Antocianinas em extratos vegetais: aplicação em titulação ácido-base e identificação via cromatografia líquida/espectrometria de massas

Anthocyanins extracted from picao, quaresmeira, petunia, flamboyant, purple ipe, lobeira, pata de vaca, jaboticaba, purple cabbage and jambul were evaluated as natural acid-base indicators. Anthocyanins in extracts were identified using HPLC/MS. Clear, rapid change in color at final-point titration was observed for extracts but only picao showed strong change in pH. Indirect determination of carbonate in limestone using a natural indicator was performed and results compared with phenolphthalein indicator and potentiometer titration. Optimal results were obtained with picao but other extracts showed good accuracy and precision.

Substratos orgânicos para a produção de mudas de Tabebuia heptaphylla irrigadas com água potável e residuária

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Substratos orgânicos para a produção de mudas de Tabebuia heptaphylla irrigadas com água potável e residuária

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Vulnerabilidade à queda de árvores por meio de simulações microclimáticas

Muitos são os benefícios provenientes da arborização de vias públicas, como aumento da vida útil do asfalto por meio do sombreamento, filtragem de poluentes, absorção de CO2, interceptação da água da chuva e da radiação solar e mitigação de ilhas de calor; porém, por questões culturais, é considerada muitas vezes pela população como algo negativo, cujas folhas entopem calhas, raízes destroem calçadas, troncos e folhas atrapalham fiação elétrica e, principalmente, elas estão susceptíveis às quedas. A pesquisa teve como objetivo estudar o comportamento das quedas de árvores no município de Piracicaba/SP, Brasil, com atenção especial ao regime de ventos na cidade, baseado em dados fornecidos pelo Corpo de Bombeiros de Piracicaba, Secretaria de Defesa do Meio Ambiente de Piracicaba (SEDEMA) e Estação Meteorológica da Escola Superior de Agricultura \"Luiz de Queiroz\" (ESALQ). De caráter inédito, o principal resultado foi a relação direta entre quedas e zonas urbanizadas, que, em constantes alterações no uso do solo, acabam por danificar a sustentação das árvores (raízes), fato justificado pela alta concentração de quedas na parte central (36,7%), com destaque para as estações da primavera e do verão, ou seja, ventos e chuvas, na qual, juntas, somaram 78,0% do total. O período de retorno esperado para ventos superiores a 75 km h-1, classificados como temporais na Escala de Vento de Beaufort, foi de 2,8 eventos por ano. Enquanto verificou-se o predomínio de ventos alísios de sudeste, pertencentes ao quarto quadrante, a direção das rajadas de vento teve maior variação e, assim, com predomínio daqueles ventos, é recomendável instalações de parques industriais nas zonas norte, noroeste e oeste, haja vista a importância de evitar que os poluentes adentrem a cidade. As espécies de maior vulnerabilidade foram: Pachira aquatica Aubl. (monguba), Handroanthus sp. (ipê roxo) e Tipuana tipu (Benth.) Kuntze (tipuana). Por meio de simulação microclimática computacional, utilizando o programa ENVI-met versão 3.1, em dois estudos de caso, sendo um em bairro residencial e outro na Praça José Bonifácio, foi possível identificar locais de maior atenção quanto às quedas de árvores, em função da rugosidade do local, capaz de alterar a velocidade e a direção do vento.

Crystal structure of mammalian purple acid phosphatase

Background: Mammalian purple acid phosphatases are highly conserved binuclear metal-containing enzymes produced by osteoclasts, the cells that resorb bone. The enzyme is a target for drug design because there is strong evidence that it is involved in bone resorption. Results: The 1.55 Angstrom resolution structure of pig purple acid phosphatase has been solved by multiple isomorphous replacement. The enzyme comprises two sandwiched beta sheets flanked by or-helical segments. The molecule shows internal symmetry, with the metal ions bound at the interface between the two halves. Conclusions: Despite less than 15% sequence identity, the protein fold resembles that of the catalytic domain of plant purple acid phosphatase and some serine/threonine protein phosphatases. The active-site regions of the mammalian and plant purple acid phosphatases differ significantly, however. The internal symmetry suggests that the binuclear centre evolved as a result of the combination of mononuclear ancestors. The structure of the mammalian enzyme provides a basis for antiosteoporotic drug design.

Crystallization and preliminary X-ray diffraction studies of mammalian purple acid phosphatase

The oxidized form of purple acid phosphatase from pig allantoic fluid has been crystallized in the presence of phosphate using the hanging-drop technique. The crystals belong to the space group P2(1)2(1)2(1) and have unit-cell parameters a = 66.8, b = 70.3, c = 78.7 Angstrom. Diffraction data collected from a cryocooled crystal using a conventional X-ray source extend to 1.55 Angstrom resolution. A knowledge of the three-dimensional structure of mammalian purple acid phosphatase will aid in understanding the substrate specificity of the enzyme and will be important in the rational design of inhibitors, with potential in the treatment of bone diseases.

A purple acid phosphatase from sweet potato contains an antiferromagnetically coupled binuclear Fe-Mn center

A purple acid phosphatase from sweet potato is the first reported example of a protein containing an enzymatically active binuclear Fe-Mn center. Multifield saturation magnetization data over a temperature range of 2 to 200 K indicates that this center is strongly antiferromagnetically coupled. Metal ion analysis shows an excess of iron over manganese. Low temperature EPR spectra reveal only resonances characteristic of high spin Fe(III) centers (Fe(III)-apo and Fe(III)-Zn(II)) and adventitious Cu(II) centers. There were no resonances from either Mn(II) or binuclear Fe-Mn centers. Together with a comparison of spectral properties and sequence homologies between known purple acid phosphatases, the enzymatic and spectroscopic data strongly indicate the presence of catalytic Fe(III)-Mn(II) centers in the active site of the sweet potato enzyme. Because of the strong antiferromagnetism it is likely that the metal ions in the sweet potato enzyme are linked via a mu -oxo bridge, in contrast to other known purple acid phosphatases in which a mu -hydroxo bridge is present. Differences in metal ion composition and bridging may affect substrate specificities leading to the biological function of different purple acid phosphatases.

Case Report: Purple Urine Bag Syndrome

Purple urine bag syndrome (PUBS) was first reported in 1978. PUBS is rare, occurs predominantly in constipated women, chronically catheterized and associated with some bacterial urinary infections that produce sulphatase/phosphatase. The etiology is due to indigo (blue) and indirubin (red) or to their mixture that becomes purple. A chain reaction begins in the gastrointestinal tract with tryptophan as described in the article.

Structural and functional characterization of the gene products responsible for phototrophic iron oxidation by purple bacteria

Dissertação para a obtenção de grau de doutor em Bioquímica pelo Instituto de Tecnologia Química e Biológica. Universidade Nova de Lisboa

The north Pacific allies of the purple sandpiper, by Boardman Conover.

v.29:no.11(1944)

Nanoscale electrical conductivity of the purple membrane monolayer

Nanoscale electron transport through the purple membrane monolayer, a two-dimensional crystal lattice of the transmembrane protein bacteriorhodopsin, is studied by conductive atomic force microscopy. We demonstrate that the purple membrane exhibits nonresonant tunneling transport, with two characteristic tunneling regimes depending on the applied voltage (direct and Fowler-Nordheim). Our results show that the purple membrane can carry significant current density at the nanometer scale, several orders of magnitude larger than previously estimated by macroscale measurements.

Purple Wartyback Cyclonaias tuberculata, 2011

Data sheet produced by the Iowa Department of Natural Resources is about different times of animals, insects, snakes, birds, fish, butterflies, etc. that can be found in Iowa.

Yellow and purple nutsedges survey in the southeastern Buenos Aires Province, Argentina

A survey of 79 fields was conducted between December 1993 and January 1994, to determine the distribution and relative importance of species of the genus Cyperus, to justify developing management strategies in the southeastern of Buenos Aires Province, Argentina. Yellow and purple nutsedge were found in 43% and 9% respectively of the surveyed fields. Thirty eight per cent of the surveyed area showed a heavy infestation of yellow nutsedge, and in 90% of cases yellow nutsedge was invading fields cultivated with summer crops and associated with one or more of other seven perennial weeds, mainly bermudagrass.

Analyysi John "Mitch" Mitchellin rumpukomppaamisesta "Purple Haze"-sävelmän studio- ja Woodstock-versioissa.

Tässä työssä tutkittiin ja analysoitiin englantilaisen rock-rumpali John ”Mitch” Mitchellin musiikillista sanavarastoa ja rytmiikan käsittelyä Jimi Hendrixin ”Purple Haze” -sävelmän kahdessa eri versiossa.Tavoitteena oli selvittää, minkälaisista elementeistä Mitchellin komppaus muodostuu, ja mikä on hänelle tyypillinen fillaustyyli. Työmenetelminä käytettiin analyysia transkriptioaineistosta, observointia, eli tarkastelua DVD-aineistosta ja emulointia, eli soittamista tehdyistä transkriptioista. Tutkimuksen alkuun liitettiin selitykset tutkimuksessa esiintyvistä musiikillisista termeistä sekä Mitchellin biografia. Seuraavaksi analysoitiin ”Purple Haze” -sävelmän studioversio. Analyysissa sävelmä pilkottiin useampiin muutaman tahdin mittaisiin osioihin, joita kaikkia tarkasteltiin erikseen edeten järjestelmällisesti alusta loppuun. Tämän jälkeen sävelmän live-versio analysoitiin edellä mainittua menetelmää käyttäen. Lopuksi pohdittiin tiivistetysti tutkimuksen eri vaiheita ja Mitch Mitchellin soittotyyliä ”Purple Haze” -sävelmän versioissa. Tutkimuksessa todettiin Mitchellin soittotyylin olevan runsasta ja sisältävän paljon fillejä. Fillit koostuivat kuudestoistaosista, sekä kuudestoistaosatrioleista ja -sekstoleista. Woodstock -versiossa fillit koostuivat samoista aineksista ja sisälsivät kolmen kahdeksasosan tai kolmen kuudestoistaosan mittaisia sekvenssejä. Todettiin myös, että Mitchell kuunteli ja seurasi erinomaisesti Hendrixin soittoa ja reagoi hänen rytmiikan käsittelyynsä. Mitchell ja Hendrix toimivat molemmat rytmisten ärsykkeiden antajina soittaessaan.