Sca-1+ progenitors derived from embryonic stem cells differentiate into endothelial cells capable of vascular repair after arterial injury

Embryonic stem cells possess the ability to differentiate into endothelium. The ability to produce large volumes of endothelium from embryonic stem cells could provide a potential therapeutic modality for vascular injury. We describe an approach that selects endothelial cells using magnetic beads that may be used therapeutically to treat arterial injury.

Regional report on measuring disability: Overview of the disability measurement procedures in Latin America and the Caribbean. Task Force on Disability Measurement Statistical Conference of the Americas (SCA)

Introduction .-- I. Inclusion of international guidelines in disability measurement processes in Latin American and Caribbean countries .-- II. State of the art in disability measurement in the region’s countries .-- III. Main difficulties facing countries in terms of collecting information .-- Conclusions.

Identification of small Sca-1(+), Lin(-), CD45(-) multipotential cells in the neonatal murine retina

OBJECTIVE: Bone marrow contains a subset of stem cells that give rise to nonhematopoietic lineages. These nonhematopoietic stem cells appear heterogeneous and contain cells committed to mesenchymal and endothelial lineages, as well as more primitive multipotential cells resembling progenitors of germ cells and very small embryonic/epiblast-like stem cells (VSELs). Nonhematopoietic stem cells can be mobilized from the bone marrow in response to tissue injury, and cells with similar properties have been found in cord blood and normal adult organs. However, the relationship between bone marrow cells and these adult organ stem cells is still unclear. The differentiation potential of some adult stem cells is organ-restricted, but other populations appear to retain multipotential capacity. MATERIALS AND METHODS: A population of small Sca-1(+), lineage-negative (Lin(-)), CD45(-) cells resembling VSELs were isolated from neonatal mouse retina by cell sorting. Differentiation of the cells in culture was achieved by exposure to embryonic stem cell differentiation protocols. RESULTS: VSEL-like cells comprise 1.5% of the neonatal mouse retina. They remain quiescent during retinal differentiation, and thus they do not contribute to normal retinal development. However, they display eye cell differentiation potential in culture and they are also multipotential and can give rise to cells representative of all three embryonic layers. CONCLUSIONS: The neonatal retina is an abundant postnatal source of multipotential VSEL-like cells that can differentiate in culture into a variety of lineages.

SCA security verification on wireless sensor network node

Side Channel Attack (SCA) differs from traditional mathematic attacks. It gets around of the exhaustive mathematic calculation and precisely pin to certain points in the cryptographic algorithm to reveal confidential information from the running crypto-devices. Since the introduction of SCA by Paul Kocher et al [1], it has been considered to be one of the most critical threats to the resource restricted but security demanding applications, such as wireless sensor networks. In this paper, we focus our work on the SCA-concerned security verification on WSN (wireless sensor network). A detailed setup of the platform and an analysis of the results of DPA (power attack) and EMA (electromagnetic attack) is presented. The setup follows the way of low-cost setup to make effective SCAs. Meanwhile, surveying the weaknesses of WSNs in resisting SCA attacks, especially for the EM attack. Finally, SCA-Prevention suggestions based on Differential Security Strategy for the FPGA hardware implementation in WSN will be given, helping to get an improved compromise between security and cost.

Conversacio entre Sento, Tito y Quelo de Payporta [Texto impreso] : pòsense à rahonar Cènto, Tito y Quèlo de Payporta, al eixir de Misa un dumenge, à la sóca de un marche allí chitats

Hay un ejemplar encuadernado con: Poesías colocadas en el pórtico del Convento de San Francisco de Valencia (NP849.91/3086).

Questions and answers relating to AWS and SCA survey job descriptions.

"January 1991."

Um projecto de educação para a saúde na SCA

As Síndromes Coronárias Agudas (SCA) são responsáveis por elevadas taxas de mortalidade em todo o mundo. Segundo o Ministério da Saúde, “as doenças cardiovasculares (…) são a principal causa de mortalidade em Portugal, tal como se verifica em muitos países ocidentais, sendo considerada, no entanto, das mais elevadas da Europa e do Mundo” (MINISTÉRIO DA SAÚDE, 2006, p. 2). As intervenções de enfermagem na fase aguda das SCA são fulcrais e consistem na atenção dirigida à proteção e promoção da vida com base nas suas competências específicas, alívio da dor e desenvolvimento de uma relação terapêutica que passa pela consciencialização da necessidade de alterações dos hábitos não saudáveis de vida entre as pessoas internadas. Estas intervenções devem iniciar-se precocemente através de um processo de identificação e reconhecimento da necessidade de alterações comportamentais, educação para a saúde e planeamento do regime terapêutico, favorecendo a adesão a programas de reabilitação cardíaca. Verificamos que muitas das admissões no serviço de cardiologia, por SCA, são reinternamentos, sugerindo que a vigilância à saúde pode não ser a mais adequada. Neste trabalho pretende-se a partilha da experiência de educação para a saúde à pessoa com SCA no serviço de cardiologia de um hospital da região metropolitana de Lisboa. O serviço de cardiologia do HFF tem desenvolvido desde o ano 2000 um programa de educação para a saúde nas pessoas com SCA com extensão às suas famílias. Consistia, inicialmente, numa intervenção iniciada à cabeceira do doente como processo natural de educação para a saúde e, por ocasião da passagem pela enfermaria, com a apresentação de uma sessão em PowerPoint e disponibilização de um manual em formato papel. Ao longo dos anos o programa vem sofrendo modificações para melhor adequação às necessidades de educação para a saúde do indivíduo. Refletimos acerca da pertinência destas intervenções, o seu impacto sobre a saúde das pessoas e a relevância em termos de alteração de hábitos não saudáveis de vida entre os doentes internados. Neste processo de reformulação do programa, analisamos os processos hospitalares dos doentes submetidos às sessões de educação para a saúde num intervalo de 6 meses (janeiro a junho de 2014) e efetuamos entrevistas telefónicas a esta população um ano após a data do internamento. Dos dados analisados percebemos que uma parcela significativa destas pessoas não se lembrava da sessão de educação para a saúde. Por outro lado, das que se recordavam, revelaram o relevante impacto da educação para a saúde na alteração de hábitos não saudáveis de vida. A análise dos dados permitiu a reorganização das sessões de educação para a saúde desde o seu conteúdo até à metodologia. Ainda muitos passos devem ser dados no aperfeiçoamento deste projecto. Pretendemos elaborar um protocolo que permita uniformizar a prática de educação para a saúde e introduzir instrumentos de avaliação em cada etapa do mesmo. BIBLIOGRAFIA Cossette, S., D´Aoust, L.-X., Morin, M., Heppell, S., & Frasure-Smith, N. (2009). The Systematic Development of a Nursing Intervention Aimed at Increasing Enrollment in Cardiac Rehabilitation for Acute Coronary Syndrome Patients. Progress in Cardiovascular Nursing, 24, pp. 71-79. Fernandez, R., Davidson, P., Griffiths, R., Juergens, C., & Salamonson, Y. (2007). What do we know about the long term medication adherence in patients following percutaneous coronary intervention? Australian Journal of Advanced Nursing, 25 (2), pp. 53-61. Ministério da Saúde. (2006). Programa Nacional de Prevenção e Controlo das Doenças Cardiovasculares. Lisboa. Mota, T. G., Clara, J. G., Gonçalves, J. V., Rocha, A. P., Neves, A. P., & Santos, T. M. (2003). Passaporte para a Vida. Coimbra: Grupo de Estudos de Hemodinâmica e Cardiologia de Intervenção da Sociedade Portuguesa de Cardiologia. Ordem dos Enfermeiros. (2003). Competências do enfermeiro de cuidados gerais. Lisboa. Santos, L. S., & Henriques, E. (2012). Gestão do regime terapêutico no pós- EAM: desenvolvimento de um protocolo de intervenção de enfermagem em follow-up. Relatório de Estágio de Mestrado, Escola Superior de Enfermagem de Lisboa, Lisboa. Thelan, L. A., Davie, J. K., Urden, L. D., & Lough, M. E. (1996). Enfermagem em Cuidados Intensivos ̶ Diagnóstico e Intervenção. Lisboa: Lusodidacta. Urden, L. D., Stacy, K. M., & Lough, M. E. (2008). Thelan´s Enfermagem de Cuidados Intensivos ̶ Diagnóstico e Intervenção. Loures: Lusodidacta.

The molecular mechanisms utilised by mesenchymal stem cells in migration to acute myocardial infarction

Heart damage caused by acute myocardial infarction (AMI) is a leading cause of death and disability in Australia. Novel therapies are still required for the treatment of this condition due to the poor reparative ability of the heart. As such, cellular therapies that assist in the recovery of heart muscle are of great current interest. Culture expanded mesenchymal stem cells (MSC) represent a stem and progenitor cell population that has been shown to promote tissue recovery in pre-clinical studies of AMI. For MSC-based therapies in the clinic, an intravenous route of administration would ideally be used due to the low cost, ease of delivery and relative safety. The study of MSC migration is therefore clinically relevant for a minimally invasive cell therapy to promote regeneration of damaged tissue. C57BL/6, UBI-GFP-BL/6 and CD44-/-/GFP+/+ mice were utilised to investigate mMSC migration. To assist in murine models of MSC migration, a novel method was used for the isolation of murine MSC (mMSC). These mMSC were then expanded in culture and putative mMSC were positive for Sca-1, CD90.2, and CD44 and were negative for CD45 and CD11b. Furthermore, mMSC from C57BL/6 and UBI-GFP-BL/6 mice were shown to differentiate into cells of the mesodermal lineage. Cells from CD44-/-/GFP+/+ mice were positive for Sca-1 and CD90.2, and negative for CD44, CD45 and CD11b however, these cells were unable to differentiate into adipocytes and chondrocytes and express lineage specific genes, PLIN and ACAN. Analysis of mMSC chemokine receptor (CR) expression showed that although mMSC do express chemokine receptors, (including those specific for chemokines released after AMI), these were low or undetectable by mRNA. However, protein expression could be detected, which was predominantly cytoplasmic. It was further shown that in both healthy (unperturbed) and inflamed tissues, mMSC had very little specific migration and engraftment after intravenous injection. To determine if poor mMSC migration was due to the inability of mMSC to respond to chemotactic stimuli, chemokine expression in bone marrow, skin injury and hearts (healthy and after AMI) was analysed at various time points by quantitative real-time PCR (qRT PCR). Many chemokines were up-regulated after skin biopsy and AMI, but the highest acute levels were found for CXCL12 and CCL7. Due to their high expression in infarcted hearts, the chemokines CXCL12 and CCL7 were tested for their effect on mMSC migration. Despite CR expression at both protein and mRNA levels, migration in response to CXCL12 and CCL7 was low in mMSC cultured on Nunclon plastic. A novel tissue culture plastic technology (UpCellTM) was then used that allowed gentle non-enzymatic dissociation of mMSC, thus preserving surface expression of the CRs. Despite this the in vitro data indicated that CXCL12 fails to induce significant migration ability of mMSC, while CCL7 induces significant, but low-level migration. We speculated this may be because of low levels of surface expression of chemokine receptors. In a strategy to increase cell surface expression of mMSC chemokine receptors and enhance their in vitro and in vivo migration capacity, mMSC were pre-treated with pro-inflammatory cytokines. Increased levels of both mRNA and surface protein expression were found for CRs by pre-treating mMSC with pro-inflammatory cytokines including TNF-á, IFN-ã, IL-1á and IL-6. Furthermore, the chemotactic response of mMSC to CXCL12 and CCL7 was significantly higher with these pretreated cells. Finally, the effectiveness of this type of cell manipulation was demonstrated in vivo, where mMSC pre-treated with TNF-á and IFN-ã showed significantly increased migration in skin injury and AMI models. Therefore this thesis has demonstrated, using in vitro and in vivo models, the potential for prior manipulation of MSC as a possible means for increasing the utility of intravenously delivery for MSC-based cellular therapies.

Toward resolving deep neoaves phylogeny : data, signal enhancement, and priors

We report three developments toward resolving the challenge of the apparent basal polytomy of neoavian birds. First, we describe improved conditional down-weighting techniques to reduce noise relative to signal for deeper divergences and find increased agreement between data sets. Second, we present formulae for calculating the probabilities of finding predefined groupings in the optimal tree. Finally, we report a significant increase in data: nine new mitochondrial (mt) genomes (the dollarbird, New Zealand kingfisher, great potoo, Australian owlet-nightjar, white-tailed trogon, barn owl, a roadrunner [a ground cuckoo], New Zealand long-tailed cuckoo, and the peach-faced lovebird) and together they provide data for each of the six main groups of Neoaves proposed by Cracraft J (2001). We use his six main groups of modern birds as priors for evaluation of results. These include passerines, cuckoos, parrots, and three other groups termed “WoodKing” (woodpeckers/rollers/kingfishers), “SCA” (owls/potoos/owlet-nightjars/hummingbirds/swifts), and “Conglomerati.” In general, the support is highly significant with just two exceptions, the owls move from the “SCA” group to the raptors, particularly accipitrids (buzzards/eagles) and the osprey, and the shorebirds may be an independent group from the rest of the “Conglomerati”. Molecular dating mt genomes support a major diversification of at least 12 neoavian lineages in the Late Cretaceous. Our results form a basis for further testing with both nuclear-coding sequences and rare genomic changes.

Competing through service innovation : the role of bricolage and entrepreneurship in project-oriented firms

The criticality of service innovation in building and sustaining competitive advantage is gaining increasing recognition in the marketplace. Using empirical data from US and Australian project-oriented firms, the study uses a multi-staged multi-method research program to demonstrate how entrepreneurial service firms strategically combine resources at hand (bricolage) to innovate and stay ahead of rivals. The research shows that service entrepreneurship (SE) and bricolage influence two forms of service innovation (interactive and supportive), which in turn is associated with sustained competitive advantage (SCA). The results suggest that SE and bricolage indirectly relate to SCA through service innovation. The findings offer novel insights into how project-oriented service firms engage in innovation. In short, the findings encourage the “making do by combining resources at hand” as higher levels of entrepreneurial bricolage are associated with higher levels of interactive and supportive innovation enabling SCA, suggesting a new model.

Post-genomic science & society : Getting our fangs into omics science and its implications

Genomics and genetic findings have been hailed with promises of unlocked codes and new frontiers of personalized medicine. Despite cautions about gene hype, the strong cultural pull of genes and genomics has allowed consideration of genomic personhood. Populated by the complicated records of mass spectrometer, proteomics, which studies the human protein, has not achieved either the funding or the popular cultural appeal proteomics scientists had hoped it would. While proteomics, being focused on the proteins that actually indicate and create disease states, has a more direct potential for clinical applications than genomic risk predictions, culturally, it has not provided the material for identity creation. In our ethnographic research, we explore how proteomic scientists attempting to shape an appeal to personhood through which legitimacy may be defined.

Engraftment outcomes after HPC co-culture with mesenchymal stromal cells and osteoblasts

Haematopoietic stem cell (HSC) transplantation is an established cell-based therapy for a number of haematological diseases. To enhance this therapy, there is considerable interest in expanding HSCs in artificial niches prior to transplantation. This study compared murine HSC expansion supported through co-culture on monolayers of either undifferentiated mesenchymal stromal cells (MSCs) or osteoblasts. Sorted Lineage− Sca-1+ c-kit+ (LSK) haematopoietic stem/progenitor cells (HPC) demonstrated proliferative capacity on both stromal monolayers with the greatest expansion of LSK shown in cultures supported by osteoblast monolayers. After transplantation, both types of bulk-expanded cultures were capable of engrafting and repopulating lethally irradiated primary and secondary murine recipients. LSKs co-cultured on MSCs showed comparable, but not superior, reconstitution ability to that of freshly isolated LSKs. Surprisingly, however, osteoblast co-cultured LSKs showed significantly poorer haematopoietic reconstitution compared to LSKs co-cultured on MSCs, likely due to a delay in short-term reconstitution. We demonstrated that stromal monolayers can be used to maintain, but not expand, functional HSCs without a need for additional haematopoietic growth factors. We also demonstrated that despite apparently superior in vitro performance, co-injection of bulk cultures of osteoblasts and LSKs in vivo was detrimental to recipient survival and should be avoided in translation to clinical practice.

Second-harmonic generation from biological tissues : effect of excitation wavelength

We report on the measurement of second-harmonic signals from hyperplastic parenchyma and stroma in malignant human prostate tissue under femtosecond pulsed illumination in the wavelength range from 730 to 870 nm. In particular, the relationship of the second-harmonic generation to the excitation wavelength is measured. The result in these two regions behaves considerably differently and thus provides a possible indicator for identifying tissue components and malignancy.

Corporate governance and securities class actions

This study investigates the governance attributes of firms that have been subject to securities class actions (SCAs). There has been a recent sizable increase in the number of firms subject to SCAs in Australia. We examine a sample of firms that have been subject to SCAs due to disclosure breaches and match the firms by industry and size to a control sample. First, we examine the compliance culture of the SCA firms via the frequency of Australian Securities Exchange (ASX)queries of the firm and find that the frequency of ASX queries is positively associated with the occurrence of a SCA. Secondly, we provide evidence that SCA firms exhibit weaker levels of corporate governance than the matched control sample. In addition, we contribute to the understanding of firms subject to SCAs and their corporate governance attributes. Our results suggest the presence of a nomination committee may be associated with higher agency costs and that the influence of CEO duality may reduce the effectiveness of a nomination committee.

Side Channel Analysis of Some Hash Based MACs: A Response to SHA-3 Requirements

The forthcoming NIST’s Advanced Hash Standard (AHS) competition to select SHA-3 hash function requires that each candidate hash function submission must have at least one construction to support FIPS 198 HMAC application. As part of its evaluation, NIST is aiming to select either a candidate hash function which is more resistant to known side channel attacks (SCA) when plugged into HMAC, or that has an alternative MAC mode which is more resistant to known SCA than the other submitted alternatives. In response to this, we perform differential power analysis (DPA) on the possible smart card implementations of some of the recently proposed MAC alternatives to NMAC (a fully analyzed variant of HMAC) and HMAC algorithms and NMAC/HMAC versions of some recently proposed hash and compression function modes. We show that the recently proposed BNMAC and KMDP MAC schemes are even weaker than NMAC/HMAC against the DPA attacks, whereas multi-lane NMAC, EMD MAC and the keyed wide-pipe hash have similar security to NMAC against the DPA attacks. Our DPA attacks do not work on the NMAC setting of MDC-2, Grindahl and MAME compression functions.