大熊猫RPS15 cDNA的克隆及序列分析

本研究运用RT—PCR技术，首次从大熊描Ailuropoda melanoleucu的肌肉组织总RNA中成功克隆了核糖体蛋白S15（RPS15）基因的表达序列．并对其进行了初步分析。结果表明：大熊猫RPS15基因的表达序列全长为442bp，开放阅读框（ORF）为438bp，编码145个氨基酸，该蛋白的分子量为17．0401KDa，等电点为10．3，含有2个依赖于cAMP和cGMP的蛋白激酶磷酸化位点，5个蛋白激酶C磷酸化位点，4个N-酰基化位点及1个RPS19蛋白signature位点。进一步分析发现，大熊猫RPS15基因的表达序列及其编码的氨基酸序列与已报道的部分哺乳动物具有很高的相似性。

DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae).

Mealybugs (Hemiptera: Pseudococcidae) are major pests of a wide range of crops and ornamental plants worldwide. Their high degree of morphological similarity makes them difficult to identify and limits their study and management. We aimed to identify a set of markers for the genetic characterization and identification of complexes of taxa in the Pseudococcidae. We surveyed and tested the genetic markers used in previous studies and then identified new markers for particularly relevant genomic regions for which no satisfactory markers were available. We tested all markers on a subset of four taxa distributed worldwide. Five markers were retained after this first screening: two regions of the mitochondrial cytochrome oxidase I gene, 28S-D2, the entire internal transcriber space 2 locus and the rpS15-16S region of the primary mealybug endosymbiont Tremblaya princeps. We then assessed the utility of these markers for the characterization and identification of 239 samples from 43 sites in France and Brazil. The five markers studied (i) successfully distinguished all species identified by morphological examination, (ii) disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and (iii) facilitated the inference of phylogenetic relationships between the characterized taxa.