165 resultados para TCM
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Report on a review of selected general and application controls over the Iowa Department of Human Services’ Targeted Case Management System (TCM) for the period March 31, 2014 through April 18, 2014
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Pós-graduação em Alimentos e Nutrição - FCFAR
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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This study was carried out to assess the influence of bovine embryo culture medium Beltsville Agriculture Research Center (BARC), supplemented with FCS, BSA or PVA, on the in vitro oocyte maturation, evidenced by cleavage rate and blastocysts production at different developmental stages. Three experiments were performed, as follows: exp.1: addition of FCS to BARC medium at concentrations of 0, 5 and 10%; exp. 2: addition of BSA to BARC medium at concentrations of 0, 4 and 8 mg/ml; exp. 3: addition of PVA to BARC medium at concentrations of 0, 0.5 and 1.0 mg/ml. TCM 199 supplemented with bicarbonate, pyruvate, gentamicin sulfate, FSH, LH and FCS was used as control group. Oocytes obtained from cow ovaries at slaughterhouse were selected in PBS, and then matured in BARC medium supplemented with FSH, LH and gentamicin sulfate, according to the experimental design. Percoll gradient was used for sperm selection and TALP medium for IVF. In vitro embryo culture was in SOF-m medium; a humidified atmosphere with 5% CO2, in air, at 38.7oC was used for all steps. The number of oocytes reaching blastocyst, expanded blastocyst, and hatched blastocyt stages was recorded, respectively at 72 and 168 h post-insemination. ANOVA and Bonferroni t test were used to determine differences among groups. Differences of P<0.05 were taken as significant. Higher percentage (P<0.05) of cleaved oocytes was observed in group TCM + FCS than for the other groups matured in BARC supplemented with FCS or BSA, regardless the concentration used. However, the cleavage rate was similar between groups BARC plus PVA with 1 mg/ml (85.7%) and TCM + FCS (90.8%). Significant difference was found among groups for the production of blastocysts, with the control group yielding a higher number of blastocysts (results ranging from 47.4 to 51.4%, in comparison with groups using BARC + FCS (4.1 to 19.7%), BSA (1.4 to 5.6%) and PVA (5.7 to 10.6%). In conclusion, BARC medium supplemented with different macromolecules did not promote a beneficial effect on in vitro oocyte maturation, resulting in lower rate of cleavage and blastocyst production when compared with TCM + FCS medium.
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Desde que las Tecnologías de la Información y la Comunicación comenzaron a adquirir una gran importancia en la sociedad, uno de los principales objetivos ha sido conseguir que la información transmitida llegue en perfectas condiciones al receptor. Por este motivo, se hace necesario el desarrollo de nuevos sistemas de comunicación digital capaces de ofrecer una transmisión segura y fiable. Con el paso de los años, se han ido mejorando las características de los mismos, lo que significa importantes avances en la vida cotidiana. En este contexto, uno de los sistemas que más éxito ha tenido es la Modulación Reticulada con Codificación TCM, que aporta grandes ventajas en la comunicación digital, especialmente en los sistemas de banda estrecha. Este tipo de código de protección contra errores, basado en la codificación convolucional, se caracteriza por realizar la modulación y codificación en una sola función. Como consecuencia, se obtiene una mayor velocidad de transmisión de datos sin necesidad de incrementar el ancho de banda, a costa de pasar a una constelación superior. Con este Proyecto Fin de Grado se quiere analizar el comportamiento de la modulación TCM y cuáles son las ventajas que ofrece frente a otros sistemas similares. Se propone realizar cuatro simulaciones, que permitan visualizar diversas gráficas en las que se relacione la probabilidad de bit erróneo BER y la relación señal a ruido SNR. Además, con estas gráficas se puede determinar la ganancia que se obtiene con respecto a la probabilidad de bit erróneo teórica. Estos sistemas pasan de una modulación QPSK a una 8PSK o de una 8PSK a una 16QAM. Finalmente, se desarrolla un entorno gráfico de Matlab con el fin de proporcionar un sencillo manejo al usuario y una mayor interactividad. ABSTRACT. Since Information and Communication Technologies began to gain importance on society, one of the main objectives has been to achieve the transmitted information reaches the receiver perfectly. For this reason, it is necessary to develop new digital communication systems with the ability to offer a secure and reliable transmission. The systems characteristics have improved over the past years, what it means important progress in everyday life. In this context, one of the most successful systems is Trellis Coded Modulation TCM, that brings great advantages in terms of digital communications, especially narrowband systems. This kind of error correcting code, based on convolutional coding, is characterized by codifying and modulating at the same time. As a result, a higher data transmission speed is achieved without increasing bandwidth at the expense of using a superior modulation. The aim of this project is to analyze the TCM performance and the advantages it offers in comparison with other similar systems. Four simulations are proposed, that allows to display several graphics that show how the Bit Error Ratio BER and Signal Noise Ratio SNR are related. Furthermore, it is possible to calculate the coding gain. Finally, a Matlab graphic environment is designed in order to guarantee the interactivity with the final user.
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Texas Department of Transportation, Austin
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Universidade Estadual de Campinas . Faculdade de Educação Física
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Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10-9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM.
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Recently, conservationists have debated whether consumers of animal and plant potency products used to treat erectile dysfunction (ED) in traditional Chinese medicine (TCM) might be switching to Viagra, consequently consuming fewer of these animals and plants. To address this question, a survey examined the medical decisions of male consumers of TCM in Hong Kong who were over the age of 50. As predicted, these consumers reported selectively switching to Western medicines to treat ED, but not to treat other health ailments. These findings provide support for the possibility that Viagra may have conservation benefits for certain species.