The life of Robert Stephenson, F.R.S. etc. etc., late president of the Institution of Civil Engineers.

Mode of access: Internet.

Observations on the comparative merits of locomotive & fixed engines, as applied to railways : being a reply to the report of Mr. James Walker, to the directors of the Liverpool and Manchester Railway, compiled from the reports of Mr. George Stephenson ; With an account of the competition of locomotive engines at Rainhill, in October, 1829, and of the subsequent experiments /

"Account of the competition of locomotive engines ... at Rainhill in October, 1829," p. [60]-83.

Life and times of Sir Joshua Reynolds : with notices of some of his contemporaries ; commenced by Charles Robert Leslie ; continued and concluded by Tom Taylor.

Mode of access: Internet.

Risk analysis of prostate cancer in PRACTICAL, a multinational consortium, using 25 known prostate cancer susceptibility loci

Background Genome-wide association studies have identified multiple genetic variants associated with prostate cancer risk which explain a substantial proportion of familial relative risk. These variants can be used to stratify individuals by their risk of prostate cancer. Methods We genotyped 25 prostate cancer susceptibility loci in 40,414 individuals and derived a polygenic risk score (PRS).We estimated empirical odds ratios (OR) for prostate cancer associated with different risk strata defined by PRS and derived agespecific absolute risks of developing prostate cancer by PRS stratum and family history. Results The prostate cancer risk for men in the top 1% of the PRS distribution was 30.6 (95% CI, 16.4-57.3) fold compared with men in the bottom 1%, and 4.2 (95% CI, 3.2-5.5) fold compared with the median risk. The absolute risk of prostate cancer by age of 85 years was 65.8% for a man with family history in the top 1% of the PRS distribution, compared with 3.7% for a man in the bottom 1%. The PRS was only weakly correlated with serum PSA level (correlation = 0.09). Conclusions Risk profiling can identify men at substantially increased or reduced risk of prostate cancer. The effect size, measured by OR per unit PRS, was higher in men at younger ages and in men with family history of prostate cancer. Incorporating additional newly identified loci into a PRS should improve the predictive value of risk profiles. Impact:We demonstrate that the risk profiling based on SNPs can identify men at substantially increased or reduced risk that could have useful implications for targeted prevention and screening programs.

Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

Catalytic antibodies have shown great promise for catalyzing a tremendously diverse set of natural and unnatural chemical transformations. However, few catalytic antibodies have efficiencies that approach those of natural enzymes. In principle, random mutagenesis procedures such as phage display could be used to improve the catalytic activities of existing antibodies; however, these studies have been hampered by difficulties in the recombinant expression of antibodies. Here, we have grafted the antigen binding loops from a murine-derived catalytic antibody, 17E8, onto a human antibody framework in an effort to overcome difficulties associated with recombinant expression and phage display of this antibody. “Humanized” 17E8 retained similar catalytic and hapten binding properties as the murine antibody while levels of functional Fab displayed on phage were 200-fold higher than for a murine variable region/human constant region chimeric Fab. This construct was used to prepare combinatorial libraries. Affinity panning of these resulted in the selection of variants with 2- to 8-fold improvements in binding affinity for a phosphonate transition-state analog. Surprisingly, none of the affinity-matured variants was more catalytically active than the parent antibody and some were significantly less active. By contrast, a weaker binding variant was identified with 2-fold greater catalytic activity and incorporation of a single substitution (Tyr-100aH → Asn) from this variant into the parent antibody led to a 5-fold increase in catalytic efficiency. Thus, phage display methods can be readily used to optimize binding of catalytic antibodies to transition-state analogs, and when used in conjunction with limited screening for catalysis can identify variants with higher catalytic efficiencies.

Select speeches of the Right Honourable George Canning : with a preliminary biographical sketch, and an appendix, of extracts from his writings and speeches /

Mode of access: Internet.

Zur Geschichte des deutschen Zollvereins : zwei apokryphische Capitel und ein prophetisches /

Mode of access: Internet.

The works of the British poets : with lives of the authors /

Imprint varies : vols. 1-25: as above; vols. 26-29, 32-35, 43-45, 47-50: Philadelphia, Samuel F. Bradford for John Laval.

Life and times of Sir Joshua Reynolds : with notices of some of his contemporaries /

Includes index.

Museum.

Title from caption.

The clerk's guide, or, Commercial correspondence; comprising letters of business, forms of bills, invoices, account-sales, and an appendix, containing advice to young tradesmen and shopkeepers, equation of payments, commercial terms, &c.

With this is bound: Foster, B. F. Prospectus of the Commercial Academy, no. 183 Broadway, N. Y., conducted by B. F. Foster ... New York, 1837.

Works of the British poets, with lives of the authors; translations.

v. 1-2. The Iliad of Homer, tr. by Alexander Pope.--v. 3. The Odyssey of Homer, tr. by Alexander Pope.--v. 4-5. The comedies of Aristophanes; by T. Mitchell.--v. 5. The comedies of Aristophanes; Select comedies of Terence, tr. by George Coleman, The satires of Perseus, tr. into English verse by William Gifford.--v. 6-7. The vision of Dante Alighieri, tr. by H.F. Cary.--v. 8. The Lusiad; tr. fr. Camoens by W.J. Mickle.--v. 9-10. Tasso's Jerusalem delivered; tr. by J.H. Hunt.--v. 11. Selections from the tragedies of Aeschylus, Sophocles & Euripides.

A practical summary of the law and usage of bills of exchange and promissory notes [microform] : together with a series of tables, showing when bills, notes and drafts, drawn or accepted at any date, will fall due : to which are added rates of commisson and storage, equation of payments, and general information connected with the business of the counting-house /

Mode of access: Internet.

Zur Geschichte des deutschen Zollvereins : zwei apokryphische Capitel und ein Prophetisches /

Goldsmiths'-Kress no. 32168.29.

O soborakh, byvshikh v Rossīi so vremeni vvedenīi︠a︡ v neĭ khristi︠a︡nstva so t︠s︡arstvovanīi︠a︡ Ioanna IV Vasilʹevicha.

Bibliographical references included in "Primechanīi︠a︡".