Effect of gossypol on survival and reproduction of the zoophytophagous stinkbug Podisus nigrispinus (Dallas)

Effect of gossypol on survival and reproduction of the zoophytophagous stinkbug Podisus nigrispinus (Dallas). Gossypol is a sesquiterpene aldehyde found in cotton plants conferring resistance against herbivory. Although the effect of this sesquiterpenoid on insect pests of cotton is known, the interaction of this compound with zoophytophagous predators such as Podisus nigrispinus (Dallas) (Hemiptera, Pentatomidae) has not been studied so far. Thus, the objective of this study was to evaluate the effect of the purified gossypol on nymphs and adults of P. nigrispinus. Nymphs and adults of this predator were fed on Tenebrio molitor pupae and supplemented with solutions of gossypol at concentrations of 0.00, 0.05, 0.10, and 0.20% (w/v) during the nymphal and adult stages or, only during the adult stage of P. nigrispinus. The nymphal stage of the predator was, on average, two days longer when suplemmented with gossypol. Emerged adults had lower fecundity and egg hatching, especially at the highest gossypol concentration (0.20%) ingested during the nymphal and adult stages. However, this predator was not affected when it ingested the compound only during the adult stage. P. nigrispinus can have delayed nymphal development and lower reproductive performance when ingesting the gossypol during the nymphal and adult stages, but only at higher concentrations of gossypol than that produced by cotton plants.

Parasitismo em ovos de percevejos da soja Euschistus heros (Fabricius) e Piezodorus guildinii (Westwood) (Hemiptera: Pentatomidae) em São Gabriel do Oeste, MS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da ocorrência sazonal de percevejos em cinco espécies de maracujazeiro, utilizando dois métodos de amostragem

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of the insect growth regulator diflubenzuron on the predator Podisus nigrispinus (Heteroptera: Pentatomidae)

Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) is a common natural predator of defoliating caterpillars in agricultural and forest systems. Insecticides acting as growth regulators of insect pests can indirectly affect their predators through consumption of contaminated prey. We examined the reproductive performance of P. nigrispinus fed on caterpillars of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae) reared on soybean leaves exposed to the chitin synthesis inhibitor, diflubenzuron. Caterpillars of A. gemmatalis were fed for 12 h with treated soybean leaves and offered to adults of the predator P. nigrispinus over five consecutive days. The fertility of P. nigrispinus was reduced when feeding on diflubenzuron treated caterpillars, especially at the beginning of the reproductive period, but recovered 3 weeks later. The effects of diflubenzuron ingestion on the life table parameters of P. nigrispinus included an increase in the period taken to double the population size, and reductions in the intrinsic rate of population increase, generation duration, and net reproductive rate. Diflubenzuron therefore had an indirect negative effect on the reproduction and the population dynamics of the non-target predator P. nigrispinus. Clearly, its use in integrated pest management requires further evaluation.

Mating preferences and consequences of choosing sibling or non-sibling mates by females of the predator Podisus nigrispinus (Heteroptera: Pentatomidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae): efeito do alimento e da temperatura de armazenamento de ovos no desenvolvimento do inseto

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização biológico-comportamental de Podisus nigrispinus (Dallas, 1851) predando Plutella xylostella (L., 1758)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

cis-Jasmone e Tween 20 na indução de metabólitos secundários em soja e seus efeitos em insetos-praga

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Sperm depletion: a cost for single mated females of Podisus nigrispinus (Dallas, 1851) (Heteroptera: Pentatomidae)

Entre os heterópteros, o declínio da concentração de esperma pode ser um fator limitante para o sucesso reprodutivo desses insetos. Acasalamentos múltiplos conferem um reabastecimento de esperma e podem permitir um aumento do valor adaptativo das fêmeas. Neste estudo foi testada a hipótese de que a poliandria aumenta a viabilidade dos ovos. A longevidade de fêmeas de Podisus nigrispinus, bem como seus parâmetros reprodutivos em resposta a diferentes números de acasalamentos (0, 1, 2, 3 ou em coabitação com o mesmo macho) foi avaliada. Esse percevejo vem sendo usado em programas de controle biológico de pragas em reflorestamentos de Eucalipto no Brasil. Apesar da diminuição no tempo de sobrevivência das fêmeas, acasalamentos múltiplos mantiveram a viabilidade dos ovos e o período reprodutivo das fêmeas, permitindo um maior número de descendentes produzidos. Tais resultados indicam que um número mínimo de três acasalamentos antes da primeira postura permite uma maximização do sucesso reprodutivo dessa espécie.

Development of pachycoris torridus (hemiptera: scutelleridae) on jatropha curcas (euphorbiaceae), psidium cattleianum (myrtaceae) and aleurites fordii (euphorbiaceae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da migração de nifas de Scaptocoris carvalhoi Becker, 1967 (Hemiptera, Cydnidae) em plantas de importância econômica e invasora em teste com oportunidade de escolha

One of the factors that affect the control of brown - roots environmental bedbug is the wide availability of plant species. Thus, this study aimed to evaluate the migration of nymphs of the brown - roots between plant species of economic importance and invasive stinkbug. Eight species of plants have been established in a set formed by eight circular PVC containers, connected symmetrically with free communication to a central circular container. The experiment was arranged in a completely randomized design and consisted of eight treatments and six replications. The test evaluations were performed on 15o , 30o , 45o and 60o days after the release of the nymphs, by counting the insects that migrated to the roots of different plants. It was observed that by the 30o day of early release nymphs, no significant differences (P < 0,05) in the choices nymph by plants. B. alata at 15 days of initial release and B. alata and grass-dictyoneura at 30 days were plants that had higher migration. All counts were carried out on 45o and 60o days, the migration of U. brizantha cv. Marandu presented significantly higher than all other species tested plants nymphs.

The gastric caeca of pentatomids as a house for actinomycetes

Abstract Background Microbes are extensively associated with insects, playing key roles in insect defense, nutrition and reproduction. Most of the associations reported involve Proteobacteria. Despite the fact that Actinobacteria associated with insects were shown to produce antibiotic barriers against pathogens to the hosts or to their food and nutrients, there are few studies focusing on their association with insects. Thus, we surveyed the Actinobacteria diversity on a specific region of the midgut of seven species of stinkbugs (Hemiptera: Pentatomidae) known to carry a diversity of symbiotically-associated Proteobacteria. Results A total of 34 phylotypes were placed in 11 different Actinobacteria families. Dichelops melacanthus held the highest diversity with six actinobacteria families represented by nine phylotypes. Thyanta perditor (n = 7), Edessa meditabunda (n = 5), Loxa deducta (n = 4) and Pellaea stictica (n = 3) were all associated with three families. Piezodorus guildini (n = 3) and Nezara viridula (n = 3) had the lowest diversity, being associated with two (Propionibacteriaceae and Mycobacteriaceae) and one (Streptomyceataceae) families, respectively. Corynebacteriaceae and Mycobacteriaceae were the most common families with phylotypes from three different insect species each one. Conclusions Many phylotypes shared a low 16S rRNA gene similarity with their closest type strains and formed new phyletic lines on the periphery of several genera. This is a strong indicative that stinkbug caeca can harbor new species of actinobacteria, which might be derived from specific associations with the species of stinkbugs studied. Although the well-known role of actinobacteria as a source of biomolecules, the ecological features of these symbionts on the stinkbugs biology remain unknown.