Rooster's off to see the world.

Una buena mañana, un gallo decidió que quería viajar. Por el camino se le unieron dos gatos, tres ranas, cuatro tortugas y cinco peces, todos deseosos de conocer el mundo. Pero descubren que llevar una vida itinerante es un trabajo más difícil de lo que pensaban. Esta historia sirve para iniciar a los niños en el significado de los números y en las operaciones aritméticas de suma y resta.

Behaviour of the germ cell specific lamin through mammalian spermatogenesis as probed with monoclonal antibodies.

We had earlier identified a 60 kDa nuclear lamin protein (lamin(g)) unique to the germ cells of rat testis which was subsequently shown to be antigenically conserved in germ cells of grasshopper, rooster, frog and plants. We have now obtained eight monoclonal antibodies in mouse against this lamin(g) antigen. While all the eight Mabs reacted with lamin(g) antigen in an immunoblot analysis, only three Mabs (A(11)C(7), A(11)D(4), C1F7) showed strong reactivity in the immunofluorescence analysis of the germ cells. The Mabs A(11)C(7) and A(11)D(4) showed a slight cross-reactivity with rat liver lamin B. Indirect immunofluorescence analysis of pre-meiotic, meiotic and post-meiotic germ cells with Mabs have shown that while the lamin(g) is localized in the lamina structures of spermatogonia and round spermatids, it is localized to the phase dense regions of pachytene spermatocytes which is in conformity with our previous observations using rabbit polyclonal antibodies. The localization of the antigen in the germ cells was also confirmed by immunohistochemical staining of the thin sections of seminiferous tubules. By immunostaining the surface spread pachytene spermatocytes, the antigen was further localized to the telomeric ends of the paired homologous chromosomes. Using anti-somatic lamin B antibodies, we have also demonstrated the absence of somatic lamins in meiotic and post-meiotic germ cells. The lamina structure of pre-meiotic spermatogonial nucleus contains both somatic lamin B and lamin(g) as evidenced by immunofluorescence studies with two differently fluorochrome labelled anti-lamin B and anti-lamin(g) antibodies. The selective retention of lamin(g) in the pachytene spermatocytes is probably essential for anchoring the telomeric ends of the paired chromosomes to the inner nuclear membrane.

The effects of anesthesia with a combination of intramuscular xylazine-diazepam-ketamine on heart rate, respiratory rate and cloacal temperature in roosters

Objective To examine the anesthetic effects of a xylazine-diazepam-ketamine (XDK) combination in roosters.Study design Prospective experimental trial.Animals Six healthy white Leghorn roosters weighing 2.03 +/- 0.08 kg.Methods Each rooster was pre-medicated with xylazine (3 mg kg(-1), IM) and after 15 minutes anesthesia was induced with a diazepam (4 mg kg(-1)) and ketamine (25 mg kg(-1)) combination injected into the pectoral muscles. Heart and respiratory rates were recorded before anesthesia and every 15 minutes after induction for 165 minutes. Cloacal temperature was measured before and 15 minutes after pre-medication and every 75 minutes thereafter during anesthesia. Quality of induction and recovery were scored subjectively; duration of loss of righting reflex, abolition of response to a painful stimulus and palpebral reflex were also recorded.Results Intramuscular injection of xylazine smoothly induced loss of the righting reflex within 3-4 minutes. Loss of response to a painful stimulus occurred at 13.1 +/- 2.9 minutes (mean +/- SD) after the administration of the D-K combination, and lasted for 63.0 +/- 5.3 minutes. Roosters anesthetized with this combination had a significant decrease in heart and respiratory rates and cloacal temperature. The recovery period lasted for up to 4 hours (227.5 +/- 15.4 minutes). Quality of recovery was satisfactory for four roosters but excitation was noted in two birds.Conclusions and clinical relevance The XDK combination was a useful anesthetic technique for typhlectomy in roosters. Nevertheless this drug combination should be used with caution and cardiopulmonary parameters monitored carefully. Under the conditions of this experiment it was associated with a decreased cloacal temperature and prolonged recoveries.

Determinação do Valor Nutricional das Sojas Integrais Processadas para Aves

Três ensaios biológicos foram realizados com o objetivo de determinar o conteúdo de energia metabolizável e do coeficientes de digestibilidade do extrato etéreo da soja integral processada por diferentes métodos (extrusão e tostagem) e do farelo de soja reconstituído com óleo. No primeiro e segundo ensaios, foram utilizadas a metodologia tradicional de coleta total de excretas, utilizando pintos no primeiro ensaio e galos no segundo. No terceiro ensaio, empregou-se a metodologia de alimentação forçada (Sibbald, 1976) com galos adultos. de acordo com os resultados obtidos, as sojas testadas apresentaram composições bromatológicas semelhantes. Os processamentos usados para a soja integral conferiram diferenças na digestibilidade dos nutrientes da soja e os coeficientes de digestibilidade do extrato etéreo obtidos para a soja extrusada e para o farelo de soja com adição de óleo foram superiores aos obtidos para soja tostada pelo vapor. Os valores de energia metabolizável obtidos para a soja extrusada foram superiores aos encontrados para a soja tostada pelo vapor e para o farelo de soja com adição de óleo, que foram semelhantes entre si.

Composição e Digestibilidade dos Aminoácidos das Sojas Integrais Processadas para Aves

O presente trabalho foi realizado com o objetivo de determinar a composição em aminoácidos e os coeficientes de digestibilidade dos aminoácidos da soja integral extrusada, tostada e do farelo de soja reconstituído de óleo. Foi utilizada a metodologia da alimentação forçada (Sibbad, 1976) com galos adultos. Para o cálculo dos coeficientes de digestibilidade dos aminoácidos das sojas testadas, foram consideradas as perdas endógenas e metabólicas obtidas de galos em jejum. Os dados médios de digestibilidade verdadeira de todos os aminoácidos testados foram de 91,1% para a soja extrusada, 78,6% para a soja tostada e 90,5% para o farelo de soja. Os resultados obtidos permitem concluir que a digestibilidade dos aminoácidos da soja integral tostada foi inferior a da soja extrusada e farelo de soja + óleo, que por sua vez foram semelhantes entre si. Portanto, pode-se afirmar que os diferentes tipos de processamentos da soja integral conferem a esse alimento características nutricionais distintas para aves.

Efeito do processamento da soja integral sobre a energia metabolizável e a digestibilidade dos aminoácidos para aves

Essa pesquisa foi desenvolvida para se avaliar o valor nutricional da soja integral submetida a diferentes processamentos. Quatro ensaios de metabolismo foram conduzidos para determinar os coeficientes de digestibilidade da matéria seca (CDMS), do extrato etéreo (CDEE) e dos aminoácidos e os valores de energia metabolizável aparente e verdadeira da soja integral desativada (SID) e extrusada (SIE) e das misturas de farelo de soja com óleo degomado de soja (FSO) ou com óleo ácido de soja (FSOA). Nos ensaios um e dois, utilizou-se a metodologia tradicional de coleta total de excretas com pintos e galos, respectivamente. A metodologia da alimentação forçada com galos adultos intactos foi utilizada no ensaio três, e com galos cecotomizados, no ensaio quatro. Os CDMS e CDEE e a energia metabolizável determinados com galos foram superiores aos determinados com pintos. Os maiores valores de CDMS, CDEE e de energia metabolizável foram obtidos para FSO, seguidos pelos da SIE e FSOA, e os menores, para a SID. O processo de extrusão proporcionou melhores resultados no aproveitamento da gordura do grão de soja e, conseqüentemente, da energia. Entretanto, a digestibilidade dos aminoácidos não foi influenciada pelos processamentos. Os diferentes processamentos conferiram à soja integral características nutricionais que se distinguiram, principalmente quanto ao valor de energia metabolizável, que também variou com a idade das aves.

The Amplifier - v. 6, no. 13

In this issue...Copper Lounge, Small Business Administration, Copper Guards, Marcus Daly, cheating, Student Wive's Club, Red Rooster, Frank Larvie, M- Day

The Amplifier - v. 6, no. 14

In this issue...Copper Lounge, Union Pacific Railway, Air Force, Circle K Club, Petroleum Engineers, Montana Power Company, Historical Geology Field Trip, Lime Spur Quarry

In vivo estradiol-dependent dephosphorylation of the repressor MDBP-2-H1 correlates with the loss of in vitro preferential binding to methylated DNA.

We have previously shown that estradiol treatment of roosters resulted in a rapid loss of binding activity of the repressor MDBP-2-H1 (a member of the histone H1 family) to methylated DNA that was not due to a decrease in MDBP-2-H1 concentration. Here we demonstrate that MDBP-2-H1 from rooster liver nuclear extracts is a phosphoprotein. Phosphoamino acid analysis reveals that the phosphorylation occurs exclusively on serine residues. Two-dimensional gel electrophoresis and tryptic phosphopeptide analysis show that MDBP-2-H1 is phosphorylated at several sites. Treatment of roosters with estradiol triggers a dephosphorylation of at least two sites in the protein. Phosphatase treatment of purified rooster MDBP-2-H1 combined with gel mobility shift assay indicates that phosphorylation of MDBP-2-H1 is essential for the binding to methylated DNA and that the dephosphorylation can occur on the protein bound to methylated DNA causing its release from DNA. Thus, these results suggest that in vivo modification of the phosphorylation status of MDBP-2-H1 caused by estradiol treatment may be a key step for the down regulation of its binding to methylated DNA.

Bypaths in Dixie; folk tales of the South,

First published in 1911; this edition adds an afterword.

A crime of the under-seas /

Short stories.

Comparison of apparent ileal amino acid digestibility of feed ingredients measured with broilers, layers, and roosters

The apparent ileal digestibility of amino acids in 7 feed ingredients was determined using broilers, layers, and roosters. The ingredients included 3 cereals (wheat, sorghum, and corn), 3 oilseed meals (canola, cottonseed, and soybean meals), and 1 animal protein meal (meat and bone meal). Dietary protein in the assay diets was supplied solely by the test ingredient. All diets contained 20 g/kg of acid-insoluble ash as an indigestible marker, and each diet was offered ad libitum in mash form to 5 replicate pens of broilers and layers, and 4 replicate pens of roosters. The digestibility coefficients of individual amino acids for wheat, corn, and sorghum were higher (P < 0.05) in broilers than in layers and roosters. The digestibility of most amino acids for corn and sorghum was higher (P < 0.05) in roosters compared with those in layers, whereas the digestibility for wheat in layers and roosters was similar. In general, the digestibility of amino acids in canola meal, cottonseed meal, and meat and bone meal was similar among the 3 classes of chickens. The digestibility of amino acids in soybean meal was higher (P < 0.05) for layers compared with those for broilers and roosters but similar between broilers and roosters. These results suggest that the class of chickens significantly influenced the apparent ileal digestibility of amino acids in some feed ingredients.

The Amplifier - v. 15, no. 3

In this issue...Red Rooster Supper Club, Marine Food Science, Liberal Arts, Campus Parking, The Grass Roots, Peace Corps, Europe, All Conference Football