20 resultados para Sodalis
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Franc. Philelfi orationes (34). - Philelfus Francisco Sforcie (92). - Guarinus Veron. Conrado Alcaldo (96). - De matrimonio inter Stephanum Salvanum et Margaritam Pontam (96 v°). - Plutarchus, de liberis educandis, interprete Garino Veron. (101).
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Data sheet produced by the Iowa Department of Natural Resources is about different times of animals, insects, snakes, birds, fish, butterflies, etc. that can be found in Iowa.
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Franc. Philelfi orationes (34). - Philelfus Francisco Sforcie (92). - Guarinus Veron. Conrado Alcaldo (96). - De matrimonio inter Stephanum Salvanum et Margaritam Pontam (96 v°). - Plutarchus, de liberis educandis, interprete Garino Veron. (101).
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Sodalis glossinidius is a maternally transmitted secondary endosymbiont residing intracellularly in tissues of the tsetse flies, Glossina spp. In this study, we have used Tn5 mutagenesis and a negative selection procedure to derive a S. glossinidius mutant that is incapable of invading insect cells in vitro and is aposymbiotic when microinjected into tsetse. This mutant strain harbors Tn5 integrated into a chromosomal gene sharing high sequence identity with a type III secretion system invasion gene (invC) previously identified in Salmonella enterica. With the use of degenerate PCR, we have amplified a further six Sodalis inv/spa genes sharing high sequence identity with type III secretion system genes encoded by Salmonella pathogenicity island 1. Phylogenetic reconstructions based on the inv/spa genes of Sodalis and other members of the family Enterobacteriaceae have consistently identified a well-supported clade containing Sodalis and the enteric pathogens Shigella and Salmonella. These results suggest that Sodalis may have evolved from an ancestor with a parasitic intracellular lifestyle, possibly a latter-day entomopathogen. These observations lend credence to a hypothesis suggesting that vertically transmitted mutualistic endosymbionts evolve from horizontally transmitted parasites through a parasitism–mutualism continuum.
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"Final report: project E-3."
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Tomus III has imprint: In bibliopolio Gleditschiano.
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Trypanosomiasis has been identified as a neglected tropical disease in both humans and animals in many regions of sub-Saharan Africa. Whilst assessments of the biology of trypanosomes, vectors, vertebrate hosts and the environment have provided useful information about life cycles, transmission, and pathogenesis of the parasites that could be used for treatment and control, less information is available about the effects of interactions among multiple intrinsic factors on trypanosome presence in tsetse flies from different sites. It is known that multiple species of tsetse flies can transmit trypanosomes but differences in their vector competence has normally been studied in relation to individual factors in isolation, such as: intrinsic factors of the flies (e.g. age, sex); habitat characteristics; presence of endosymbionts (e.g. Wigglesworthia glossinidia, Sodalis glossinidius); feeding pattern; host communities that the flies feed on; and which species of trypanosomes are transmitted. The purpose of this study was to take a more integrated approach to investigate trypanosome prevalence in tsetse flies. In chapter 2, techniques were optimised for using the Polymerase Chain Reaction (PCR) to identify species of trypanosomes (Trypanosoma vivax, T. congolense, T. brucei, T. simiae, and T. godfreyi) present in four species of tsetse flies (Glossina austeni, G. brevipalpis, G. longipennis and G. pallidipes) from two regions of eastern Kenya (the Shimba Hills and Nguruman). Based on universal primers targeting the internal transcribed spacer 1 region (ITS-1), T. vivax was the predominant pathogenic species detected in flies, both singly and in combination with other species of trypanosomes. Using Generalised Linear Models (GLMs) and likelihood ratio tests to choose the best-fitting models, presence of T. vivax was significantly associated with an interaction between subpopulation (a combination between collection sites and species of Glossina) and sex of the flies (X2 = 7.52, df = 21, P-value = 0.0061); prevalence in females overall was higher than in males but this was not consistent across subpopulations. Similarly, T. congolense was significantly associated only with subpopulation (X2 = 18.77, df = 1, P-value = 0.0046); prevalence was higher overall in the Shimba Hills than in Nguruman but this pattern varied by species of tsetse fly. When associations were analysed in individual species of tsetse flies, there were no consistent associations between trypanosome prevalence and any single factor (site, sex, age) and different combinations of interactions were found to be significant for each. The results thus demonstrated complex interactions between vectors and trypanosome prevalence related to both the distribution and intrinsic factors of tsetse flies. The potential influence of the presence of S. glossinidius on trypanosome presence in tsetse flies was studied in chapter 3. A high number of Sodalis positive flies was found in the Shimba Hills, while there were only two positive flies from Nguruman. Presence or absence of Sodalis was significantly associated with subpopulation while trypanosome presence showed a significant association with age (X2 = 4.65, df = 14, P-value = 0.0310) and an interaction between subpopulation and sex (X2 = 18.94, df = 10, P-value = 0.0043). However, the specific associations that were significant varied across species of trypanosomes, with T. congolense and T. brucei but not T. vivax showing significant interactions involving Sodalis. Although it has previously been concluded that presence of Sodalis increases susceptibility to trypanosomes, the results presented here suggest a more complicated relationship, which may be biased by differences in the distribution and intrinsic factors of tsetse flies, as well as which trypanosome species are considered. In chapter 4 trypanosome status was studied in relation to blood meal sources, feeding status and feeding patterns of G. pallidipes (which was the predominant fly species collected for this study) as determined by sequencing the mitochondrial cytochrome B gene using DNA extracted from abdomen samples. African buffalo and African elephants were the main sources of blood meals but antelopes, warthogs, humans, giraffes and hyenas were also identified. Feeding on multiple hosts was common in flies sampled from the Shimba Hills but most flies from Nguruman had fed on single host species. Based on Multiple Correspondence Analysis (MCA), host-feeding patterns showed a correlation with site of sample collection and Sodalis status, while trypanosome status was correlated with sex and age of the flies, suggesting that recent host-feeding patterns from blood meal analysis cannot predict trypanosome status. In conclusion, the complexity of interactions found suggests that strategies of tsetse fly control should be specific to particular epidemic areas. Future studies should include laboratory experiments that use local colonies of tsetse flies, local strains of trypanosomes and local S. glossinidius under controlled environmental conditions to tease out the factors that affect vector competence and the relative influence of external environmental factors on the dynamics of these interactions.
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The extensive antigenic variation phenomena African trypanosomes display in their mammalian host have hampered efforts to develop effective vaccines against trypanosomiasis. Human disease management aims largely to treat infected hosts by chemotherapy, whereas control of animal diseases relies on reducing tsetse populations as well as on drug therapy. The control strategies for animal diseases are carried out and financed by livestock owners, who have an obvious economic incentive. Sustaining largely insecticide-based control at a local level and relying on drugs for treatment of infected hosts for a disease for which there is no evidence of acquired immunity could prove extremely costly in the long run. It is more likely that a combination of several methods in an integrated, phased and area-wide approach would be more effective in controlling these diseases and subsequently improving agricultural output. New approaches that are environmentally acceptable, efficacious and affordable are clearly desirable for control of various medically and agriculturally important insects including tsetse. Here, Serap Aksoy and colleagues discuss molecular genetic approaches to modulate tsetse vector competence.
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Contient : 1° Extrait du « Livre du Regime des Princes », de « GILLE DE ROMME », traduction [de JEAN GOLEIN] ; 2° « Livre de Viellesse », de « TULLE », traduction de « LAURENT » [DE PREMIERFAIT] ; 3° « Livre de la vraie Amitié », de « TULLE », traduction de « LAURENT DE PREMIERFAIT » ; 4° « Quadrilogium invectivum » [d'ALAIN CHARTIER] ; 5° « Dialogus familiaris amici et sodalis super depploracionem galice calamitatis, ab ALANO AURIGE editus » ; 6° Le Curial [d'ALAIN CHARTIER]
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Contient : 1° Extrait du « Livre du Regime des Princes », de « GILLE DE ROMME », traduction [de JEAN GOLEIN] ; 2° « Livre de Viellesse », de « TULLE », traduction de « LAURENT » [DE PREMIERFAIT] ; 3° « Livre de la vraie Amitié », de « TULLE », traduction de « LAURENT DE PREMIERFAIT » ; 4° « Quadrilogium invectivum » [d'ALAIN CHARTIER] ; 5° « Dialogus familiaris amici et sodalis super depploracionem galice calamitatis, ab ALANO AURIGE editus » ; 6° Le Curial [d'ALAIN CHARTIER]
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Contient : 1° « Quadrilogum invectivum et comicum ad morum Gallicorum correctionem », par « ALLAIN CHARTIER » ; 2° « Dialogus familiaris amici et sodalis super deploracione galice calamitatis, ab ALANO AURIGE editus » ; 3° L'Espérance, ou Consolation des trois vertus [par ALAIN CHARTIER]
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Contient : 1° Le Quadrilogue invectif, par « ALAIN CHARRETIER » ; 2° « Dialogus familiaris amici et sodalis super deploracione gallice calamitatis, ab ALANO AURIGE editus » ; 3° « L'Esperance, ou Consolation des trois vertus, Foy, Esperance, Charité » [par ALAIN CHARTIER]
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Contient : 1° « Le Quadrilogue invectif », par « ALAIN CHARETIER » ; 2° « Dyalogus familiaris amici et sodalis super deploratione galice calamitatis, ab ALANO AURIGE editus » ; 3° L'Espérance, ou Consolation des trois vertus ; 4° Le Dit « de paix eureuse, par maistre ALLAIN CHARETIER » ; 5° Le Débat des deux fortunes d'amour, par « ALLAIN » ; 6° Épître aux trois États sur les malheurs du royaume en 1435