Miller Family Papers - Accession 204 - M90 (115-116)

The Miller Family Papers consist of notes on the Miller, Cathcart, and Roddey families, genealogical data on the Lindsay, Stewart, and McCaughrin families, and an American Civil War reminiscence of William Joseph Miller entitled, “My Experience as a Soldier in the Confederate Army. Written at the Request of Barnette, My Only Living Daughter.” Miller served in the 12th Regiment, South Carolina Volunteers of the Confederate Army.

Association Between Polymorphisms in GRIK2 Gene and Obsessive-Compulsive Disorder: A Family-Based Study

Several studies support a genetic influence on obsessive-compulsive disorder (OCD) etiology. The role of glutamate as an important neurotransmitter affecting OCD pathophysiology has been supported by neuroimaging, animal model, medication, and initial candidate gene studies. Genes involved in glutamatergic pathways, such as the glutamate receptor, ionotropic, kainate 2 (GRIK2), have been associated with OCD in previous studies. This study examines GRIK2 as a candidate gene for OCD susceptibility in a family-based approach. Probands had full DSM-IV diagnostic criteria for OCD. Forty-seven OCD probands and their parents were recruited from tertiary care OCD specialty clinics from France and USA. Genotypes of single nucleotide polymorphism (SNP) markers and related haplotypes were analyzed using Haploview and FBAT software. The polymorphism at rs1556995 (P = 0.0027; permuted P-value = 0.03) was significantly associated with the presence of OCD. Also, the two marker haplotype rs1556995/rs1417182, was significantly associated with OCD (P = 0.0019, permuted P-value = 0.01). This study supports previously reported findings of association between proximal GRIK2 SNPs and OCD in a comprehensive evaluation of the gene. Further study with independent samples and larger sample sizes is required.

Ida Crawford Stewart Papers - Accession 487

The Stewart Papers consist of correspondence, newspaper articles, photographs, financial records, and other papers, relating to Stewart’s family history as well as such subjects as slavery, the American Civil War, Reconstruction in the South, and Mrs. Stewart’s professional career.

Classification of organisms previously reported as the SP and Stewart-Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group

To allow classification of bacteria previously reported as the SP group and the Stewart-Letscher group, 35 isolates from rodents (21), rabbits (eight), a dog and humans (five) were phenotypically and genotypically characterized. Comparison of partial rpoB sequences showed that 34 of the isolates were closely related, demonstrating at least 97.4 % similarity. 16S rRNA gene sequence comparison of 20 selected isolates confirmed the monophyly of the SP group and revealed 98.5 %-100 % similarity between isolates. A blast search using the 16S rRNA gene sequences showed that the highest similarity outside the SP group was 95.5 % to an unclassified rat isolate. The single strain, P625, representing the Stewart-Letscher group showed the highest 16S rRNA gene similarity (94.9-95.5 %) to members of the SP group. recN gene sequence analysis of 11 representative strains resulted in similarities of 97-100 % among the SP group strains, which showed 80 % sequence similarity to the Stewart-Letscher group strain. Sequence similarity values based on the recN gene, indicative for whole genome similarity, showed the SP group being clearly separated from established genera, whereas the Stewart-Letscher group strain was associated with the SP group. A new genus, Necropsobacter gen. nov., with only one species, Necropsobacter rosorum sp. nov., is proposed to include all members of the SP group. The new genus can be separated from existing genera of the family Pasteurellaceae by at least three phenotypic characters. The most characteristic properties of the new genus are that haemolysis is not observed on bovine blood agar, positive reactions are observed in the porphyrin test, acid is produced from (+)-L-arabinose, (+)-D-xylose, dulcitol, (+)-D-galactose, (+)-D-mannose, maltose and melibiose, and negative reactions are observed for symbiotic growth, urease, ornithine decarboxylase and indole. Previous publications have documented that both ubiquinones and demethylmenaquinone were produced by the proposed type strain of the new genus, Michel A/76(T), and that the major polyamine of representative strains (type strain not included) of the genus is 1,3-diaminopropane, spermidine is present in moderate amounts and putrescine and spermine are detectable only in minor amounts. The major fatty acids of strain Michel A/76(T) are C(14 : 0), C(16 : 0), C(16:1)omega7c and summed feature C(14 : 0) 3-OH/iso-C(16 : 1) I. This fatty acid profile is typical for members of the family Pasteurellaceae. The G+C content of DNA of strain Michel A/76(T) was estimated to be 52.5 mol% in a previous investigation. The type strain is P709(T) ( = Michel A/76(T) = CCUG 28028(T) = CIP 110147(T) = CCM 7802(T)).

Whole-exome sequencing reveals a C-terminal germline variant in CEBPA-associated acute myeloid leukemia: 45-year follow-up of a large family.

Familial acute myeloid leukemia is rare and linked to germline mutations in RUNX1, GATA2 or CCAAT/enhancer binding protein-α (CEBPA). We re-evaluated a large family with acute myeloid leukemia originally seen at NIH in 1969. We utilized whole-exome sequencing to study this family, and conducted in silico bioinformatics analysis, protein structural modeling and laboratory experiments to assess the impact of the identified CEBPA Q311P mutation. Unlike most previously identified germline mutations in CEBPA, which were N-terminal frameshift mutations, we identified a novel Q311P variant that was located in the C-terminal bZip domain of C/EBPα. Protein structural modeling suggested that the Q311P mutation alters the ability of the CEBPA dimer to bind DNA. Electrophoretic mobility shift assays showed that the Q311P mutant had attenuated binding to DNA, as predicted by the protein modeling. Consistent with these findings, we found that the Q311P mutation has reduced transactivation, consistent with a loss-of-function mutation. From 45 years of follow-up, we observed incomplete penetrance (46%) of CEBPA Q311P. This study of a large multi-generational pedigree reveals that a germline mutation in the C-terminal bZip domain can alter the ability of C/EBP-α to bind DNA and reduces transactivation, leading to acute myeloid leukemia.

Letter from Charles Stewart to his brother-in-law, William Tudor,

This letter was written aboard the U.S.S. Franklin. Stewart writes in detail about William’s brother Henry James (Harry) Tudor, and concerns about his character, particularly his "natural indolence and indifference." He notes that like his wife, Delia, Harry spends money irresponsibly. Stewart also writes he tried to interest Harry in the pursership of the Franklin, and had hoped he would be appointed to the Consulate of Tripoli or Tunis.

Letters from William Tudor to his sister, Delia Tudor Stewart,

Three letters written during Tudor’s various travels in England and the United States. In one letter, he writes of the precautions Delia should take in their correspondence in light of increasing tension between the United States and England. One letter is in French.

Letters from Delia Tudor Stewart to her brother, William Tudor,

Three letters written while Delia was traveling with her husband in South America aboard the U.S.S Franklin. In one letter, she writes about people she has met, including Condy Raguet, the United States consul at Rio de Janeiro.

Letter from A. Dickason to Delia Tudor Stewart,

Letter from an A. Dickason in London addressing Delia’s health and spirits. Also included are notes to Delia and her sister Emma from both "A.D." and "M.D."

Letter from John H. Ladd to Delia Tudor Stewart,

Letter to Delia in the care of her brother, William Tudor, in Rio de Janeiro.

Letter from Delia Jarvis Tudor to her daughter, Delia Tudor Stewart,

One letter regarding finances and news of friends, and asking her daughter when she plans to visit. One undated letter offering marital advice to Delia and discussing news regarding mutual acquaintances in South America, as well as the plans of her brother, Harry.

A family strike : a farce /

Mode of access: Internet.

The irresponsible five; a new family compact,

Mode of access: Internet.

[how The Gynecologist Can Guide Women With A Family History Of Cancer?].

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