decomposition of the organic matter deposited at the sewages local maidservants. With the objective of detecting areas with high concentrations originating from organic compositions of septic sewages, geophysical risings were accomplished, later related with analyses physical-chemistries in samples of groundwaters obtained in several wells installed in the Nova Brasília neighborhood, besides of soil samples descriptions in zone not saturated obtained in wells. The results obtained by the geophysical rehearsals they reveal that the polluting feather not migrates through the zone saturated, arriving with relative easiness to the aquifer, reaching in some points, superior depth to 34 m reached by the geoelectrical profiling.

Aplicação do método eletromagnético transiente em área contaminada sob atenuação natural

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Aplicação do método da eletrorresistividade na investigação e mapeamento da contaminação por cemitérios: o exemplo do cemitério da Vila Rezende, Piracicaba/SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Geofísica aplicada no monitoramento de área de disposição de resíduos sólidos domiciliares

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo hidrofaciológico do aqüífero Rio Claro no município de Rio Claro - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da contaminação do lençol freático através da integração de técnicas geofísicas e geoquímicas em Ji-Paraná-RO

Pós-graduação em Geociências e Meio Ambiente - IGCE

CARACTERIZAÇÃO ESTRUTURAL E GEOFÍSICA DA LIXEIRA DE PORTO VELHO (RO)

O município de Porto Velho (RO) gera em torno de 220 toneladas de lixo urbano por dia, os quais são dispostos em área de “lixeira” desde 1993. Este trabalho apresenta os resultados da aplicação do método geofísico da eletrorresistividade, através das técnicas da sondagem elétrica vertical e imageamento elétrico (arranjos Schlumberger e dipolo-dipolo) que foram executadas na área da lixeira da cidade de Porto Velho (RO), com o objetivo de identificar a contaminação do solo e das águas subterrâneas. A geologia sobre a qual está assentado o aterro de Porto Velho é constituída por sedimentos do pleistoceno (argilo-arenosos com concreções lateríticas) assentados sobre rochas graníticas – fraturadas - da Formação Santo Antônio. Palavras-chave: Eletrorresistividade, imageamento elétrico, sondagem elétrica vertical, resíduos sólidos urbanos.