101 resultados para Ultraestrutura
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Foram estudados os efeitos da glutamina, dos ácidos graxos poli-insaturados e da parede celular de levedura (PCL) sobre a estrutura e ultraestrutura do intestino delgado e o desempenho de leitões. Foram utilizados 45 leitões, desmamados aos 21 dias de idade, para testar os seguintes tratamentos: T1 - dieta basal; T2 - dieta basal + 1% de glutamina; T3 - dieta basal + 0,2% de PCL; T4 - dieta basal + 5% de óleo de peixe. Nos dias sete e 14 pós-desmame, foram abatidos cinco leitões de cada tratamento. Os aditivos testados não alteraram a altura e a densidade dos vilos nem a profundidade das criptas do intestino delgado. Foi observado efeito de idade, mostrando redução na altura e na densidade dos vilos e na profundidade das criptas após o desmame. No duodeno e jejuno, foram observados maiores valores de relação vilo:cripta, que aumentaram com a idade pós-desmame. Ocorreram redução da altura dos microvilos do duodeno aos sete dias e aumento da largura dos microvilos do jejuno aos 14 dias pós-desmame. A área de superfície apical dos enterócitos não foi alterada pelos fatores estudados. Os aditivos estudados não foram eficientes em prevenir a atrofia da mucosa intestinal do jejuno, ao não interferir na sua ultraestrutura. Os aditivos incluídos na dieta não influenciaram o desempenho dos leitões no pós-desmame.
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The aim of this study was to describe the morphology, morphometry and ultrastructure of segments of thoracic and abdominal aorta portions in four male and female paca (Cuniculus paca). Parts of the segments were examined by light microscopy and part by scanning electron microscopy. Thickness measurements of the tunica intima and media complex and tunica adventitia of the aorta were taken. In all animals the thickness values for the tunica intima and media complex of the cranial thoracic aorta were significantly higher (mean: 702.19 mu m) when compared to the values of other aortic segments analyzed (means: 354.18 mu m; 243.55 mu m). The layers of the vessel walls show variations in structure and thickness, presumably due to an adaptation to functionaldemand.
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Two experiments (experiment 1 - conventional feeding and experiment 2 - feeding of the sows, starting at 109 days of pregnancy and during lactation, and the lactating piglets, with rations without soybean meal) were conducted to evaluate the effects of sources of protein (soybean meal-SBM, extruded semi-integral soybean-ESISB, and milk products-MP) on the intestinal morphology of piglets slaughtered at the 0, 5 and 15 days later at weaning. Twenty-seven piglets weaned with 25 days of age were used, being nine piglets slaughter in each slaughtering age. A 3 ×3 factorial (3 diets × 3 slaughtering ages) was studied, according to a randomized block design. The results showed that animals receiving SBM, presented lower values of villi height and villus: crypt ratio when compared to those that received ESISB or MP. The villi height and the villus: crypt ratio were not affected by the differential feeding of the sows and of the piglets before the weaning. The depth of the crypt was not affected by diets or slaughtering ages in none of the segments studied being, however, smaller in the duodenum and bigger in the jejunum and ileo of the animals of exp. 2. It was concluded that the SBM was the affected the height of the villi and the relationship villi: Crypt negatively after it weans. The intestinal morphology was more affected at five days, showing signals of recovering at 15 days after weaning and that the feeding of the sows did not affect the morphology of the segments of the thin intestine.
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Considering the lack of detailed information about the morphology of paca, which is the second largest rodent of Brazilian's fauna, with excellent meat quality and, due to the importance of the common integument, we described the morphology, ultrastructure and morphometry of the skin of eight pacas (Cuniculus paca), males and females, through comparative analysis of skin's segments of the cervical, dorsal and medial carpal portions. Macroscopic characteristics of the coat were studied. Part of the segments of cutaneous regions was analyzed by light microscopy, and the other part by scanning electron microscopy. We measured the thickness of dermis, epidermis, stratum corneum, the profiles of the collagen fibers of reticular dermis and the area of filled sebaceous gland cells. The results were analyzed by descriptive statistics and T test (p<0.001). The color of the coat is reddish brown with bristles arranged in groups. The architecture of the skin and skin appendages resembles those of mammals in general, although there are no sweat glands. According to the morphometric analysis, this study shows differences of the skin architecture between male and female pacas and also between various body regions in the same animal.
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The paca (Cuniculus paca) is the second largest rodent of the Brazilian fauna. The excellent meat quality of this specie encourages the development of their commercial production. Moreover, this animal can become a viable alternative for animal experimentation although there exists scarce detailed information concerning their morphology. Therefore the purpose of this study is to describe the morphology, morphometry and ultrastructure in segments of the cranial and caudal portions of vena cava in four adult males and females of Cuniculus paca from the squad of Wild Animals Sector of Animal Science Department of FCAV-Unesp. Parts of the segments were examined by light microscopy and part by scanning electron microscopy. Thickness measures of the tunica intima and media complex and tunica adventitia of the vena cava were taken and analyzed using T test (p<0.05). In vena cava the thickness values of the intima, media and adventitia, for all animals, were significantly higher in the cranial segment. The layers of the vessel walls showed variations in structure and thickness, presumably due to an adaptation to functional demand.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Genética - IBILCE
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC
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Pós-graduação em Biologia Geral e Aplicada - IBB
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Pós-graduação em Cirurgia Veterinária - FCAV
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The ultrastructure of spermatozoa of the Protothaca pectorina (Lamarck, 1818) is described. The most significam differences were found in the structure and arreangement of the acrosomes, with contains two types of material, one fibrilar, the other electrondense granular. It is concluded that sperm ultrastructure is of the primitive type.