De limitata regiminis forma

Invocatio: D.D.

Jornal da UNESP, 2016, ano XXXI, n. 321

Jornal elaborado pela Assessoria de Comunicação e Imprensa da Reitoria da UNESP

[4 phot. des monuments élevés par Jean Joseph de La Borde dans le parc de son château de Méréville (Seine-et-Oise), à la fin du XVIIIe siècle, à la mémoire de ses deux fils, de La Borde-Marchainville et de La Borde-Boutervilliers, officiers de l'Astrolabe, péris avec La Pérouse en 1786, ainsi qu'à la mémoire du capitaine Cook, don du prince Roland Bonaparte en 1896. Voir les CR. des séances de la S.G., 1896, p. 319-321]

Donateur : Bonaparte, Roland (1858-1924)

Production of Acetic Acid by Fermentation with Propionibacteria, HR-321, 1993

This project was undertaken jointly with a project supported by the Iowa Corn Promotion Board. Together the projects aimed at producing the organic acids, propionic acid and acetic acid, by fermentation. The impacts were to provide agriculturally-based alternatives to production of these acids, currently produced mainly as petrochemicals. The potentially high-demand use for acetic acid is as the "acetate" in Calcium Magnesium Acetate (CMA), the non-corrosive road deicer. Fermentation was, however, far from being an economically acceptable alternative. Gains were made in this work toward making this a feasible route. These advances included (1) development of a variant strain of propionibacteria capable of producing higher concentrations of acids; (2) comparison of conditions for several ways of cultivating free cells and establishment of the relative benefits of each; (3) achievement of the highest productivity in fermentations using immobilized cells; (4) identification of corn steep liquor as a lower cost substrate for the fermentation; (5) application of a membrane extraction system for acid recovery and reduction of product inhibition; and (6) initial use of more detailed economic analysis of process alternatives to guide in the identification of where the greatest payback potential is for future research. At this point, the fermentation route to these acids using the propionibacteria is technically feasible, but economically unfeasible. Future work with integration of the above process improvements can be expected to lead to further gains in economics. However, such work can not be expected to make CMA a less expensive deicer than common road salt.

Methionine-321 in the C-terminal alpha-helix of catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa is important for positive homotropic cooperativity.

Pseudomonas aeruginosa has a pair of distinct ornithine carbamoyltransferases. The anabolic ornithine carbamoyltransferase encoded by the argF gene catalyzes the formation of citrulline from ornithine and carbamoylphosphate. The catabolic ornithine carbamoyltransferase encoded by the arcB gene promotes the reverse reaction in vivo; although this enzyme can be assayed in vitro for citrulline synthesis, its unidirectionality in vivo is determined by its high concentration at half maximum velocity for carbamoylphosphate ([S]0.5) and high cooperativity toward this substrate. We have isolated mutant forms of catabolic ornithine carbamoyltransferase catalyzing the anabolic reaction in vivo. The corresponding arcB mutant alleles on a multicopy plasmid specifically suppressed an argF mutation of P. aeruginosa. Two new mutant enzymes were obtained. When methionine 321 was replaced by isoleucine, the mutant enzyme showed loss of homotropic cooperativity at physiological carbamoylphosphate concentrations. Substitution of glutamate 105 by lysine resulted in a partial loss of the sigmoidal response to increasing carbamoylphosphate concentrations. However, both mutant enzymes were still sensitive to the allosteric activator AMP and to the inhibitor spermidine. These results indicate that at least two residues of catabolic ornithine carbamoyltransferase are critically involved in positive carbamoylphosphate cooperativity: glutamate 105 (previously known to be important) and methionine 321. Mutational changes in either amino acid will affect the geometry of helix H2, which contains several residues required for carbamoylphosphate binding.

Bessat, Hubert (2010): Le patois et la vie traditionnelle aux Contamines-Montjoie. I. La nature, les activités agro-pastorales et forestières. Grenoble: ELLUG / Universitat de Grenoble, 321 p.

En aquesta monografia Hubert Bessat ens presenta la primera part del seu estudi sobre el parlar de les Contamines-Montjoie. El poble de les Contamines-Montjoie (departament de l"Alta Savoia) està situat al sud del Montblanc, en una vall oberta al nord, en el territori francoprovençal de França a tocar de la Vall d"Aosta, aquesta ja a Itàlia.

De nummis quibusdam antiquis, in Finlandia haud ita pridem repertis

Invocatio: I.N.J.C.

L'Étoile du Nord : opéra comique en trois actes. [Suivi de] Brin-d'amour : opérette. Livr. 321 / paroles de M. E. Scribe... ; par M. Achille Lafont...

Collection : Théâtre contemporain illustré ; 321e et 322e livraisons

Degradación a altas temperaturas de un acero inoxidable tipo AISI 321 en hornos industriales.

Tesis (Maestría en Ciencias de la Ingeniería Mecánica con Especialidad en Materiales) UANL, 2013.

Índice bibliográfico de la revista de educación de los números 321-323 y número extraordinario año 2000.

Incluye cuatro tipos de índices que facilitan la búsqueda de los artículos publicados en los números 321 a 323 y en el número extraordinario del año 2000. En el primer índice, los artículos se agrupan por notaciones de la Clasificación Decimal Universal (CDU) y en los siguientes por orden alfabético de autores, títulos y materias.